Dr. Dušica Vujaklija
Laboratory for mass spectrometry and functional proteomics
Ruđer Bošković Institute
Bijenička 54
HR-10000 Zagreb, Croatia
Education
- 1992 PhD obtained at the University of Tokyo, Japan, Advisors: T. Beppu and S. Horinouchi
- 1982 BSc obtained at the Faculty of Food Technology and Biotechnology, University of Zagreb
Projects
• 2019 - CSF project "Young researchers career development project - training new doctoral students"
• 2018 -2022 CSF project "Identifying interactome of paralogous SSB proteins in the multicellular prokaryote, Streptomyces coelicolor" (IP-2018-01-1754)
• 2017 - 2022 ''Bioprospecting of Adriatic Sea" (KK.01.1.1.01), financed by the EU from the European Regional Development Fund. D. Vujaklija is Leader of the WP1, under title: ''Research on biodiversity and genetic resources of marine habitats''
Bilateral projects:
• 2020 - DAAD-RH bilateral project: "New approach to evaluate the binding of ssDNA and paralog SSB proteins from Streptomyces coelicolor"
• 2019 - Bilateral project /Croatian–French Program "Cogito"- Hubert Curien Partnership: „Genetic diversity of marine microbes and phages in selected Adriatic Sea habitats''
Awards and Achievements
- 2020 Plaque of the Croatian Microbiological Society
- 2013 Award of the Ruđer Bošković Institute for the best scientific work in the field
- 2007 For the book "Methods in Molecular Biology" (D. Vujaklija is one of the Editors) RBI obtained the "Josip Juraj Strossmayer" award for the best book in the field of Science in the Republic of Croatia for 2007)
Classes
• Basic principles of genetic engineering, Faculty of Veterinary Medicine, University of Zagreb
Featured Publications
• Bermanec, V., Paradžik, T., Kazazić, S.P.; Venter, C., Hrenović., Vujaklija, D*., Duran, R., Boev, I., B. Boev, Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia, Journal of Hazardous Materials, 2021, 402, 123902, 10 *correspondng author
• Biđin, S., I. Vujaklija, T. Paradžik, A. Bielen, D. Vujaklija* Leitmotif: protein motif scanning 2.0., Bioinformatics, 2020; 36(11): 3566-2020. *correspondng author
• Vujaklija I*, A Bielen*,T Paradžik, S Biđin, P Goldstein and D Vujaklija*, An effective approach for annotation of protein families with low sequence similarity and conserved motifs: identifying GDSL hydrolases across the plant kingdom, BMC Bioinformatics, 2016, 18;17:91 *correspondng author
• Paradzik T, N. Ivic, Z Filic, Babu A. Manjasetty, P Herron, M Luic and D Vujaklija*, Structure-function relationships of two paralogous single-stranded DNA binding proteins from Streptomyces coelicolor: implication of SSB-B in chromosome segregation during sporulation, Nucleic Acids Res., 2013, 1;41(6):3659-72 *correspondng author
• Stefanic Z, D. Vujaklija and M. Luic, Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor, Acta Cryst. D65, 2009, 974-979.
• Mijaković, I., Petranović, D., Maček, B., Čepo, T., Mann, M., Davies, J., Jensen, P. R., Vujaklija, D*. Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucleic Acids Research 2006, 34(5) 1588-1596 *correspondng author
• Petkovič, H., Cullum, J. Hranueli, D., Hunter, I.S., Perić-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D. Long, P.F. Genetics of Streptomyces rimosus, the oxytetracycline producer. Microbiology and Molecular Biology Reviews, 2006, 70 (3), 704-728.
Publications
Books and book chapters
Book chapters
-
Vujaklija, D. & Maček, B. (2012) Detecting Posttranslational Modifications of Bacterial SSB Proteins. U: Keck, J. (ur.) Single-Stranded DNA Binding Proteins. New York (NY), Humana Press ; Springer, str. 205-218 doi:10.1007/978-1-62703-032-8_16.
doi Vujaklija, D. (2007) Plasmid DNA transfre between bacteria from different genera by electroduction. U: Ambriović Ristov, A., Bruvo Mađarić, B., Ćetković, H., Hranilović, D., Herak Bosnar, M., Katušić Hećimović, S., Meštrović Radan, N., Mihaljević, S., Slade, N. & Vujaklija, D. (ur.) Metode u molekularnoj biologiji. Zagreb, Institut Ruđer Bošković, str. 931-933.
