LMVB image

Laboratory of molecular virology and bacteriology

The lab focuses on molecular analysis of infectious diseases, primarily human papillomaviruses (HPV) and HPV associated cancer

Magdalena Grce

Head

PhD Magdalena Grce

+385 1 456 1110
Fax: +385 1 456 10 10

Phone: +385 1 456 1110

Fax: +385 1 456 1010

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Mission

The mission of the Laboratory of Molecular Virology and Bacteriology is to expand and strengthen knowledge and understanding of the nature of human cancer associated to infectious agents to improve and develop new strategies for the diagnosis, treatment and prevention of this type of cancer.

 
Activities
  • Basic research in molecular pathogenesis of human papillomavirus (HPV)
  • Epidemiological research of HPV at different anatomical sites
  • Epigenetic analysis of HPV associated diseases
  • Research on E6 and E7 oncogene role in HPV associated disease development
  • Department unit for molecular analysis of viral and bacterial infections in humans
  • Department facility for DNA and RNA isolation from different samples using an automatic system EZ1 (Qiagen)
  • The laboratory maintains Croatian bank of nucleic acids (DNA/RNA) isolated from HPV associated cancer and precursors lesions
  • The laboratory maintains cell and bacterial cultures for growth and/or expression of HPV genes
Publications in the last 5 years
  • Brzak BL, Cigić L, Baričević M, Sabol I, Mravak-Stipetić M, Risović D. Different Protocols of Photobiomodulation Therapy of Hyposalivation. Photomed Laser Surg. 2018 Feb;36(2):78-82. doi: 10.1089/pho.2017.4325.
  • Lončar-Brzak B, Klobučar M, Veliki-Dalić I, Sabol I, Kraljević Pavelić S, Krušlin B, Mravak-Stipetić M. Expression of small leucine-rich extracellular matrix proteoglycans biglycan and lumican reveals oral lichen planus malignant potential. Clin Oral Investig. 2018 Mar;22(2):1071-1082. doi: 10.1007/s00784-017-2190-3.
  • Sabol I, Milutin Gašperov N, Matovina M, Božinović K, Grubišić G, Fistonić I, Belci D, Alemany L, Džebro S, Dominis M, Šekerija M, Tous S, de Sanjosé S, Grce M. Cervical HPV type-specific pre-vaccination prevalence and age distribution in Croatia. PLoS One. 2017 Jul 10;12(7):e0180480. doi: 10.1371/journal.pone.0180480. eCollection 2017.
  • Szalmás A, Tomaić V, Basukala O, Massimi P, Mittal S, Kónya J, Banks L. The PTPN14 Tumor Suppressor Is a Degradation Target of Human Papillomavirus E7. J Virol. 2017 Mar 13;91(7). pii: e00057-17. doi: 10.1128/JVI.00057-17.
  • Zine El Abidine A, Tomaić V, Bel Haj Rhouma R, Massimi P, Guizani I, Boubaker S, Ennaifer E, Banks L. A naturally occurring variant of HPV-16 E7 exerts increased transforming activity through acquisition of an additional phospho-acceptor site. Virology. 2017 Jan;500:218-225. doi:10.1016/j.virol.2016.10.023.
  • Kranjec C, Tomaić V, Massimi P, Nicolaides L, Doorbar J, Banks L. The high-risk HPV E6 target scribble (hScrib) is required for HPV E6 expression in cervical tumour-derived cell lines. Papillomavirus Res. 2016 Dec;2:70-77. doi: 10.1016/j.pvr.2016.04.001.
