Skip to main content

ProNetMel - New Protein Networks for Novel Therapeutic Avenues in Human Melanoma

Principal investigator

Category
Projekti Hrvatske zaklade za znanost
Project code
IP-2013-11-1615
Finance value
996.500,00
HRK
Start date
Sep 1st 2014
End date
Aug 31st 2018
Status
Done
ProNetMel - New Protein Networks for Novel Therapeutic Avenues in Human Melanoma

Metastatic melanoma represents a major clinical problem due to rising incidence and resistance to available therapies. Therefore, there is a necessity for developing novel molecular approaches to treat this disease. Unlike other tumors, in melanoma p53 is relatively rarely mutated. However, overexpressed p53 fails to function as a tumor suppressor and tumor cells continue to proliferate and spread. This suggests that regulation of p53 activity depends on the interplay between p53 and its binding partners. Based on current state of knowledge, we hypothesize that p53 function in malignant melanoma might be altered through the interactions with p53 small molecular weight and p73 isoforms, nm23/NDPK and Gli families of proteins. Thus we will search for p53 protein binding partners in melanoma. The interactions identified by mass spectrometry will be further confirmed using microarray-based approaches and co-immunoprecipitation techniques. Furthermore, the expression profile of genes/proteins of interest in panel of melanoma cell lines and melanoma tissue samples will be investigated. The potential impact of the identified p53 interacting partners on its transcriptional activity and the stimulation of apoptosis will be determined. The depletion of partners using siRNA technology would confirm the involvement of selected proteins on p53 activity. The subcellular localization of the proteins in complex will be revealed by advanced fluorescent microscopy methods on state-of-the-art instrumentation what would give us the insight on dynamics of their interactions. The proposed research could provide a possible explanation why p53 function in melanoma is abrogated. The aim of this project is to determine the complexity of p53 interactions and their role in the onset and progression of human melanoma and offer new, innovative solutions in diagnosis, prognosis and therapy.

Other associates

Maja Herak Bosnar, dr.sc.

Senior scientist
Tel:
+385 1 456 0996
Interni broj:
1752

Maja Jazvinšćak Jembrek, dr.sc.

M

J

Senior research associate
Tel:
+385 1 456 0997
Interni broj:
1753

Arijana Zorić

A

Z

Tel:
+385 1 456 0997
Interni broj:
1753

Anđela Horvat, dr.sc.

A

H

Research associate
Tel:
+385 1 456 0997
Interni broj:
1752
1754

Nikolina Hanžić

N

H

Interni broj:
1754

Martina Pehar

M

P

Technician
Tel:
+385 1 456 1113
Interni broj:
1513
1754

Sonja Levanat

S

L

Tel:
+385 1 457 1267
Interni broj:
1551

Vesna Musani, dr.sc.

Research associate
Tel:
+385 1 457 1292
Interni broj:
1849

Maja Sabol, dr.sc.

Research associate
Tel:
+385 1 456 0997
Interni broj:
1753

Petar Ozretić, dr.sc.

Research associate
Tel:
+385 1 457 1292
Interni broj:
1849

Diana Trnski, dr.sc.

Postdoctoral researcher
Interni broj:
1849
1512

Robert Belužić, dr.sc.

R

B

Research associate
Interni broj:
1606

Collaborators:

Ivan Milas, MD, PhD

Prof. Božena Šarčević, MD, PhD

This site uses cookies.

Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

  • Necessary cookies enable core functionality. The website cannot function properly without these cookies, and can only be disabled by changing your browser preferences.