Andreja Ambriović Ristov
Laboratory for Genotoxic Agents
Division of Molecular Biology
Ruđer Bošković Institute
1989, Zagreb, Croatia, Faculty of Pharmacy and Biochemistry, University of Zagreb, B. Sc.(clinical chemistry)
1993, Zagreb, Croatia, Faculty of Science, University of Zagreb, Mr.Sc. (biology)
1997, Zagreb, Croatia, Faculty of Science, University of Zagreb, Ph. D. (biology)
(2007-) Ministry of Science, Education and Sports, “Increase of adenovirus transduction efficacy and resistance to cytostatics”, Leader
(2007-2009) Technological project awarded by Ministry of Science, Education and Sports, “Regeneration of knee articular cartilage”, Leader
(2006-2008) Integrated action: Ministry of Science, Education and Sports and French Government: COGITO, “Adenovirus retargeting to aminopeptidase N and potential use in gene therapy of cystic fibrosis”, Co-leader
(2002-2004) NATO Science Program, Collaborative linkage grant, “Human adenovirus type 5 retargeted on aminopeptidase N”, Co-leader
(1998-2000) Ministry of Science, Education and Sports, Young researcher grant
“Vector vaccines against pseudorabies virus”, Leader
Awards and Achievements
2008. Award J. J. Strossmayer (Croatian Academy of Sciences and Arts/ Zagreb fair), for the best publishing venture in science, for the book «Methods in molecular biology» by Andreja Ambriović Ristov (Editor-in-chief) and Anamaria Brozović, Branka Bruvo Mađarić, Helena Ćetković, Maja Herak Bosnar, Dubravka Hranilović, Silva Katušić, Hećimović, Nevenka Meštrović Radan, Snježana Mihaljević, Neda Slade, Dušica Vujaklija (Eds.).
2004. The award of director of Ruđer Bošković Institute for contribution in Institute development and common prosperity.
2008-, Course leader «Gene therapy», graduate Studies programme in Molecular Biotechnology, Faculty of Food Technology and Biotechnology, University of Zagreb.
2008-, Course leader «Methods in molecular biology and medicine», The Ph.D. study in biophysics, Faculty of Science, University of Split.
2007-, Course co-leader «Methods in molecular biology and medicine», PhD Studies in Molecular Biosciences, University of Osijek, University of Dubrovnik and Ruđer Bošković Institute,
2007-, Course co-leader «Methods in molecular biology and medicine-practicum», PhD Studies in Molecular Biosciences, University of Osijek, University of Dubrovnik and Ruđer Bošković Institute.
2002-, Course Leader «Molecular basis of Gene Therapy» PhD Studies in biology, Faculty of Science, University of Zagreb.
Majhen D., Stojanović N., Špeljko T., Brozovic A., De Zan T., Osmak M., Ambriović-Ristov A. Increased expression of the coxsackie and adenovirus receptor downregulates αvβ3 and αvβ5 integrin expression, reduces cell adhesion and migration. Life sciences, 89 (2011), 7-8; 241-249.
Brozovic A., Ambriović-Ristov A., Osmak M. The relationship between cisplatin-induced reactive oxygen species, glutathione and BCL-2, and resistance to cisplatin. Critical reviews in toxicology. 40 (2010), 4; 347-359.
Majhen D., Nemet J., Richardson J., Gabrilovac J., Hajsig M., Osmak M., Eloit M., Ambriović-Ristov, A. Differential Role of αvβ3and αvβ5Integrins in Internalization and Transduction Efficacies of Wild Type and RGD4C Fiber-modified Adenoviruses. Virus Research. 139 (2009), 1; 64-73.
Brozovic A., Majhen D., Roje V., Mikac N., Jakopec S., Fritz G., Osmak M., Ambriović-Ristov A. αvβ3 integrin mediated drug resistance in human laryngeal carcinoma cells is caused by glutathione dependent elimination of drug induced reactive oxidative species. Molecular Pharmacology. 74 (2008), 1; 298-306.
