Contents

1. Structural Basis of Protein Synthesis

Christiane Schaffitzel and Nenad Ban                              

2. The Relationship Between Protein Sequence, Structure and Function: Protein Function Prediction

Anna Tramontano and Domenico Cozzetto                         

3. Differences in Binding of Stereoisomers to Protein Active Sites

Gerhard Klebe                                                          

4. Analytical Centrifugation: Looking at Aggregation in Free Solution

P. Jonathan G. Butler                                                        

5. Time Resolved Protein Fluorescence. Application to Multi-Tryptophan Proteins

Yves Engelborghs                                                              

6. Novel (Bio)chemical and (Photo)physical Probes for Imaging Living Cells

Elizabeth A. Jares-Erijman, Carla Spagnuolo, Luciana Giordano, Maria Etchehon, Jennifer Kawior, Maria V. Mañalich-Arana, Mariano Bossi, Diane S. Lidke, Janine N. Post, Rudolf J. Vermeij, Rainer Heintzmann, Keith A. Lidke, Donna J. Arndt-Jovin, Thomas M. Jovin                                                                    

7. Observing Structure and Dynamics of Membrane Proteins by High-resolution Microscopy

Andreas Engel                                                         

8. A 2D-Infrared Study of Human Lipoproteins

Xabier Cotto, Ibón Iloro, and José Luis R. Arrondo             

9. An Introduction to Biological Solid State NMR

Andrew Dodd and Frances Separovic                               

10. Multi-Frequency EPR Spectroscopy Studies of the Structure and Conformational Changes of Site-Directed Spin Labbeled Membrane Proteins

Heinz-Jürgen Steinhoff                                                        

11. Identification of Protein Structure and its Modifications by Electrospray Mass Spectrometry in Proteomics

Jasna Peter-Katalinić                                                

12. A Microscopic Study of Disorder-Order Transitions in Molecular Recognition of Unstructured Proteins: Hierarchy of Structural Loss and the Transition State Determination from Monte Carlo Simulations of P27KIP1 Protein Coupled Unfolding and Unbinding

Gennady M. Verkhivker                                                       

13. Computational Detection of the Binding Site Hot Spot and Predicting Energetics of Ligand Binding at the Remodeled Human Growth Hormone–Receptor Interface Using a Hierarchy of Molecular Docking and Binding Free Energy Approaches

Gennady M. Verkhivker                                                       

14. Molecular and Cellular Levels of Biological Evolution

Miroslav Radman