Andreja Ambriović Ristov

Andreja Ambriović Ristov

Ph.D.

+385 1 457 1240

1543
1545
Senior Scientist

Krilo 5/9

Laboratory for Genotoxic Agents
Division of Molecular Biology
Ruđer Bošković Institute
Bijenička 54
10000 Zagreb
Croatia

Education

1989, Zagreb, Croatia, Faculty of Pharmacy and Biochemistry, University of  Zagreb, B. Sc.(clinical chemistry)
1993, Zagreb, Croatia, Faculty of Science, University of  Zagreb, Mr.Sc. (biology)
1997, Zagreb, Croatia, Faculty of Science, University of  Zagreb, Ph. D. (biology)

Projects

(2014-2018) HRZZ Research Project
„Molecular mechanisms of chemosensitisation of human breast carcinoma and melanoma cell lines by silencing of integrins“
Role on the project: Leader

(2014-2015): bilateral project with Germany
”Sensitization of melanoma and glioma cells to alkylating drugs by integrin αvβ3, αvβ5, α3β1 and α4β1 silencing”
Role on the project:  Leader

(2013-2016) FP7-REGPOT project
”Enhancement of the Innovation Potential in SEE through New Molecular Solutions in Research and Development” leader dr. sc. Oliver Vugrek
Role on the project:  Co-worker
 
(2012-2013) bilateral project with Germany
„Platinum compounds: Drug-induced stress responses and mechanisms of drug- resistance“ Institute for toxicology, Heinrich Heine University Düsseldorf, Düsseldorf,  Germany
Role on the project:  Co-worker

(2008-2009) bilateral project with Germany
„Molecular mechanisms that deterimines cell sensitivity to platinum compounds” Institut for toxicology, Johannes Gutenberg University, Mainz, Germany
Role on the project:  Co-worker

(2007-2013) Ministry of Science, Education and Sports, “Increase of adenovirus transduction efficacy and resistance to cytostatics”
Role on the project:  Leader

(2007-2013) Ministry of Science, Education and Sports, “Cell respones to cytotoxic agents and resistance development “
Role on the project:  Co-worker

(2007-2010) Technological project awarded by Ministry of Science, Education and Sports, “Regeneration of knee articular cartilage”
Role on the project: Leader

(2004-2006) Integrated action: Ministry of Science, Education and Sports and French Government: COGITO, “Adenovirus retargeting to aminopeptidase N and potential use in gene therapy of cystic fibrosis”
Role on the project: Leader

(2002-2004) NATO Science Program, Collaborative linkage grant, “Human adenovirus type 5 retargeted on aminopeptidase N”
Role on the project: Leader

(1990-1995) Ministry of Science, Education and Sports Science and Technology  
“The investigation of cell resistance to genotoxic agents”
Role on the project: Co-worker

(1998-2000) Ministry of Science, Education and Sports,  Young researcher grant
“Vector vaccines against pseudorabies virus”
Role on the project:  Leader

(1996-2002) Ministry of Science, Education and Sports, Cell response to physical, chemical and biological noxa
Role on the project: Co-worker

Awards and Achievements

2008. Award J. J. Strossmayer (Croatian Academy of Sciences and Arts/ Zagreb fair), for the best publishing venture in science, for the book «Methods in molecular biology» by Andreja Ambriović Ristov (Editor-in-chief) and Anamaria Brozović, Branka Bruvo Mađarić, Helena Ćetković, Maja Herak Bosnar, Dubravka Hranilović, Silva Katušić, Hećimović, Nevenka Meštrović Radan, Snježana Mihaljević, Neda Slade, Dušica Vujaklija (Eds.).

2004. The award of director of Ruđer Bošković Institute for contribution in Institute development and common prosperity.

Classes

2008-, Course leader «Gene therapy», graduate Studies programme in Molecular Biotechnology, Faculty of Food Technology and Biotechnology, University of Zagreb.

2008-, Course leader «Methods in molecular biology and medicine», The Ph.D. study in biophysics, Faculty of Science, University of Split.

2007-, Course co-leader «Methods in molecular biology and medicine», PhD Studies in Molecular Biosciences, University of Osijek, University of Dubrovnik and Ruđer Bošković Institute,

2007-, Course co-leader «Methods in molecular biology and medicine-practicum», PhD Studies in Molecular Biosciences, University of Osijek, University of Dubrovnik and Ruđer Bošković Institute.

