MAIN RESEARCH TOPICS

• Investigation of the development and spread of antimicrobial resistance in aquatic and terrestrial ecosystems, including engineered systems such as wastewater treatment plants
• Research on microbial and enzymatic transformations of organic environmental pollutants

SCIENTIFIC COLLABORATIONS

Projects:

• ARES (2024 – 2027)  ̶  Monitoring antimicrobial resistance through groundwater analysis, Croatian Science Foundation project: resistome, microbiome, mobilome, qPCR, 16S rRNA amplicon sequencing, isolation and WGS of pathogens, plasmid transfer

PI: Dr. Nikolina Udiković Kolić

Institutions:

• Croatian Institute of Public Health, Zagreb, Croatia
• Teaching Institute of Public Health "Dr. Andrija Štampar", Zagreb, Croatia
• University Hospital for Infectious Diseases "Dr. Fran Mihaljević", Zagreb, Croatia
• Faculty of Science, Split, Croatia
• University of Shizuoka, Japan
• Austrian Institute of Technology, Vienna, Austria
• Technical University of Denmark
• University of Helsinki, Finland
• Julius Kuehn Institute, Braunschweig, Germany

EQUIPMENT

• Equipment for aseptic cultivation and handling of microbial cultures: sterile cabinets, temperature-controlled shakers and incubators, centrifuges, spectrophotometers, autoclaves, freezers (-20°C, -80°C), light microscope
• Equipment for DNA and RNA manipulation: PCR cabinet, refrigerated centrifuges, PCR devices, electrophoresis equipment with power supplies, transilluminator, electroporator, fluorimeter, ultracentrifuge
• Real-Time PCR devices (Applied Biosystems and Biorad) – jointly owned by the Division for Marine and Environmental Research and the Division of Molecular Biology
• MultiVac 300-MB-T – water membrane filtration system with three positions
• Bioreactor Jupiter 2.0 (Solaris Biotechnology), total volume 2 L
• HPLC system Varian ProStar with ProStar 230 pump, UV-DAD detector, and autosampler

SERVICES

Quantification of antimicrobial resistance genes in environmental samples (water, soil) using quantitative PCR method

POSTGRADUATE TEACHING AND POPULARISATION OF SCIENCE

• University of Osijek, doctoral study in Nature and Environmental Protection, courses "Biotechnology in Environmental Protection" (Dr. N. Udiković Kolić, 2013-2024)
• Popularisation: IRB Open Days, participation in radio and TV programs, STEM Popular Podcast, S1E15 Biology module (N. Udiković Kolić)