Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu (AMBIOMERES)

Utjecaj okolišnog stresa na pojavnost i međudjelovanje biološki važnih organskih molekula i mikronutrijenata u morskom ekosustavu (AMBIOMERES)
Datum početka projekta
Datum kraja projekta
Kategorija projekta
Projekti Hrvatske zaklade za znanost

Recently documented oligotrophication of the Adriatic Sea caused by global changes opened questions how this reflects on the quality and quantity of organic matter (OM) produced by the phytoplankton and on the consequent influence on the micronutrient metals availability and cycling. Within the project, a progressive (model and in-situ) approach will be accomplished to reveal crucial alterations in the marine ecosystem. Initial investigations will imply model experiments on the interaction of selected OM (polysaccharides, lipids, polymeric organic matter containing N catalytic atoms) with micronutrient metals (Fe, Co, Cu, Zn and Cd). Additionally, phytoplankton monoculture incubation under various growth conditions (nutrient concentrations and temperature variations) will be included in order to comprehend multiple impacts on the phytoplankton OM production and micronutrients metals distribution. Model experiments will be followed by in-situ investigations at the selected oligotrophic and eutrophic areas of the Adriatic Sea. This will give holistic informations on the natural marine biochemical cycles and linkage between OM and micronutrient metals. In-situ investigations will be focused on the seasonal variations of the OM and micronutrient appearance and concentrations in combination with physico-chemical parameters (temperature, salinity, pH, O2), and dissolved and particulate organic carbon content, surface-active organic substances and macronutrients (PO4, NO3, NO2, NH4). These investigations will be conducted by intercomparable and multimethodological approaches using various chromatographic to electrochemical techniques. Finally, taking into account all obtained results an important part of the natural marine ecosystem processes will be characterized in the prospective of climate changes.  


Simplified schematic of assumed interactions between micronutrient metals (M=Fe, Cu, Co, Zn, Cd) and organic molecules (lipids, acidic polysaccharides and N-POM) released from phytoplankton in conditions of (A) oligotrophy and (B) eutrophy. Biological processes drive production of OM and speciation of micronutrient metals:

(A) Micronutrient metals (hydrated ionic form, M, or inorganic complexes, MX) interact with released OM forming complexes from which cells easily take micronutrient metal by ligand exchange reactions. This is mechanism through which OM enhance bioavailability of micronutrient metals in deficiency.

(B) In eutrophy phytoplankton produce altered organics that form complexes with micronutrient metals which do not enable micronutrient metal uptake by cell. This is mechanism through which OM is reducing toxicity of micronutrient metals when they are in surplus. There are enough micronutrients in inorganic form which are preferable for the cellular uptake.



6.10.2014 - "Kickoff" sastanak 

19.09.2016 održana radionica na Institutu Ruđer Bošković. Program radionice se nalazi u priloženoj brošuri.

ambiomeres brosura 1
ambiomeres brosura 2



Mlakar, Marina; Fiket, Željka; Geček, Sunčana; Cukrov, Neven; Cuculić, Vlado.
Marine lake as in situ laboratory for studies of organic matter influence on speciation and distribution of trace metals. // Continental shelf research. 103 (2015) ; 1-11

Gašparović, Blaženka; Kazazić, Snježana P.; Cvitešić, Ana; Penezić, Abra; Frka, Sanja.
Improved separation and analysis of glycolipids by Iatroscan thin–layer chromatography–flame ionization detection. // Journal of chromatography. A. 1409 (2015) ; 259-267

Plavšić, Marta; Strmečki Kos, Sladjana.
Carbohydrate polymers as constituents of exopolymer substances in seawater, their complexing properties towards copper ions, surface and catalytic activity determined by electrochemical methods. // Carbohydrate polymers. (2015)

Mlakar, M., Pavković, S., Tumir, L-M., Vukosav, P., Gašparović, B. 2017. Electrochemical characterization of biochemically active Cu(II) mixed ligand complex with histidine and cysteine. Electroanalysis 29, 392-397.

Vukosav, P., Mlakara, M., Selmani, A., Bregović, N., Gašparović, B. 2016. Electroanalytical  characterization  of  Zn  (II)  complexes  with D-mannosamine  and  Glycine  in  aqueous  solutions. Electrochim.  Acta 188, 671–678.

Orlović-Leko, P.; Vidović, K.; Plavšić, M.; Ciglenečki, I.; Šimunić, I.; Minkina T.: Voltammetry as a tool for rough and rapid characterization of dissolved organic matter in the drainage water of hydroameliorated agricultural areas in Croatia. Journal of Solid State Electrochemistry, 2016, DOI 10.1007/s10008-016-3245-0.