Vujaklija, D. (2007) Isolation of genomic DNA from streptomycetes. U: Ambriović Ristov, A., Bruvo Mađarić, B., Ćetković, H., Hranilović, D., Herak Bosnar, M., Katušić Hećimović, S., Meštrović Radan, N., Mihaljević, S., Slade, N. & Vujaklija, D. (ur.) Metode u molekularnoj biologiji. Zagreb, Institut Ruđer Bošković, str. 144-146.
Journal articles
Scientific and review papers
-
Hađina, S., Bruvo Mađarić, B., Kazazić, S., Paradžik, T., Reljić, S., Pinter, L., Huber, Đ. & Vujaklija, D. (2023) Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals. Frontiers in microbiology, 14, 1151107, 12 doi:10.3389/fmicb.2023.1151107.
doiwww.frontiersin.orgfulir.irb.hr -
Šarić, E., Quinn, G., Nalpas, N., Paradžik, T., Kazazić, S., Filić, Ž., Šemanjski, M., Herron, P., Hunter, I., Maček, B. & Vujaklija, D. (2022) Phosphoproteome Dynamics of Streptomyces rimosus during Submerged Growth and Antibiotic Production. mSystems, 7 (5), 1-20 doi:10.1128/msystems.00199-22.
doijournals.asm.orgfulir.irb.hr -
Bermanec, V., Paradžik, T., Kazazić, S., Venter, C., Hrenović, J., Vujaklija, D., Duran, R., Boev, I. & Boev, B. (2021) Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia. Journal of hazardous materials, 402, 123902, 10 doi:10.1016/j.jhazmat.2020.123437.
doi -
Biđin, S., Vujaklija, I., Paradžik, T., Bielen, A. & Vujaklija, D. (2020) Leitmotif: protein motif scanning 2.0. Bioinformatics, 36 (11), 3566-3567 doi:10.1093/bioinformatics/btaa133.
doi -
Terra, L., Dyson, P., Hitchings, M., Thomas, L., Abdelhameed, A., Banat, I., Gazze, S., Vujaklija, D., Facey, P., Francis, L. & Quinn, G. (2018) A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens. Frontiers in microbiology, 9 (OCT), 2458, 13 doi:10.3389/fmicb.2018.02458.
doiwww.frontiersin.org -
Đermić, E., Zahradka, D., Vujaklija, D., Ivanković, S. & Đermić, D. (2017) 3'-terminated Overhangs Regulate DNA Double-Strand Break Processing in Escherichia coli. G3-Genes Genomes Genetics, 7 (9), 3091-3102 doi:10.1534/g3.117.043521.
doi -
Ivanković, S., Vujaklija, D. & Đermić, D. (2017) Nucleolytic degradation of 3′-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli. DNA repair, 57, 56-65 doi:10.1016/j.dnarep.2017.06.024.
doifulir.irb.hr -
Paradžik, T., Filić, Ž. & Vujaklija, D. (2016) Variations in amino acid composition in bacterial single stranded DNA–binding proteins correlate with GC content. Periodicum biologorum, 118 (4), 385-397 doi:10.18054/pb.v118i4.4847.
doihrcak.srce.hr -
Vujaklija, I., Bielen, A., Paradžik, T., Biđin, S., Goldstein, P. & Vujaklija, D. (2016) An effective approach for annotation of protein families with low sequence similarity and conserved motifs : identifying GDSL hydrolases across the plant kingdom. BMC bioinformatics, 17, 91-1 doi:10.1186/s12859-016-0919-7.
doi -
Abella, J., Bielen, A., Huang, L., Delmont, T., Vujaklija, D., Duran, R. & Cagnon, C. (2015) Integron diversity in marine environments. Environmental science and pollution research international, 22 (20), 15360-15369 doi:10.1007/s11356-015-5085-3.
