As part of this project, we aim to investigate the interaction between Keap1 and actin in more detail using a synergistic experimental and computational approach, with a particular focus on the role of Keap1 in actin cytoskeleton regulation. Specifically, our objectives are to examine how reduced Keap1 levels affect actin cytoskeleton organization and polymerization, as well as tumor cell migration, to identify the potential domains of Keap1 and actin essential for their interaction, and to determine which components of the Nrf2–Keap1 complex contribute to changes in the actin cytoskeleton and focal adhesions.