Time resolved fluorescence (TRF) fluorimeter Edinburgh Inst. FS5

Contacts

+385 1 457 1326

The instrument for stationary and time-resolved fluorescence (TRF) can record the fluorescence spectra of common solutions and solid samples, but the special feature of this instrument is the possibility of time-resolved fluorescence measurements of time-resolved fluorescence solutions with a resolution below 1 ns, which allows accurate and precise characterization of any fluorescent solution.

General instrument information

Short name

TRF fluorimeter

Inventory number

47958

Characteristics

Model

Edinburgh Inst. FS5

Manufacturer

Edinburgh Inst.

Operating and measuring scope

fluorescence 200 nm-1000 nm

Principle of work

Fluorescence spectrophotometry, pulse lasers of various wavelengths with a resolution of <1ns.

Detailed technical characteristics

Light Source - 150 W xenon lamp Optical System - All reflective - Czerny Turner Monochromators (both excitation and emission) must have over 220mm focal length, and have grating turrets accepting up to 2 gratings. - Bandpass from 0* to 30 nm, continuously adjustable via software (*resolution limit of 0.3 nm) - Wavelength accuracy ±0.5 nm - Scan speed 100 nm/s - Sensitivity > 6000:1 (SQRT Method) Excitation Monochromator - Mechanical wavelength coverage: 200 nm-1000 nm - 1200 g/mm grating optimised for 300 nm Filter wheels Fully automatic filter wheels both in excitation and emission path to remove unwanted second and third-order grating effects. Detectors Reference Detector - Calibrated silicon reference detector Transmission Detector - Detector for transmission/absorption measurements must be included to make absorption measurement Emission Monochromator - Mechanical wavelength coverage: 200 nm-1000 nm - 1200 g/mm grating optimised for 500 nm - Automatic filter wheel for second order removal Emission Detector - Time-correlated Single photon counting (TCSPC) photomultiplier in temperature stabilised housing - TCSPC capability, including an electronic card with at least 305 fs/channel bin width, <25 ps electronic jitter and reverse mode automation. - Spectral range: 250 nm -850 nm or broader range Data Acquisition Electronics - Three parallel counter modules for fluorescence, reference and transmission detector. This allows simultaneous correction of excitation intensity. Guaranteed SQRT or FSD Signal to Noise Ratio of at least 6,000:1 for the water Raman signal (excitation at 350 nm, emission at 397 nm, with a 1 second integration time and 5 nm spectral bandwidth). Background signal defined as amplitude at 450nm, without background subtraction and without averaging. Software: for fluorescence software Set of lasers/diodes 340, 405, 450, 560 nm

Additional equipment

Termostates

Purchase price

140000 EUR

Portability

Remote work

Location

Address

Bijenička c. 54

Room

4. krilo, 1 kat, soba 4c

Terms of use

Usage method

samostalno korištenje (kvalificirani/ovlašteni korisnici)