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Equipment & Techniques

  • Lab space 126 m2;
  • Instruments: Tecan spectrophotometer/fluorometer (Infinite M200), PCR  (Biometra), Biospec Nano (Shimadzu), Bioanalyzator 2100 (Agilent),  ultracentrifuge (Sorval RC28S)
  • Cell culture equipment (laminar flows, wet and dry incubators, cca. 30 cell lines, microscopes), basic and advanced cell culture techniques;
  • Isolation and work with primary fish (rainbow trout) hepatocytes;
  • Equipment for basic molecular biology methods (PCR, qRT-PCR, Nanodrop, cloning, stable and transient transfection)
  • Immunochemical determinations: Western blot and ELISA
  • Enzymatic analysis (activity of the phase I and phase II detoxification enzymes)
  • In vitro and in vivo determinations of protein transport activities (fluorescent or radio labeled substrates, ATPase assay)
  • Cytotoxicity determinations, chronic toxicity test (AlgalTox), genotoxicity tests (Ames test), estrogenicity (YES assay)
  • Small scale zebrafish facility: a room with PP-module (Aquaschwartz) for breeding and experiments with zebrafish adults and embryos (capacity 1200 adults, various sizes of fish aquaria); separate tanks for basic fish stocks (2 independent systems for two certified (AB and WIK) zebrafish strains; capacity 200 adults each); separate nursing tank for embryos with; spawning tanks. Equipment for microinjection of zebrafish embryos: Eppendorf FemtoJet programmable microinjector, external compressor;  WPI M3301 manual micromanipulator, Motic SMZ-171-TLED stereo microscope with digital camera (5 MP) and adapter for fluorescent determinations, incubator (2x) for zebrafish embryos;

Division for Marine and Environmental Research

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