Multipoint confocal microscope with a module for laser ablation Opterra Bruker
Contacts
The Opterra Multipoint Scanning Confocal Microscope is based on Bruker’s patented swept-field imaging scanner. This scanner allows high-speed confocal imaging of live cell and small organism preparations at resolutions comparable to conventional point scanners, but with minimal phototoxicty.
The device was bought with funds from the ERC project NewSpindleForce.
General instrument information
Short name
Opterra
Inventory number
44045
Category
kapitalna, odnosno velika (>400.000 EUR)
Instrument type
mikroskop
Analysis type
analiza bioloških uzoraka
Applications
vizualizacija
Standalone or bound
samostalan
Equipment condition
potpuno funkcionalan
Disciplines
Biologija
Year of manufacture
2015
Acquisition date
01.01.2015
Financing body
Europska unija
External link
Characteristics
Model
Opterra
Manufacturer
Bruker
Detailed technical characteristics
Base: Inverted microsope with a motorized 6-position emission filter wheel (fluorescence filters for DAPI, GFP and RFP) • Motorized turret with objectives: 20× (water/glycerol/oil), 100×/1.4 (oil) • Combination galvanometer and piezoelectric crystal scanning (Z-stack) • Excitation options: 405/488/561/640 nm diode lasers, laser power: 100/150/50/100 mW • Fully motorized aperture plate with 7 software-selectable apertures: 3 pinhole settings (30, 45, and 60 μm); 4 slit settings (22, 35, 50, and 70 μm) • Possibility of simultaneous acquisition in three detection channels • Laser ablation: Ablation laser: pulse shorter than 300 fs, wavelength 1030 nm, minimal power 1 W, frequency 10-80 MHz • Precise ablation of areas around 500 nm
Additional equipment
• EMCCD camera: camera pixel size up to 16 µm; image pixel size less than 100 nm; 1000 images/sec with 64 × 64 pixel size; automatic focus • Incubation chamber for live cell imaging, with control and management of CO2, temperature, and humidity.
Purchase price
600000 EUR
Portability
No
Remote work
No
Location
Room
Radione 2
Terms of use
User instructions
Reservations can be made by phone or email with the instrument manager or contact person.
Usage method
korištenje uz pomoć ovlaštene osobe