Mariculture is a key component of Mediterranean coastal economies, providing sustainable food and ecosystem services while reducing pressure on overexploited wild stocks. However, it is increasingly vulnerable to climate-driven environmental variability. Salinity and temperature variability are major stressors in coastal seas, and the Adriatic is already experiencing rapid warming, seasonal heat extremes, salinisation, and altered stratification driven by freshwater inputs and temperature changes, all affecting mariculture.REANIMAR is an interdisciplinary project developing a mechanistic framework to assess bivalve resilience to salinity–temperature variability in a well-characterised mariculture system. Lim Bay, a major Adriatic mariculture area, combines natural gradients with co-occurring mussels, oysters, and non-indigenous/biofouling species, serving as a model for semi-enclosed, climate-sensitive systems. The project will quantify how environmental gradients and conditions shape bivalve performance, stress responses, and biofouling, translating results into indicators, guidelines, and mitigation tools.REANIMAR will: (1) characterise salinity–temperature gradients and freshwater inputs using high-frequency measurements and larval imaging; (2) assess effects on physiological condition, stress biomarkers, nutritive composition, and shell traits, identifying tolerance ranges; (3) analyse biofouling and non-indigenous species patterns; (4) link valve-gaping behaviour with environmental and biomarker data for early-warning tools; and (5) integrate results into statistical and spatial models defining ecological thresholds and safe operating spaces to guide climate-resilient mariculture.