Acquisition and interpretation of NMR spectra

Acquisition and interpretation of NMR spectra

NMR spectrometer Bruker Avance 600 equipped with superconducting magnet (14 T) and two probes for liquid samples, each containing a broadband channel. Covered frequency range of 24 - 600 MHz enables analysis of the majority of isotopes (from Ag-107 to H-1). Measurements are possible in the temperature range of 223 - 373 K.

Spectroscopy of nuclear magnetic resonance (NMR spectroscopy) is research method employing magnetic properties of particular atomic nuclei to analyse corresponding molecular physical and chemical properties. It is based on nuclear magnetic resonance phenomenon and provides detailed information on molecular structure, dynamics and chemical environment.

NMR spectroscopy is predominantly used by chemists and biochemists to investigate properties of organic molecules. Small compounds could be analysed by simple one-dimensional techniques H-1 and C-13, while for bigger molecules like proteins or nucleic acids multidimensional techniques are necessary.