Novak, T., Godrijan, G., Marić Pfannkuchen, D., Djakovac, T., Mlakar, M., Baricevic, A., Smodlaka Tanković, M., Gašparović, B. 2018. Enhanced dissolved lipid production as a response to the sea surface warming. J. Mar. Sys. 180, 289–298.

Mlakar, M., Cuculić, V., Frka, S., Gašparović, B. 2018. Copper - phospholipid interaction at cell membrane model hydrophobic surfaces. Bioelectrochem. 120, 10–17.

Strmečki, S., Paleček., E. 2018. dsorption/desorption of biomacromolecules involved in catalytic hydrogen evolution. Bioelectrochem. 120, 87-93.



Blaženka Gašparović, Abra Penezić, Richard S. Lampitt, Nilusha Sudasinghe, Tanner Schaub: Phospholipid Characterization as a Component of the Oceanic Phosphorus and Carbon Cycle. 2018 Ocean science meeting, Portland, Oregon, SAD, https://agu.confex.com/agu/os18/meetingapp.cgi/Paper/318176

Marina Mlakar, Vlado Cuculić, Sanja Frka Milosavljević, Blaženka Gašparović, Sanja Frka: Copper - Phospholipid Interaction at Cell Membrane Model Hydrophobic Surfaces. 2018 Ocean science meeting, Portland, Oregon, SAD, https://agu.confex.com/agu/os18/meetingapp.cgi/Paper/317752

Blaženka Gašparović, Tihana Novak, Jelena Godrijan, Marina Mlakar, Daniela Marić, Tamara Djakovac: Dissolved lipid production in the Northern Adriatic (Mediterranean) in response to sea surface warming. European Geosciences Union General Assembly 2017, Beč, Austrija, 23.-28. 04. 2017. http://meetingorganizer.copernicus.org/EGU2017/posters/23131

Tihana Novak, Blaženka Gašparović, Jelena Godrijan, Daniela Marić, Tamara Djakovac, Marina Mlakar, Effects of temperature on growth and lipid synthesis of diatom Chaetoceros Curvisetus and the Northern Adriatic plankton community, European Geosciences Union General Assembly 2017, Beč, Austrija. http://meetingorganizer.copernicus.org/EGU2017/posters/23131

Marina Mlakar, Biogeochemistry and speciation of trace elements in karst marine lakes. Zuerich, Švicarska, 16-20. 07.2017. http://icobte2017.ch/images/docs/EnvTE_Aquatic.pdf

Slađana Strmečki Kos, Emil Paleček, Tensammetry of proteins and polysaccharides on mercury electrode under conditions of catalytic activity, 6th RSE-SEE Conference 2017, Balatonkenese, Mađarska, 11-15.06.2017. http://rse-see2017.hu/

Daniela Marić Pfannkuchen, Ingrid Ivančić, Mirta Smodlaka Tanković, Ana Baričević, Tihana Sesar, Blaženka Gašparović, Martin Pfannkuchen, Life strategies of harmful planktonic algae (with respect to phosphate limitation) in marine environments, Book of abstracts, 2016, ICHA, Florianopolis, Brazil, 28-28. http://icha2016.com/program/

Daniela Marić Pfannkuchen, Ana Baričević; Mirta Smodlaka Tanković, Ingrid Ivančić, Nataša Kužat, Emina Pustijanac, Grozdan Kušpilić, Tihana Novak, Blaženka Gašparović, Martin Pfannkuchen, Exploring the structure of a planktonic ecosystem. Book of abstracts, 2017, International Phycological Society, Szczecin, Poland, 126-126. http://ipc11.intphycsoc.org/doc/IPC%2011%20timetable.pdf

Nataša Kužat, Mirta Smodlaka Tanković, Ana Baričević, Ingrid Ivančić, Emina Pustijanac, Kušpilić Grozdan, Tihana Novak, Blaženka Gašparović, Martin Pfannkuchen, Daniela Marić Pfannkuchen, Interspecific variation in metabolic response to P limitation helps explaining sympatric congeneric species diversity (in the northern Adriatic), Book of abstracts, 2017, International Phycological Society, Szczecin, Poland, 111-111. http://ipc11.intphycsoc.org/doc/IPC%2011%20timetable.pdf

Blaženka Gašparović, Tihana Sesar, Milan Čanković, Zrinka Ljubešić, Enis Hrustić, Zhouyi Zhu, Ruifeng Zhang, Jinzhou Du: How Phytoplankton Membranes Cope With Steep Ionic Strength (Salinity) Gradient? Ocean Sciences Meeting 2016, New Orleans, USA, 21-26 02. 2016. https://agu.confex.com/agu/os16/meetingapp.cgi/Person/238127

Marina Mlakar, Željka Fiket, Vlado Cuculić, Neven Cukrov, Sunčana Geček: Speciation and Distribution of Trace Metals and Organic Matter in Marine Lake as In Situ Laboratory. Ocean Sciences Meeting 2016, New Orleans, USA, 21-26 02. 2016. https://agu.confex.com/agu/os16/meetingapp.cgi/Paper/89673

Tihana Sesar: Phytoplankton cellular lipid response on replete and deplete phosphorous conditions, COST Action ES1408: 1st EUALGAE workshop of algae bioproducts for early stage researchers, Valladolid, Spain, 04. 04. 2016.

