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  1. Ruđer Bošković Institute
  2. Divisions
  3. Division of Molecular Medicine
  4. Laboratory of Experimental Thera...
  5. Use of Flow Cytometer BD FACSymp...
  6. In vitro Testing of Antitumor Ac...

In vitro Testing of Antitumor Activity of Newly Synthesized Compounds

The Laboratory for Experimental Therapy offers testing of the antitumor activity of newly synthesized and natural compounds.

  • Sample preparation: Compounds should be delivered dissolved in water or in an appropriate organic solvent (preferably DMSO) at a concentration of 0.04 mol/L (if dissolved in DMSO).
  • Cell lines: Initial testing is performed on three human tumor cell lines. If a compound shows activity, further testing on a wider panel of tumor and/or non-tumor cell lines can be arranged upon agreement.
  • Assay: Cell metabolic activity is measured using the MTT assay. Results are provided in graphical form and as GI50 values (growth inhibition 50%).
  • Pricing: The cost of testing per compound is determined upon request, depending on the number of compounds submitted.

For more information, please contact us.