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High performance liquid chromatography coupled with mass spectrometry

Category
  • Farmaceutska industrija
  • Medicina
  • Zaštita okoliša
  • Prehrambena industrija
  • Kemijska i petrokemijska industrija
  • Usluge raznih namjena

The system consists of HPLC Agilent 1200 Series and triple quadrupole mass spectrometer Agilent 6410.

 

  •  Electrospray Ionisation - ESI
  •  Multimode source (ESI i APCI)

 Measurements modes:

  • MS scan
  • Selective Ion Monitoring (SIM)
  • MS/MS product ion
  • MS/MS precursor ion
  • Neutral gain/loss
  • Multiple Reaction Monitoring (MRM)

 Applicability of the MS/MS system:

 Qualitative analysis:

 • recording of spectra
 • determination of molecular mass
 • analysis of fragmentation of molecular ion (product ion)
 • determination of precursor of fragment (precursor ion)

Quantitative analysis:

 • calibration by use of three standard solutions of analyte and internal standard by MRM technique. Unknown concentration is determined by calibration curve.

Terms of use

According to agreement with Laboratory members, and filling the form.

Price list (per hour)

For RBI employers   40.00 kn
For For outer users, under the RH science system   72.29 kn
For other users   430.50 kn

Room

Laboratory for Carbohydrate, Peptide and Glycopeptide Research
K2 131
 
Contact

Ivanka Jerić, Ph.D.
e-mail: ijeric@irb.hr
tel. +385 1 4560998
fax. +385 1 4680 195

Lidija Brkljačić, B.Sc.
e-mail: lidija.brkljacic@irb.hr
tel. +385 1 4560998
fax. +385 1 4680 195

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