Majhen D., Ambriović-Ristov A.Adenoviral vectors-How to use them in cancer gene therapy? Virus Research. 119 (2006); 121-133.
Majhen D., Gabrilovac J., Eloit M., Richardson J., Ambriović-Ristov A. Disulfide bond formation in NGR fiber-modified adenovirus is essential for retargeting to aminopeptidase N. Biochemical and Biophysical Research Communications. 348 (2006); 278-287.
Ambriović-Ristov A., Gabrilovac J., Čimbora Zovko T., Osmak M. Increased adenoviral transduction efficacy in human laryngeal carcinoma cells resistant to cisplatin is associated with increased expression of integrin avb3 and coxsackie adenovirus receptor. International Journal of Cancer. 110 (2004); 660-667.
Ambriović-Ristov A., Mercier S., Eloit M. Shortening adenovirus type 5 fiber shaft decreases the efficiency of post binding steps in CAR-expressing and non-expressing cells. Virology. 312 (2003); 425-433.
Chetboul V., Adam M., Deprez I., Ambriović-Ristov A., Rosenberg D., Crespeau F., Saana M., Pham I., Eloit M., Adnot S., Pouchelon J-L. Expression of biologically active atrial natriuretic factor following intrahepatic injection of a replication-defective adenoviral vector in dogs. Human gene therapy. 10 (1999), 2; 281-290.
Ambriović A., Adam M., Monteil M., Paulin D., Eloit M. Efficacy of replication-defective adenovirus-vectored vaccines: protection following intramuscular injection is linked to promoter efficiency in muscle representative cells. Virology. 238 (1997), 2; 327-335.
Membership in professional associations / societies
Croatian Society for Biochemistry and Molecular Biology (thus member of FEBS)
Croatian Microbiological Society (thus member of FEMS)
Croatian society of genetic engineers
Croatian Association for Cancer Research
Croatian Genetic Society
2003- The founder and director of educational project “Methodological Courses in Biology and Medicine” http://tecajevi-irb.com/
Editor in chief of a book “Methods in molecular biology” with contributions from 119 authors from Ruđer Bošković Institute and University which has been accepted as a regular textbook by the University in Zagreb. Institut Ruđer Bošković (Editor), 2007, Zagreb. http://www.mmb.irb.hr/
1) Dragomira Majhen: «Augmentation of adenovirus transduction: Changing expression of natural receptors and retargeting to new ones», Faculty of Science University of Zagreb, 2008.
2) Nikolina Stojanović, in progress
Master of Science Thesis:
1) Dragomira Majhen: «Adenovirus type 5 retargeting on amniopeptidase N», Faculty of Science University of Zagreb, 2005.
1) Irena Bardak: «Influence of αvβ3 and αvβ5 integrin silencing on attachment, internalization and transduction with adenoviral vectors», Faculty of Science, University of Zagreb, 2011.
2) Dunja Vukić: «Increased Ad5-mediated transgene expression in cisplatin-resistant human laryngeal carcinoma cells», Faculty of Pharmacy and Biochemistry, University of Zagreb, 2010.
3) Tea Špeljko: «The influence of the coxsackie adenovirus receptor expression on integrin expression in human rhabdomyosarcoma cell line», Faculty of Science, University of Zagreb, 2010.
4) Tihana Jelušić: «Retargeting adenovirus type 5 to integrin α4β1», Faculty of Science, University of Zagreb, 2009.
5) Nikolina Stojanović: «αvβ3 integrin mediated drug resistance in human tongue squamous cell carcinoma», Faculty of Science, University of Zagreb, 2009.
VISITS TO FOREIGN RESEARCH INSTITUTIONS
Invited researcher, University of Orleans, Orleans France, one month during 2011.
INRA postdoc fellowship; March-August, 2001; Ecole Nationale Veterinaire D’Alfort, 7 avenue du General de Gaulle, 94704 Maisons Alfort, France.
French Government Fellowship: November, 1994 - August, 1995, Ecole Nationale Veterinaire D’Alfort, 7 avenue du General de Gaulle, 94704 Maisons Alfort, France.