  • Tomaić V. Functional Roles of E6 and E7 Oncoproteins in HPV-Induced Malignancies at Diverse Anatomical Sites. Cancers (Basel). 2016 Oct 19;8(10). pii: E95. Review.
  • Ganti K, Massimi P, Manzo-Merino J, Tomaić V, Pim D, Playford MP, Lizano M, Roberts S, Kranjec C, Doorbar J, Banks L. Interaction of the Human Papillomavirus E6 Oncoprotein with Sorting Nexin 27 Modulates Endocytic Cargo Transport Pathways. PLoS Pathog. 2016 Sep 20;12(9):e1005854. doi:10.1371/journal.ppat.1005854.
  • Petrušić N, Posavac M, Sabol I, Mravak-Stipetić M. The Effect of Tobacco Smoking on Salivation. Acta Stomatol Croat. 2015 Dec;49(4):309-15. doi: 10.15644/asc49/4/6. Gašpar M, Glavina A, Grubišić K, Sabol I, Bušić M, Mravak M; Stipetić. The Oral Cavity State in Renal Transplant Recipients. Acta Stomatol Croat. 2015 Sep;49(3):204-13. doi: 10.15644/asc49/3/2.
  • Sabol I, Smahelova J, Klozar J, Mravak-Stipetic M, Gheit T, Tommasino M, Grce M, Tachezy R. Beta-HPV types in patients with head and neck pathology and in healthy subjects. J Clin Virol. 2016Sep;82:159-65. doi: 10.1016/j.jcv.2016.07.019. Epub 2016 Jul 28
  • Vojtechova Z, Sabol I, Salakova M, Smahelova J, Zavadil J, Turek L, Grega M, Klozar J, Prochazka B, Tachezy R. Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones. BMC Cancer. 2016 Jul 4;16:382. doi: 10.1186/s12885-016-2430-y.
  • Čeović R, Desnica L, Pulanić D, Serventi Seiwerth R, Ilić I, Grce M, Mravak Stipetić M, Klepac Pulanić T, Bilić E, Bilić E, Milošević M, Vrhovac R, Nemet D, Pavletic SZ. High frequency of cutaneous manifestations including vitiligo and alopecia areata in a prospective cohort of patients with chronic graft-vs-host disease. Croat Med J. 2016 Jun 30;57(3):229-38.
  • Jankovic M, Sabol I, Zmak L, Jankovic VK, Jakopovic M, Obrovac M, Ticac B, Bulat LK, Grle SP, Marekovic I, Samarzija M, van Ingen J. Microbiological criteria in non-tuberculous mycobacteria pulmonary disease: a tool for diagnosis and epidemiology. Int J Tuberc Lung Dis. 2016 Jul;20(7):934-40. doi: 10.5588/ijtld.15.0633.
  • Dediol E, Sabol I, Virag M, Grce M, Muller D, Manojlović S. HPV prevalence and p16INKa overexpression in non smoking non drinking oral cavity cancer patients. Oral Dis. 2016 Mar 19. doi:10.1111/odi.12476.
  • Vojtechova Z, Sabol I, Salakova M, Turek L, Grega M, Smahelova J, Vencalek O, Lukesova E, Klozar J,Tachezy R. Analysis of the integration of human papillomaviruses in head and neck tumours in relation to patients' prognosis. Int J Cancer. 2016 Jan 15;138(2):386-95. doi: 10.1002/ijc.29712. Epub 2015 Aug 13.
  • Milutin Gašperov N, Sabol I, Planinić P, Grubišić G, Fistonić I, Ćorušić A, Grce M. Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer. PLoS One. 2015 Jun 9;10(6):e0129452. doi: 10.1371/journal.pone.0129452. eCollection 2015.
  • Zmak L, Obrovac M, Jankovic Makek M, Sabol I, Katalinic-Jankovic V. Isolation of non-tuberculous mycobacteria among tuberculosis patients during a 5-year period in Croatia. Infect Dis (Lond). 2015 Apr;47(4):275-6. doi:10.3109/00365548.2014.987164. Epub 2015 Feb 17.