Marta Plavšić, Slađana Strmečki: The characterization of polysaccharides in seawater accessed by electrochemical methods. Book of abstracts ( ISSBN 978-84-608-9990-7), 04-07. 09. 2016, Interfaces Against Pollution Conference ( IAP 2016), Llieda, Spain.


Izrađeni diplomski radovi:

Matea Bertanjoli: Promjene sastava lipida diatomeje Chaetoceros pseudocurvisetus ovisno o dostupnosti nutrijenta fosfata

Petra Mlacović: Adaptacija odabranih vrsta morskih dijatomeja na ograničene koncentracije fosfora putem promjene sastava lipida


Terenski rad:

U sklopu projekta proveli smo uzorkovanje morske vode na sjevernom Jadranu, na transektu Rovinj ušće rijeke Po, na dvije postaje 107 i 108 (Slika D6.1.1). Izbor postaja je određen trofičkim statusom same postaje. Postaja 107 je oligotrofna (nedostatak hranjivih soli) dok je postaja mezo- do eutrofna (koncentracija hranjivih soli povoljna do prevelika). Uzorkovane su tri dubine 0, 5 i 10 m. Uzorkovali smo pomoću istraživačkog broda „Vila Velebita“ Instituta Ruđer Bošković. Uzorkovanja su provedena mjesečno, uključujući datume: 16. studenog 2016, 12. prosinca 2016, 27. siječnja 2017, 17. veljače 2017, 28. ožujka 2017, 03. svibnja 2017, 12. lipnja 2017, 12. srpnja 2017, 30. kolovoza 2017 i 04. listopada 2017


Mijesta uzorkovanja ambiomeres

Slika D6.1.1. Mjesta uzorkovanja u sjevernom Jadranskom moru.


Uzorkovanje Vila Velebita ambiomeres

Slika D6.1.2. Uzorkovanje na brodu „Vila Velebita“.


Obrada uzorana na Vili ambiomeres


Članovi projekta AMBIOMERES sudjeluju u sljedećim COST akcijama:

1. 2016 COST Action CA15121 -Advancing marine conservation in the European and contiguous seas(MarCons)

2. 2015 COST Action ES1408 -The European network for algal-bioproducts(EUALGAE)

3. 2016 COST Action CA15217 - Ocean Governance for Sustainability - challenges, options and the role of science.


Kupljena oprema: 



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Glavni istraživač / voditelj
Blaženka Gašparović

Blaženka Gašparović, dr.sc.

znanstveni savjetnik +385 1 456 1148

Ostali suradnici
Vlado Cuculić

Vlado Cuculić, dr. sc.

Viši znanstveni suradnik +385 1 456 1190
Sanja Frka Milosavljević

Sanja Frka Milosavljević, dr.sc.

Viši znanstveni suradnik +385 1 456 1185
Snježana Kazazić

Snježana Kazazić, dr. sc.

znanstvena suradnica +385 1 468 0096
Marina Mlakar

Marina Mlakar, dr.sc.

znanstveni savjetnik u trajnom zvanju +385 1 456 1190
Abra Penezić

Abra Penezić, dr.sc.

asistent +385 1 456 1190
Marta Plavšić - Nemate ovlasti za pregled ovog sadržaja.
Slađana Strmečki Kos

Slađana Strmečki Kos, dr.sc.

znanstveni suradnik +385 1 468 0127
+385 99 6929 861
Petra Vukosav

Petra Vukosav, dr.sc.

znanstveni suradnik (na radnom mjestu višeg asistenta) +385 1 456 1190

Pristupno predavanje

Lokacija: Predavaonica III krilo
Datum: 28.08.2018. Vrijeme: 11:00

Predavač: dr.sc. Ana-Sunčana Smith
Ustanova: Institut Ruđer Bošković

Transport and complexation associated with soft and biological interfaces


Pristupno predavanje

Lokacija: Predavaonica III krilo
Datum: 30.08.2018. Vrijeme: 13:00

Predavač: dr.sc. Ivana Tucaković
Ustanova: Institut Ruđer Bošković

Izazovi u detekciji i analizi - od egzotičnih atoma do radionuklida

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