Infectious diseases are predicted to rise in the next year due to antibiotic resistance. As a result, there is a pressing need for disruptive technologies to tackle this medical challenge. The EU-funded PEST-BIN project bridges academia and industry to deliver new diagnostics tools that can detect infection with the accuracy and speed required in a clinical setting. Proteomics analysis with the help of artificial intelligence will provide insight into the mechanisms of bacterial infection and unveil new targets for antibiotic development. Moreover, partners will employ nanotechnology loaded with antibiotics to tackle bacterial biofilms, an inherently difficult structure to penetrate with drugs.