doi -
Duran, R., Bielen, A., Paradžik, T., Gassie, C., Pustijanac, E., Cagnon, C., Hamer, B. & Vujaklija, D. (2015) Exploring Actinobacteria assemblages in coastal marine sediments under contrasted human influences in the West Istria Sea, Croatia. Environmental science and pollution research international, 22 (20), 15215-15229 doi:10.1007/s11356-015-4240-1.
doi -
Bielen, A., Teparić, R., Vujaklija, D. & Mrša, V. (2014) Microbial anchoring systems for cell-surface display of lipolytic enzymes. Food technology and biotechnology, 52 (1), 16-34.
hrcak.srce.hrfulir.irb.hr -
Paradžik, T., Ivić, N., Filić, Ž., Manjasetty, B., Herron, P., Luić, M. & Vujaklija, D. (2013) Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation. Nucleic acids research, 41 (6), 3659-3672 doi:10.1093/nar/gkt050.
doi -
Leščić Ašler, I., Pigac, J., Vujaklija, D., Luić, M. & Štefanić, Z. (2011) Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin. Acta crystallographica. Section F, Structural biology and crystallization communications, 67 (11), 1378-1381 doi:10.1107/S1744309111032222.
doi -
Goldstein, P., Žučko, J., Vujaklija, D., Kriško, A., Hranueli, D., Long, P., Etchebest, C., Basrak, B. & Cullum, J. (2009) Clustering of protein domains for functional and evolutionary studies. BMC bioinformatics, 10, 335, 11 doi:10.1186/1471-2105-10-335.
doibmcbioinformatics.biomedcentral.com -
Štefanić, Z., Vujaklija, D. & Luić, M. (2009) Crystal structure of the single-stranded DNA binding protein from Streptomyces coelicolor. Acta crystallographica. Section D, Biological crystallography, 65 (9), 974-979 doi:10.1107/S0907444909023634.
doi Bielen, A., Ćetković, H., Long, P., Schwab, H., Abramić, M. & Vujaklija, D. (2009) The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity.. Biochimie, 91 (3), 390-400.
-
Castaldo, G., Žučko, J., Heidelberger, S., Vujaklija, D., Hranueli, D., Wattana-Amorn, P., Crump, P., Crosby, J. & Long, F. (2008) Proposed arrangement of proteins forming a bacterial type II polyketide synthase. Chemistry & biology, 15 (11), 1156-1165 doi:10.1016/j.chembiol.2008.09.010.
doi -
Salopek-Sondi, B., Vukelić, B., Špoljarić, J., Šimaga, Š., Vujaklija, D., Makarević, J., Jajčanin, N. & Abramić, M. (2008) Functional tyrosine residue in the active center of human dipeptidyl peptidase III. Biological chemistry, 389 (2), 163-167 doi:10.1515/BC.2008.021.
doi -
Štefanić, Z., Vujaklija, D., Andrišić, L., Mikleušević, G., Andrejašič, M., Turk, D. & Luić, M. (2007) Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein. Croatica chemica acta, 80 (1), 35-39. (https://www.bib.irb.hr/300532).
fulir.irb.hr -
Petković, H., Cullum, J., Hranueli, D., Hunter, S., Perić-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D. & Long, F. (2006) Genetics of Streptomyces rimosus, the Oxytetracycline Producer. Microbiology and Molecular Biology Reviews, 70 (3), 704-728 doi:10.1128/MMBR.00004-06.
doi Mijaković, I., Petranović, D., Maček, B., Čepo, T., Mann, M., Davies, J., Jensen, P. & Vujaklija, D. (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. NUCLEIC ACIDS RESEARCH, 34 (5), 1588-1596.
-
Orhanović, S., Bučević-Popović, V., Pavela-Vrančič, M., Vujaklija, D. & Gamulin, V. (2006) Effect of a Thr81→ Ala Mutation at the Subunit Effect of a T81A mutation at the subunit interface on catalytic properties of alkaline phosphatase from Escherichia coli. International journal of biological macromolecules, 40 (1), 54-58 doi:10.1016/j.ijbiomac.2006.06.008.
doi Ishiyama, D., Vujaklija, D. & Davies, J. (2004) Novel Pathway of Salycilate Degradation by Streptomyces sp. Strain WA46. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70 (3), 1297-1306.
