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Mollusk Larval Shell Ultrastructure and Proteomics: Integrating Analytical Tools

Project type
Znanstveno-istraživački projekti
Programme
The New International Fellowship Mobility Programme for Experienced Researchers in Croatia
Financier
Ministry of Science and Education of the Republic of Croatia
Start date
Oct 1st 2014
End date
Nov 30th 2017
Status
Done
Total cost
37958 EUR
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The aim of his NEWFELPRO project was to explore and describe the fabrication of the shell during the larval development, in selected marine and terrestrial mollusks. To this aim, two approaches are used: ultrastructural and molecular. For the first, larval shells is analyzed according to sophisticated analytical techniques including Infra-Red and Raman spectroscopy, X-ray diffraction, scanning and transmission electron microscopy. The molecular aspects are tackled, by using proteomics and immunohistological techniques, in order to identify the protein assemblage required for early shell mineralization. Particular attention is paid to enzymatic markers. Besides a deepened understanding of the larval shell formation process, this project provides a platform for using molecular markers to study the impact of environmental changes (e.g. Ocean Acidification) on shell biomineralization.

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