HPLC is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.
General instrument information
0030862, 0308620, 0308621
sitna (< 26.544,56 EUR / 200.000 kn)
Standalone or bound
Varian Inc. (danas Agilent Technologies)
Principle of work
Gradient pumps offer selectable one-, two-, or four-stroke proportioning is available to optimize gradient precision on long, shallow gradients while minimizing gradient delay volume on steep, fast gradients. Reliable results -- Intelligent design, including electronic flow and in-line, high-speed proportioning valves, assures accurate and reproducible results. GLP compliance -- Run-and-error logs maintain a complete audit trail of operating parameters, set points, errors, and faults. Built-in automations -- Up to five methods can be built and stored at the pump. Choose the appropriate method with a single keystroke.
Detailed technical characteristics
Interface Contact closure outputs: Six time-programmable external event relays, and three synchronization signals: READY, START, and FAULT Contact closure inputs: Three synchronization signals: READY, START, and FAULT Bus communication: The 220/230/240 solvent delivery systems communicate with the Star Workstation by means of the IEEE 488 Bus (GPIB) Environment Operates within specifications from 10 °C to 35 °C. Non-operational storage from -20 °C to 65 °C. Operates in relative humidity from 5% to 95%. Dimensions: 29.2 cm (w) x 29.2 cm (h) x 50.2 cm (d) 11.5 in. (w) x 11.5 in. (h) x 19.75 in. (d) Weight: 27.7 Kg (61 lbs.);Technical Data Maximum operating pressure: 6000 psi (410 atmospheres) Flow rate range: 0.01 to 10.0 mL/min in 0.01 mL/min steps between 0.00 and 1.00 mL/min, and 0.1 mL/min steps between 1.0 and 10.0 mL/min Flow precision: 0.3% RSD at 1.0 mL/min MeOH/water Flow accuracy: ± 0.5% at 1.0 mL/min isopropyl alcohol Compositional accuracy: ± 0.5% absolute Compositional precision: ± 0.1% absolute Delay volume: 700 µL + 1 µL/ATM pressure Pressure pulsation: Less than 2% at 150 atmospheres (1 mL/min with 100% isopropyl alchohol) Proportioning modes: 1, 2, or 4-stroke (user selectable) Mixing: High pressure, static or dynamic mixing modes Display: 40 Characters, double-line, back-lit LCD