Laboratory for Genotoxic Agents

Laboratory for Genotoxic Agents

 We are a young and dynamic laboratory, one of the biggest laboratories in the Division of Molecular Biology, consisting of two research groups working on different subjects with some degree of overlap. We collaborate with several researchers groups within the Institute and even more research groups abroad (Germany, France, USA, Slovenia). We use state-of-the-art cell biology, molecular biology and biochemistry techniques.

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Featured publications


  • Brozovic A., Duran G.E., Wang Y.C., Francisco B.E., Sikic B.I. The miR-200 family differentially regulates sensitivity to paclitaxel and carboplatin in human ovarian carcinoma OVCAR-3 and MES-OV cells. Molecular Oncology. in press, 2015.
  • Krüger K., Thomale J., Stojanovic N., Osmak M., Henninger C., Bormann S., Fritz G. Platinum-induced kidney damage: Unraveling the DNA damage response (DDR) of renal tubular epithelial and glomerular endothelial cells following platinum injury. Biochimica et Biophysica Acta. 1853: 685-698, 2015.
  • Majhen D., Calderon H., Chandra N., Fajardo C.A., Rajan A., Alemany R., Custers J. Adenovirus-based vaccines for fighting infectious diseases and cancer: progress in the field.Human Gene Therapy. 25, 301-317, 2014.
  • Majhen D., Stojanović N., Vukić D., Pichon C., Leduc C., Osmak M., Ambriović-Ristov A. Increased Adenovirus Type 5 Mediated Transgene Expression Due to RhoB Down-regulation, PLoS One. 9(1): e86698, 2014.
  • Moisan F., Francisco E.B., Brozovic A., Duran G.E., Wang Y.C., Chaturvedi S., Seetharamb S., Snyder L.A., Doshi P., Sikic B.I. Enhancement of Paclitaxel and Carboplatin Therapies by CCL2 Blockade in Ovarian Cancers. Molecular Oncology. 8: 1231-1239, 2014.
  • Rak S., De Zan T., Štefulj J., Kosović M., Gamulin O., Osmak M. FTIR spectroscopy reveals lipid droplets in drug resistant laryngeal carcinoma cells through detection of increased ester vibrational bands intensity. Analyst. 139, 13: 3407-3415, 2014.
  • Sommer M., Kureljak P., Urankar D., Schweinfurth D., Stojanović N., Bubrin M., Gazvoda M., Osmak M., Sarkar B., Košmrlj J. Combining [Arene–Ru] with Azocarboxamide to Generate a Complex with Cytotoxic Properties.Chemistry : A European Journal, Communication. 20: 17296-17299, 2014.
  • Vellinga J., Smith J.P., Lipiec A., Majhen D., Lemckert A., van Ooij M., Ives P., Yallop C., Custers J., Havenga M. Challenges in manufacturing adenoviral vectors for global vaccine product deployment. Human Gene Therapy. 25: 318-327, 2014.



  • Brozovic A., Vuković L., Polančac D.S., Arany I., Köberle B., Fritz G., Fiket Z., Majhen D., Ambriović-Ristov A., Osmak M. Endoplasmic reticulum stress is involved in the response of human laryngeal carcinoma cells to carboplatin but is absent in carboplatin-resistant cells. PLoS One. 8(9): e76397, 2013.
  • Rak S., Čimbora-Zovko T., Gajski G., Dubravčić K., Domijan A., Delaš I., Garaj-Vrhovac V., Batinić D.,  Sorić J., Osmak M. Carboplatin resistant human laryngeal carcinoma cells are cross resistant to curcumin due to reduced curcumin accumulation. Toxicologyin vitro. 27, 2; 523-532, 2013.
  • Watanabe S., De Zan T., Ishizaki T., Yasuda S., Kamijo H., Yamada D., Aoki T., Kiyonari H., Kaneko H., Shimizu R., Yamamoto M., Goshima G., Narumiya S. Loss of a Rho-regulated actin nucleator, mDia2, impairs cytokinesis during mouse fetal erythropoiesis. Cell Reports. 20135:926-932, 2013. 
  • Watanabe S., De Zan T., Ishizaki T., Narumiya S. Citron kinase mediates transition from constriction to abscission through its coiled-coil domain. Journal of Cell Science. 126:1773-84, 2013.
  • Gabrilovac J., Čupić B., Zapletal E., Brozović A. IFN-γ up-regulates kappa opioid receptors (KOR) on murine macrophage cell line J774. Journal of Neuroimmunology. 245, 1/2; 56-65, 2012.
  • Majhen D., Richardson J., Vukelić B., Dodig I., Cindrić M., Benihoud K., Ambriović-Ristov A. The disulfide bond of an RGD4C motif inserted within the HI loop of the adenovirus type 5 fiber protein is critical for retargeting to αv-integrins. Journal of Gene Medicine. 14(12): 788-797, 2012.
  • Osmak M. Statins and cancer : Current and future prospects. Cancer Letters. 324, 1; 1-12, 2012.



