Laboratory for ichtyopatology - biological materials

Laboratory for ichtyopatology - biological materials

LIBM is conducting fundamental research (measurable through projects and publications) as well as applied research (monitoring of environmental processes, physiological disturbances, and expertise for technology transfer and know-how).  LIBM is a co-founder of the Croatian Center for Indigenous Species of Fish and Crustaceans in Karstic Waters – Otočac. LIBM members act as lecturers in graduate courses (Fisheries - Ichthyohaemathology Agriculture Faculty, University of Zagreb; and Molecular Biosciences - Fish in Biomedical Research, Rudjer Bošković Institute, University of Zagreb and J.J. Strosssmayer University of Osijek.

LIBM mission is to become regionally recognizable through multidisciplinary scientific investigations of fish, shellfish, crustaceans and reptiles within their respective environment. LIBM strength is in the long-standing scientific and professional experience, cooperation with experts within and out of the RBI, knowledge application and directing the research towards the demands of industry (pharmaceutical, food industry, aquaculture) as well as public health and environmental protection. Our overall goal is to implement the One World-One Health strategy.

LIBM is offering services of pathological/physiological status assessment of farmed and free-living fish, shellfish and crustaceans; assessment of their respective environmental quality; and conducts monitoring of populations within national parks, parks of nature and other water bodies. LIBM offers expertise for technology transfer and know-how.

On June 29 2006 founding assembly (LIBM as a member and co-founder) established Croatian Center for Indigenous Species of Fish and Crustaceans in Karstic Waters – Otočac (

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Laboratory for Ichthyopathology-Biological Materials (LIBM) was established in 2002 within the Division of Materials Chemistry, RBI as a Group for Ichthyopathology-Biological Materials. LIBM is comprised of four veterinarians-ichthyopathologists and two molecular biologists with overlapping expertise and research interests. Research interest of LIBM members are fish, crustaceans, bivalves and reptiles.

A multidisciplinary approach to the field of ichthyopathology is manifested through collaboration of experts in different disciplines to explore different areas of research using the novel tools of biochemistry, genetics, enzimology:

  • Investigation of biochemistry tissue profile as markers of physiological status and optimal living conditions to identify pathological changes and imbalanced aquatic ecosystem. This knowledge is applied to the development of diagnostic methods, treatments, and prevention tools.
  • Investigation of genotoxic effects (mycronucleus, erythrocitic nuclear abnormalities) in aquatic organisms derived from changing environmental conditions (hypoxia, chemical pollution etc.) Assessment of environmental samples (water and sediment) for genotoxicity (Ames and Ames fluctuation test).
  • Assessing impact of antimicrobials on expression and activity of fish microsomal enzymes to gained novel knowledge on pharmacokinetics.
  • Research in the field of clinical pathology (application of various methods and techniques in order to establish histological and histochemical tissue alterations; bacteriological methods in diagnostics; assessment and creation of rapid tests for differentiation of bacterial pathogens; estimations of antibiotics effects and applications; biochemical techniques; cytotoxicity assessments (algae and fish cell cultures); investigations of conditions for parasitic prevalence and incidence).
  • Investigation of the population genetics and phylogenetics and consequently preservation and revitalization of genetic diversity of aquatic organisms in open water systems. This information can be used in development of management and conservation strategies.

Rozelindra Čož-Rakovac

Senior scientist
+385 1 457 1232

Margita Jadan

+385 1 456 1097

Sandi Orlić

+385 1 457 1282

Ivančica Strunjak-Perović

senior research associate
+385 1 457 1232

Natalija Topić-Popović

senior research associate
+385 1 457 1232