The ALIENA project recognizes the significant ecological and economic impacts of Non-indigenous Species (NIS) through habitat modification and biodiversity loss, particularly in the Adriatic Sea, a hub for fishing, tourism, and maritime traffic. The intensification of these activities increases the risk of NIS introduction. To address this, the project aims to establish a joint monitoring system to protect biodiversity and mitigate socio-economic impacts. Through identifying target NIS, conducting joint monitoring and modeling activities, and developing early warning solutions, the project seeks to enhance marine area management, biodiversity protection, and public health. It also aims to improve shared protocols for NIS detection, monitoring, and management while increasing stakeholder awareness, though a cross-boarder approach. Adopting a cross-border approach for NIS management, including data sharing and harmonized monitoring procedures, the results will enhance knowledge and the efficacy of public policies, particularly for species not yet monitored in some areas.