  • Mravak-Stipetić M, Lončar-Brzak B, Bakale-Hodak I, Sabol I, Seiwerth S, Majstorović M, Grce M. Clinicopathologic correlation of oral lichen planus and oral lichenoid lesions: a preliminary study. Sci World J. 2014;2014:746874. doi: 10.1155/2014/746874. Epub 2014 Oct 29.
  • Milutin Gašperov N, Farkas SA, Nilsson TK, Grce M. Epigenetic activation of immune genes in cervical cancer. Immunol Lett. 2014 Dec;162(2 Pt B):256-7. doi: 10.1016/j.imlet.2014.09.019. Epub 2014 Oct 13.
  • Grce M. [Molecular diagnosis of oral infections]. Acta Med Croatica. 2013 Dec;67(5):425-32. Review [In Croatian].
  • Zhang C, Park JS, Grce M, Hibbitts S, Palefsky JM, Konno R, Smith-McCune KK, Giovannelli L, Chu TY, Picconi MA, Piña-Sánchez P, Settheetham-Ishida W, Coutlée F, De Marco F, Woo YL, Ho WC, Wong MC, Chirenje MZ, Magure T, Moscicki AB, Sabol I, Fiander AN, Chen Z, Chan MC, Cheung TH, Burk RD, Chan PK. Geographical distribution and risk association of human papillomavirus genotype 52-variant lineages. J Infect Dis. 2014 Nov 15;210(10):1600-4. doi: 10.1093/infdis/jiu310. Epub 2014 May 30.
  • Grce M, Mravak-Stipetić M. Human papillomavirus-associated diseases. Clin Dermatol. 2014 Mar-Apr;32(2):253-8. doi: 10.1016/j.clindermatol.2013.10.006. Epub 2013 Oct 30. Review.
  • Drvar DL, Lipozenčić J, Sabol I, Mokos ZB, Ilic I, Grce M. Human papillomavirus status in extragenital nonmelanoma skin cancers. Clin Dermatol. 2014 Mar-Apr;32(2):248-52. doi:10.1016/j.clindermatol.2013.08.009.
  • Hunjak B, Sabol I, Vojnović G, Fistonić I, Erceg AB, Peršić Z, Grce M. Ureaplasma urealyticum and Ureaplasma parvum in women of reproductive age. Arch Gynecol Obstet. 2014 Feb;289(2):407-12.doi: 10.1007/s00404-013-2980-z. Epub 2013 Aug 4.
  • Farkas SA, Milutin-Gašperov N, Grce M, Nilsson TK. Genome-wide DNA methylation assay reveals novel candidate biomarker genes in cervical cancer. Epigenetics. 2013 Nov;8(11):1213-25. doi:10.4161/epi.26346. Epub 2013 Sep 12.
  • Mravak-Stipetić M, Sabol I, Kranjčić J, Knežević M, Grce M. Human papillomavirus in the lesions of the oral mucosa according to topography. PLoS One. 2013 Jul 29;8(7):e69736. doi:10.1371/journal.pone.0069736. Print 2013.
  • Jankovic M, Samarzija M, Sabol I, Jakopovic M, Katalinic Jankovic V, Zmak L, Ticac B, Marusic A, Obrovac M, van Ingen J. Geographical distribution and clinical relevance of non-tuberculous mycobacteria in Croatia. Int J Tuberc Lung Dis. 2013 Jun;17(6):836-41. doi: 10.5588/ijtld.12.0843.

Epigenetic changes in head and neck squamous cell carcinoma

Glavni istraživač / voditelj: Magdalena Grce

This project is a comprehensive interdisciplinary research on the basis of HNC development and potential new approaches for their diagnosis and treatment.

more »
  • Knežević, Marjana - PhD thesis 2017, Masters thesis 2008
  • Hunjak, Blaženka - PhD thesis 2011
  • Obrovac, Mihaela - PhD thesis 2011, Masters thesis 2008
  • Cindrić, Anita - diploma thesis 2012
  • Jerković, Ivana - diploma thesis 2010
  • Bacinger, Ines - diploma thesis 2008
  • Matovina, Mihaela - PhD thesis 2006, Masters thesis 2002
  • Halec, Gordana - diploma thesis 2006
  • Kraljević, Sandra - Masters thesis 2003
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