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Metal-binding biomolecules and health disturbances of freshwater organisms exposed to industrial wastes (METABIOM)

Category
Projekti Hrvatske zaklade za znanost
Project code
IP-2019-04-2636
Finance value
990738
HRK
Start date
Jan 1st 2020
End date
Dec 31st 2023
Status
Active

Metal-binding biomolecules and health disturbances of freshwater organisms exposed to industrial wastes (METABIOM)

Metal-binding biomolecules and health disturbances of freshwater organisms exposed to industrial wastes (METABIOM)

Factory at the Mrežnica River in the vicinity of town Duga Resa.

Project co-directors: dr. Dušica Ivanković and dr. Damir Valić

The aim of this project is to extend the knowledge on the consequences of metal pollution in the freshwater ecosystems usingtwo freshwater bioindicators, fish species northern pike (Esox lucius) and the bivalves from the family Unionidae, from the ecologically important Croatian river Mrežnica, which, in its lower course, is impacted by the waste of several industrial facilities. The information on the river water and sediment contamination with metals will be associated with the information on the metal bioaccumulation and responses of several biomarkers(of general stress, metal exposure, antioxidative capacity and oxidative damage) in the fish liver and bivalve digestive gland, as their main metabolic and detoxification organs, as well as with the information on the fish health status. The project also aims to clarify the fate of metals in fish and bivalve tissues and cells, by application of metallomic approach. By use of three HPLC techniques (size exclusion, anion and cation exchange) combined with HR ICP-MS, metal distribution among cytosolic biomolecules of different sizes and charges in the northern pike liver and bivalve digestive gland will be determined. The metal-binding biomolecules of interest, which could be used as biomarkers of metal exposure or effects, will be further characterized and identified by two mass spectrometry methods (MALDI-TOF-MS and LC-MS/MS). The obtained information will lead to increased understanding of metal functions, detoxification and mechanisms of metal toxicity. Another important aspect of the project will be the assessment of the ecological status of the Mrežnica River and the recognition of the most threatened locations and the most important contaminants, which will help liable authorities to ensure the most effective protection of the water quality and the preservation of the aquatic life of this river.

Other associates

Elisa Blanco González, Full Professor

University of Oviedo, Oviedo, Spain

Emil Gjurčević, Associate Professor

Faculty of Veterinary Medicine, University of Zagreb

Jasna Lajtner, Associate Professor

Faculty of Science, University of Zagreb

Krešimir Matanović, Assistant Professor

Faculty of Veterinary Medicine, University of Zagreb

Krešimira Trgovčić, MSc

Vodovod i kanalizacija d.o.o. Karlovac

María Montes Bayón, Full Professor

University of Oviedo, Oviedo, Spain

Snježana Kužir, Associate Professor

Faculty of Veterinary Medicine, University of Zagreb

Zvjezdana Šoštarić Vulić

Senior technician
Tel:
+385 1 468 0943
Internal phone number:
1435
1939

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