This research and development project was funded under the call “Strengthening Research, Development and Innovation Capacities” (IRI) from the European Regional Development Fund.

This research and development project was funded under the call “Strengthening Research, Development and Innovation Capacities” (IRI) from the European Regional Development Fund.

The possibility of synthesis of reagents for sample preparation for MS analysis will be investigated, which would enable original "all-in-one" quantitative and qualitative analysis of proteins for the needs of industry and biomedical sciences.

The Hedgehog signaling pathway has been implicated in development of various tumors, but the exact roles and molecular mechanisms are still not understood. The focus of this research are the tumors harboring BRAF or NRAS mutations, and their differential response to Hedgehog pathway inhibition. Both BRAF and NRAS non-canonically activate the three GLI proteins, but the activity and interplay of this interaction is still unclear. With this project we propose to determine the exact transcriptional targets of GLI1, GLI2 and GLI3 proteins in melanoma with different genetic backgrounds, either harboring a BRAF mutation, a NRAS mutation, or no mutation in these two genes.

The main goal of this project is to investigate MSI and EMAST microsatellite instability in sporadic colon cancer and to correlate the obtained results with clinico-pathological characteristics of tumors and patients.