Laboratory for mass spectrometry

Laboratory for mass spectrometry

Welcome to web pages of Laboratory for chemical kinetics and atmospheric chemistry.

Saša Kazazić

Head

PhD Saša Kazazić

+385 1 456 1077
Fax: +385 1 468 0245
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Area of special concern of the Laboratory members is and will be general basic research with emphasis on the study of biologically active molecules, design, synthesis and properties of organometallic complexes of biological and pharmacological significance and those that can be applied in organic synthesis, catalysis or as chromogenic and fluorescent chemosensors.
 We plan to measure and monitor those relevant components of the atmosphere (reactive oxygen species and reactive nitrogen species) that will allow us to study the oxidation effects of the atmosphere on the living organisms and materials.

Special attention will be oriented to macromolecules – to structural analysis of peptides and proteins in complex biological mixtures. Experiments with state-of-art mass spectrometry techniques is unavoidable in achieving this goal. Thus the new sophisticated mass spectrometers which have been installed in our Laboratory this year will be used: Amazon ETD, Bruker Daltonik (LC-MS) and MALDI TOF Biotyper, Bruker Daltonik. To improve the fundamental understanding of reactivity, (biological) activity and mechanism of other classes of molecules UV photoelectron spectroscopy (PES), electronic (UV-Vis) spectrophotometry together with resident techniques including IR, NMR, EPR and X-Ray diffraction will be used. Experimental studies, combined with computational investigations provide a conceptual framework and an efficient means to obtain direct insight into reactivity patterns, the importance of electronic structure and the nature of intermediates.

One category of mass spectrometry activities will be focused towards a fee-based routine service program for external users including qualitative protein identification and the identification and characterization of microorganisms

Laboratory instrumentation

Instruments available for the work on our research projects:

  • MALDI TOF mass spectrometer system for whole cell mass spectrometry
  • nano-LC-MS/MS system for protein primary structure research, PTM discovery and proteomics
  • Photoelectron Spectrometer UVG 3
  • uv-vis spectrophotometer
  • different kind of monitors for air pollution measurements

Some of the existing equipment is obviously old (e.g. photoelectron spectrometer) but is still performing satisfactorily and is being used to produce high quality research work as evidenced by the publications.

Tabular view of instruments available in laboratory:

     
MALDI-TOF mass spectrometer Bruker Microflex

MALDI-TOF mass spectrometer Bruker Microflex

 

MALDI-TOF mass spectrometer
system for whole cell mass spectrometry

Manufacturer an model: Bruker Daltonik Microflex series
Year of production: 2011.

     
 
Mass spectrometer Bruker amaZon and Dionex HPLC

Mass spectrometer Bruker amaZon and Dionex HPLC

 

Nano LC/MS system
system for protein primary structure research, PTM discovery and proteomics

Manufacturers an models: Bruker Daltonik amaZon i Dionex HPLC. Godina proizvodnje
Year of production: 2011.

     
Photoelectron spectrometer Vacuum Generators UVG 3

Photoelectron spectrometer Vacuum Generators UVG 3

 

Photoelectron Spectrometer UVG 3
Photoelectron Spectrometer

Manufacturer an model: Vacuum Generators UVG 3
Year of production: 1973.

     
UV-VIS spectrophotometer Agilent Agilent G1103A

UV-VIS spectrophotometer Agilent Agilent G1103A

 

UV-VIS spektrophotometer Agilent G1103A
UV-VIS spectrophotometer

Manufacturer an model: Agilent G1103A
Year of production: 2001.

     
Air pollution monitors

Air pollution monitors

 

Different kind of monitors for air pollution measurements

Manufacturers an models:
Monitors: Ansyco O341M, Ansyco AC31M, Environics ozone analyzer series 300
Data logers: DataTaker DT50 Series3, DataTaker 5, Campbell Scientific MicroLogger 21X
Year of production: varying from 1990 to 2005.

     

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