  • Čimbora-Zovko T., Brozović A., Piantanida I., Fritz G., Virag A.,  Alič B., Majce V., Kočevar M., Polanc S., Osmak M. Synthesis and biological evaluation of 4-nitro-substituted 1, 3-diaryltriazenes as a novel class of potent antitumor agents. European Journal of Medicinal Chemistry. 46: 2971-2983, 2011.
  • Gabrilovac J., Čupić B.,Živković E., Horvat L., Majhen D. Expression, regulation and functional activities of aminopeptidase N (EC ; APN ; CD13) on murine macrophage J774 cell line. Immunobiology. 216, 1/2; 132-144, 2011.
  • Helbig L., Damrot J., Hülsenbeck J., Köberle B., Brozović A., Osmak M., Fiket Ž., Kaina B., Fritz G. Late activation of stress-activated protein kinases/C-JUN-N- terminal kinases triggered by cisplatin-induced DNA damage repair defective cells. The Journal of Biological Chemistry. 286, 15; 12991-13001, 2011.
  • Majhen D., Stojanović N., Špeljko T., Brozović A., De Zan T., Osmak M., Ambriović-Ristov A. Increased expression of the coxsackie and adenovirus receptor downregulates alpha v beta 3 and alpha v beta 5 integrin expression, reduces cell adehesion and migration. Life Sciences. 89: 241-249, 2011.
  • Brozovic A., Ambriović-Ristov A., Osmak M. The relationship between cisplatin-induced reactive oxygen species, glutathione and BCL-2, and resistance to cisplatin. Critical Reviews in Toxicology. 40(4): 347-359, 2010.
  • Burja B., Čimbora-Zovko T., Tomić S., Jelušić T., Kočevar M., Polanc S., Osmak M. Pyrazolone-fused combretastatins and their precursors : synthesis, cytotoxicity, antitubulin activity and molecular modeling studies. Bioorganic & medicinal chemistry. 18: 2375-2387, 2010.
  • Cimbora-Zovko T, Fritz G, Mikac N, Osmak M. Downregulation of RhoB GTPase confers resistance to cisplatin in human laryngeal carcinoma cells. Cancer Letters.  295:182-190, 2010.



  • Brozović A., Damrot J., Tsaryk R., Helbig L., Nikolova T., Hartig C., Osmak M., Roos W., Kaina B., Fritz G. Cisplatin sensitivity is related to late DNA damage processing and checkpoint control rather than to the early DNA damage response. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 670, 1-2; 32-41, 2009.
  • Majhen D., Nemet J., Richardson J., Gabrilovac J., Hajsig M., Osmak M., Eloit M., Ambriović-Ristov A. Differential role of αvβ3 and αvβ5 integrins in internalization and transduction efficacies of wild type and RGD4C fiber-modified adenoviruses. Virus Research. 139(1): 64-73, 2009.
  • Brozović A., Majhen D, Roje V., Mikac N., Jakopec S., Fritz G., Osmak M., Ambriović-Ristov A. αvβ3 integrin mediated drug resistance in human laryngeal carcinoma cells is caused by glutathione dependent elimination of drug induced reactive oxidative species. Molecular Pharmacology. 74: 298-306, 2008.



  • Brozović A., Osmak M. Activation of mitogen-activated protein kinases by cisplatin and their role in cisplatin-resistance. Cancer Letters. 251 (2007) , 1; 1-16, 2007.
  • Čimbora-Zovko T.,  Ambriović-Ristov A.; Lončarek  J., Osmak M.: Altered cell-cell adhesion in cisplatin-resistant human carcinoma cells: A link between b-catenin/plakoglobin ratio and cisplatin resistance. European Journal of Pharmacology. 558: 27-36, 2007.
  • Zisowsky J., Koegel S., Leyers S., Devarakonda K., Kassack M., Osmak M., Jaehde U. Relevance of drug uptake and efflux for platinum sensitivity of tumour cells. Biochemical Pharmacology. 73 , 2; 298-307, 2007.
  • Jakopec S., Dubravčić K.,  Polanc S.,  Košmrlj J., Osmak M.: Diazene JK-274 induces apoptosis-like cell death in human cervical carcinoma cells. Toxicology In Vitro. 20:217-226, 2006.
  • Majhen D., Ambriović-Ristov, A. Adenoviral vectors-how to use them in cancer gene therapy, Virus Research. 119: 121-133, 2006.
  • Majhen D., Gabrilovac J., Eloit M., Richardson J., Ambriović-Ristov A. Disulfide bond formation in NGR fiber-modified adenovirus is essential for retargeting to aminopeptidase N. Biochemical and Biophysical Research Communications. 348: 278-287, 2006.

Andreja Ambriović Ristov

Senior Scientist
+385 1 457 1240

Anamaria Brozović

scientific associate
+385 1 456 1145

Tihana De Zan
B. Sc.

Research Fellow, Assistant
+385 1 456 1145

Dragomira Majhen

research associate
+385 1 456 1145

Maja Osmak
Ph. D.

Senior Scientist
+385 1 456 0939

Sanjica Rak

+385 1 457 1293

Nikolina Stojanović

senior research assistant
+385 1 456 1145