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Uređaj fluorimetar i luminometar se koristi za mjerenje fluorescencije i luminiscencije u mikrotitarskim pločama s 1-384 bunarića. Uređaj je spojen na kompjuter s pripadajućim software-om. Sadrži slijedeće filtere: Ex355/Em450nm, Ex485/Em538nm i Ex530/Em590nm. Uređaj je moguće nadograditi s dodatnim parovima filtera.

General instrument information

Skraćeni naziv instrumenta
Fluorimetar/luminometar
Uža područja primjene
Biomedicina i zdravstvo, prirodne znanosti
Namjena instrumenta
mjerenje fluorescencije i luminiscencije
Kategorija
sitna
Vrsta instrumenta
spektrometar
Vrsta analize
biološka/DNA analiza
Samostalan/vezan
Samostalan instrument
Tijelo koje je financiralo nabavku opreme
Ministarstvo znanosti i obrazovanja RH (MZO)
Vanjski link za kapitalnu opremu (sestar.irb.hr)

Characteristics

Model
FLUOROSKAN ASCENT FL
Mrežna URL adresa instrumenta na stranicama proizvođača
Proizvođač
Thermo Scientific
Mrežna URL adresa proizvođača
Radno i mjerno područje
Ex355/Em460nm, Ex485nm/Em538nm i Ex530/Em590nm
Princip rada i mjerna tehnika
Uređaj mjeri fluorescenciju i luminiscenciju u mikrotitarskim pločama s 1-384 bunarića. Uređaj je spojen na kompjuter s pripadajućim software-om. Sadrži slijedeće filtere: Ex355/Em450nm, Ex485/Em538nm i Ex530/Em590nm. Uređaj je moguće nadograditi s dodatnim parovima filtera.
Detaljne tehničke karakteristike
Dynamic Range :
Fluorometry: >6 decades
Luminometry: >9 decades over whole gain setting area

Excitation Filters :
Up to eight filters in the excitation filter wheel. 355 and 485nm filters included as standard. Other filters available upon request.

Light Source : Quartz-halogen Lamp

Detector : Photomultiplier Tube

User Interface : Requires, but does not include, a personal computer

Shaking : Orbital shaker

Measurement Speed : 15 seconds, 96-well plate

Excitation Wavelength Range :
Excitation: 320- 700nm; Emission: 360 - 670nm

Incubator : From ambient +3°C to 45°C, at ambient 25°C

Sensitivity :
Fluorometry: 2 fmol fluorescein/well in a black 96-well plate
Luminometry: 40 amol ATP/well using flash reaction, white 384-plate

Emission Filters :
Up to six filters in the emission filter wheel. 460 and 538nm filters included as standard. Other filters available upon request.

Weight : Basic unit: 21kg (46 lbs.)

Computer Interface : Serial RS-232C port

Plate Types : 1- to 384-well plates
Nabavna cijena
150.000

Location

Zavod
Laboratorij
Grad
Zagreb
Adresa
Bijenčka 54
Krilo/Kat/Soba
V. krilo/Soba 30

Terms of use

Popis ovlaštenih korisnika
dr.sc. Silva Katušić Hećimović
Upute za korisnike
Uzorke pripremiti u mikrotitarskm pločama s 1-384 bunarića.
Način korištenja instrumenta
samostalni rad - ovlašteni korisnici

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