Center for NMR spectroscopy at Rudjer Boskovic Institute (RBI) is the only academic NMR facility in Croatia. Therefore, even few hundreds of Croatian scientists and researchers from RBI and Universities of Zagreb, Rijeka, Split and Osijek, as well as other governmental institutions use permanently or temporarily the equipment of NMR center. Three NMR spectrometers are in use:

NMR center also posesses SUN Sparc station 4 and different devices for plotting, printing and retrieving of NMR data. Different kinds of measurements of ¹H and ¹³C one- and two-dimensional NMR (COSY, NOESY, ROESY, HMBC, HSQC) are performing as well as measurements of other nuclei such as: ²H, ¹⁵N, ¹⁹F, ³²P, ¹¹³Cd, ¹⁹⁹Hg. From time to time the quantitative NOE measurements and differential NOE measurements are also performing.

NMR investigations performed in Center cover organic, bioorganic and pharmaceutical chemistry. In addition inorganic, physical and analytical chemistry as well as biochemistry researches are carried out. Basic investigations are conducted on natural compounds, sugars, nucleosides and small peptides. Researches in supramolecular chemistry, metal complexation and isotope effects by NMR spectroscopy are also going on. The members of NMR center participate in Ministry of Science and Technology project No. 0098059 entitled "Nuclear Magnetic Resonance and Calculations of Bioorganic Molecules" under the conduct of D. Vikic-Topic. Within this project the NMR investigation of the structure, dynamics and interactions of small biomolecules (peptides, quinolones, etc.) and quantum chemical calculations are performed. The conformations important for bioactivity are analyzed by NOE measurements, isotope labeling and complexation. Relatively small number of atoms in these molecules enables the precise quantum chemical calculations of their structure, conformation as well as their properties. Calculated NMR parameters (chemical shifts, spin-spin couplings) are correlated with the experimental NMR data for obtaining deeper insight into the structure and nature of interactions in solution. The investigations are supported by other spectroscopy measurements (IR, UV/VIS, Mass spectrometry and X-ray analysis). The knowledge of the structure, conformation and properties of bioorganic molecules is helpful in studying bioprocesses, such as protein folding, isomerization, ligand-receptor interactions (intrinsic, extrinsic), hydrogen bonding as well as in pharmacokinetics and drug design. Short peptide fragments, 5 to 13 amino acids, can mimic the activity of the whole peptide. Such peptide fragments, which repeat many times in molecule, can be used themselves as a drug or can be the lead compounds for the development of classical drugs, since they sometimes can be even more bioactive than the whole molecule. Theoretical calculations of nuclear shielding are also performing.

In addition applicative investigations with NMR spectroscopy for pharmaceutical and petrochemical industry are carried out as well.

The staff of NMR center participates in undergraduate and graduate studies at Faculty of Science, Faculty of Pharmacy and Biochemistry, School of Health Studies, Faculty of Chemical Engineering and Technology and Faculty of Food Technology and Biochemistry of the University of Zagreb, Split and Osijek. NMR center has published few scriptae on NMR spectroscopy for undergraduate and graduate studies.

Diploma work, Master of Science and Ph. D. thesis are completed in NMR center in addition to lectures and practical demonstrations in NMR spectroscopy.