2002-, Course Leader «Molecular basis of Gene Therapy» PhD Studies in biology, Faculty of Science, University of Zagreb.

Featured Publications

Majhen D., Stojanović N., Vukić D., Pichon C., Leduc C., Osmak M., Ambriović-Ristov A. Increased Adenovirus Type 5 Mediated Transgene Expression Due to RhoB Down-regulation, PLoS One, 9(1): e86698, 2014.

Brozovic A., Vuković L., Polančac D.S., Arany I., Köberle B., Fritz G., Fiket Z., Majhen D., Ambriović-Ristov A., Osmak M. Endoplasmic reticulum stress is involved in the response of human laryngeal carcinoma cells to carboplatin but is absent in carboplatin-resistant cells. PLoS One, 8(9): e76397, 2013.

Majhen D., Richardson J., Vukelić B., Dodig I., Cindrić M., Benihoud K., Ambriović-Ristov A. The disulfide bond of an RGD4C motif inserted within the HI loop of the adenovirus type 5 fiber protein is critical for retargeting to αv-integrins. Journal of Gene Medicine, 14(12): 788-797, 2012.

Majhen D., Stojanović N., Špeljko T., Brozović A., De Zan T., Osmak M., Ambriović-Ristov A. Increased expression of the coxsackie and adenovirus receptor downregulates alpha v beta 3 and alpha v beta 5 integrin expression, reduces cell adehesion and migration. Life Sciences, 89(7-8): 241-249, 2011.

Brozovic A., Ambriović-Ristov A., Osmak M. The relationship between cisplatin-induced reactive oxygen species, glutathione and BCL-2, and resistance to cisplatin. Critical reviews in toxicology, 40(4): 347-359, 2010.
Majhen D., Nemet J., Richardson J., Gabrilovac J., Hajsig M., Osmak M., Eloit M., Ambriović-Ristov A. Differential role of αvβ3 and αvβ5 integrins in internalization and transduction efficacies of wild type and RGD4C fiber-modified adenoviruses. Virus Research. 139(1): 64-73, 2009.

Brozović A., Majhen D, Roje V., Mikac N., Jakopec S., Fritz G., Osmak M., Ambriović-Ristov A. αvβ3 integrin mediated drug resistance in human laryngeal carcinoma cells is caused by glutathione dependent elimination of drug induced reactive oxidative species. Molecular Pharmacology. 74: 298-306, 2008.

Žaja R., Munić V., Sauerborn Klobučar R., Ambriović-Ristov A., Smital T. Cloning and molecular characterization of apical efflux transporters (ABCB1, ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicology, 90(4): 322-332, 2008.

Majhen D., Ambriović-Ristov, A. Adenoviral vectors-how to use them in cancer gene therapy, Virus Research, 119: 121-133, 2006.

Majhen D., Gabrilovac J., Eloit M., Richardson J., Ambriović-Ristov A. Disulfide bond formation in NGR fiber-modified adenovirus is essential for retargeting to aminopeptidase N. Biochemical and Biophysical Research Communications. 348: 278-287, 2006.

Ambriović-Ristov A., Gabrilovac J., Čimbora-Zovko T., Osmak M. Increased adenoviral transduction efficacy in human laryngeal carcinoam cells resistant to cisplatin isassociated with increased expression of integrin αvβ3 and coxsackie adenovirus receptor, International Journal of Cancer, 110: 660-667, 2004.

Ambriović-Ristov A., Mercier S., Eloit M. Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells, Virology, 312: 425-433, 2003.

Monteil M., Le Pottier M. F., Ambriović Ristov A., Cariolet R., L'Hospitalier R., Klonjkowski B., Eloit M. Single inoculation of replication-defective adenovirus-vectored vaccines at birth in piglets with maternal antibodies induces high level of antibodies and protection against pseudorabies, Vaccine, 18: 1738-1742, 2000.

Chetboul V., Adam M., Deprez I., Ambriović A., Rosenberg D., Crespeau F., Saana M., Pham I., Eloit M., Adnot S., Pouchelon JL. Expression of biologically active atrial natriuretic factor following intrahepatic injection of a replication-defective adenoviral vector in dogs, Human Gene Therapy, 10(2):281-290, 1999.