Vujaklija, D., Abramić, M., Leščić, I., Maršić, T. & Pigac, J. (2003) Streptomyces rimosus GDS(L) lipase: production, heterologous overexpression and structure-stability relationship. Food Technology and Biotechnology, 41 (1), 89-94.
Vujaklija, D., Schroeder, W., Abramić, M., Zou, P., Leščić, I., Franke, P. & Pigac, J. (2002) A novel streptomycete lipase: cloning, sequencing and high-level expression of the Streptomyces rimosus GDS(L)-lipase gene. Archives of Microbiology, 178 (2), 124-130.
-
Ahel, I., Vujaklija, D., Mikoč, A. & Gamulin, V. (2002) Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter. FEMS microbiology letters, 209 (1), 129-133 doi:10.1111/j.1574-6968.2002.tb11121.x.
doi Bakal, C., Vujaklija, D. & Davies, J. (2001) Immunochemical Identification of a Stat3 Analogue in Streptomycetes. Food Technology and Biotechnology, 39 (4), 313-317.
Gregorović, G. & Vujaklija, D. (2001) High Efficiency of Direct Plasmid Transfer between two Streptomyces spp., and between Streptomyces spp. and E. coli. Food Technology and Biotechnology, 39 (1), 49-53.
-
Mikoč, A., Vujaklija, D. & Gamulin, V. (1997) The recA gene from Streptomyces rimosus R6: sequence and expression in Escherichia coli. Research in microbiology, 148 (5), 397-403 doi:10.1016/S0923-2508(97)83870-3.
doi -
Vujaklija, D., Žafran, J., Mikoč, A. & Gamulin, V. (1996) Direct detection of extracellular deoxyribonuclease in different strains of Streptomyces rimosus. Food technology and biotechnology, 34 (2-3), 71-76.
www.ftb.com.hr -
Vujaklija, D. & Davies, J. (1995) Direct transfer of plasmid DNA between Streptomyces spp. and Echerichia coli by electroduction. Journal of antibiotics, 48 (7), 635-637 doi:10.7164/antibiotics.48.635.
doi -
Waters, B., Vujaklija, D., Gold, M. & Davies, J. (1994) Protein tyrosine phosphorylation in Streptomycetes. FEMS microbiology letters, 120 (1/2), 187-190 doi:10.1111/j.1574-6968.1994.tb07028.x.
doi -
Vujaklija, D., Horinouchi, S. & Beppu, T. (1993) Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. Journal of bacteriology, 175 (9), 2652-2661 doi:10.1128/jb.175.9.2652-2661.1993.
doi -
Vujaklija, D., Ueda, K., Hong, S., Beppu, T. & Horinouchi, S. (1991) Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus.. MGG. Molecular & general genetics, 229 (1), 119-128 doi:10.1007/BF00264220.
doi -
Pigac, J., Vujaklija, D., Toman, Z., Gamulin, V. & Schrempf, H. (1988) Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces. Plasmid, 19 (3), 222-230 doi:10.1016/0147-619X(88)90040-6.
doi
Conference proceedings papers
Scientific conference proceedings papers
Durmiši, E., Vujaklija, D., Bielen, A., Duran, R. & Hamer, B. (2013) Physicochemical and ecotoxicological evaluation of the Rovinj costal area sediments, northern Adriatic, Croatia. U: de Lange, G., Gačić, M., Romaña, A., da Costa, M., Boero, F., Tura, C. & Sala, E. (ur.)Rapport Commission International pour l'exploration scientifique de la Mer Mediterranee.
Membership in professional associations / societies
- Croatian Genetic Society (member of the Board of Directors since March 17, 2009 -, vice president of CGS from 2015 - 2019)
- Croatian Society for Biochemistry and Molecular Biology (member)
- Croatian Microbiological Society (President of the Bacteriological Section from 2012-2020, member of the CMS Committee for the Annual Awards to young scientist since 2012; member of the CMS Conference Committee, since 2019 and member of the CMS Publishing Committee, since 2015)
- Croatian Society for Mathematical and Theoretical Biology (member since the founding of the Society)
- European Environmental Mutagen Society (member)