High performance liquid chromatography coupled with mass spectrometry

Category

Farmaceutska industrija

Medicina

Zaštita okoliša

Prehrambena industrija

Kemijska i petrokemijska industrija

Usluge raznih namjena

The system consists of HPLC Agilent 1200 Series and triple quadrupole mass spectrometer Agilent 6410.

Electrospray Ionisation - ESI
Multimode source (ESI i APCI)

Measurements modes:

MS scan
Selective Ion Monitoring (SIM)
MS/MS product ion
MS/MS precursor ion
Neutral gain/loss
Multiple Reaction Monitoring (MRM)

Applicability of the MS/MS system:

Qualitative analysis:

• recording of spectra
• determination of molecular mass
• analysis of fragmentation of molecular ion (product ion)
• determination of precursor of fragment (precursor ion)

Quantitative analysis:

• calibration by use of three standard solutions of analyte and internal standard by MRM technique. Unknown concentration is determined by calibration curve.

Terms of use

According to agreement with Laboratory members, and filling the form.

Price list (per hour)

For RBI employers 40.00 kn
For For outer users, under the RH science system 72.29 kn
For other users 430.50 kn

Room

Laboratory for Carbohydrate, Peptide and Glycopeptide Research
K2 131

Contact

Ivanka Jerić, Ph.D.
e-mail: ijeric@irb.hr
tel. +385 1 4560998
fax. +385 1 4680 195

Lidija Brkljačić, B.Sc.
e-mail: lidija.brkljacic@irb.hr
tel. +385 1 4560998
fax. +385 1 4680 195

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High performance liquid chromatography coupled with mass spectrometry

Institut Ruđer Bošković

Phone:

+385 1 4561 111