Ambriović A., Adam M., Monteil M., Paulin D., Eloit M. Efficacy of replication-defective adenovirus vectored vaccines: Protection following intramuscular injection is linked to promoter efficiency in muscle representative cells, Virology 238: 327-335, 1997.

Membership in professional associations / societies

Croatian Society for Biochemistry and Molecular Biology (thus member of FEBS)
Croatian Microbiological Society (thus member of FEMS)
Croatian society of genetic engineers
Croatian Association for Cancer Research
Croatian Genetic Society

Miscellaneous

2003-2013 The founder and director of educational project “Methodological Courses in Biology and Medicine”

Editor in chief of a book “Methods in molecular biology” with contributions from 119 authors from Ruđer Bošković Institute and University which has been accepted as a regular textbook by the University in Zagreb. Institut Ruđer Bošković (Editor), 2007, Zagreb. http://www.mmb.irb.hr/

MENTORSHIP

Doctoral Thesis

1) Nikolina Stojanović, „Integrin αvβ3 mediated drug resistance in human tongue squamous carcinoma cells“, Josip Juraj Strossmayer University of Osijek, University of Dubrovnik, Ruđer Bošković Institute, Universitypostgraduate interdisciplinary Doctoral Study of Molecular biosciences, 2014.

2) Dragomira Majhen, „Augmentation of adenovirus transduction: Changing expression of natural receptors and retargeting to new ones“, Faculty of Science University of Zagreb, 2008.

3) Ivana Steiner, in progress

Master of Science Thesis:

1) Dragomira Majhen: «Adenovirus type 5 retargeting on amniopeptidase N», Faculty of Science University of Zagreb, 2005.

Diploma Thesis

1) Ivan Faraho, „Differential proteome analysis of Cal27 and Cal27-2B1 human tongue squamous cell carcinoma cell lines“, Faculty of Science, University of Zagreb, 2015. comenthors Andreja Ambriović-Ristov and Mario Cindrić
2) Alen Kovačević, „Proteomic analysis of human breast cancer cells MDA-MB-435S after silencing integrin alpha v“, Faculty of Science, University of Zagreb, 2015. comenthors Andreja Ambriović-Ristov and Mario Cindrić
3) Kristijan Pažur, „Disulfide bonds in retargeting motifs RGD or NGR inserted into the adenovirus type 5“, Faculty of Science, University of Zagreb, 2013
4) Krešimir Ferenčak, „Effect of integrin αv or α4 silencing on sensitivity of human breast cancer cells to vincristine and paclitaxel“, Faculty of Science, University of Zagreb, 2013
5) Buger Tvrtko, „Vincristine cell resistance is accompanied by changes in integrin expression and adenovirus transduction efficiency“, Faculty of Science, University of Zagreb, 2012
6) Dekanić Ana, „Influence of integrin αvβ3 and αvβ5 silencing on sensitivity of tumor cells to cisplatin“, Faculty of Science, University of Zagreb, 2012
7) Irena Bardak, „Influence of αvβ3 and αvβ5 integrin silencing on attachment, internalization and transduction with adenoviral vectors“, Faculty of Science, University of Zagreb, 2011
8) Dunja Vukić, „Increased Ad5-mediated transgene expression in cisplatin-resistant human laryngeal carcinoma cells“, Faculty of Pharmacy and Biochemistry, University of Zagreb, 2010
9) Tea Špeljko, „The influence of the coxsackie adenovirus receptor expression on integrin expression in human rhabdomyosarcoma cell line“, Faculty of Science, University of Zagreb, 2010
10) Tihana Jelušić, „Retargeting adenovirus type 5 to integrin α4β1“, Faculty of Science, University of Zagreb, 2009
11) Nikolina Stojanović, „αvβ3 integrin mediated drug resistance in human tongue squamous cell carcinoma“, Faculty of Science, University of Zagreb, 2009

VISITS TO FOREIGN RESEARCH INSTITUTIONS

Visiting professor (duration 1 month) year 2013/2014: University of Orleans, France.
Visiting professor (duration 1 month) year 2010/2011: University of Orleans, France.

INRA postdoc fellowship; March-August, 2001; Ecole Nationale Veterinaire D’Alfort, 7 avenue du General de Gaulle, 94704 Maisons Alfort, France.

French Government Fellowship: November, 1994 - August, 1995, Ecole Nationale Veterinaire D’Alfort, 7 avenue du General de Gaulle, 94704 Maisons Alfort, France.

Laba