Dr. Iva Tolić
Ruđer Bošković Institute
Bijenička 54
HR-10000 Zagreb
Education
- 2002: PhD in biology, University of Zagreb, Croatia
- 1999 – 2001: PhD work with Prof. Ning Wang, Harvard School of Public Health, Boston, MA, USA
- 1997 – 1999: Graduate studies in molecular biology, Research assistant in the group of Prof. Nenad Trinajstić, Ruđer Bošković Institute and University of Zagreb, Croatia
- 1992 – 1996: Diploma in molecular biology, University of Zagreb, Croatia
Projects
- 2020 – 2026: ERC Synergy Grant with Geert Kops (Hubrecht Institute – KNAW, Netherlands), Angelika Amon (MIT, USA) and Nenad Pavin (University of Zagreb, Croatia). Topic: Molecular origins of aneuploidies in healthy and diseased human tissues (Aneuploidy). Total amount granted: 10,000,000 EUR.
- 2020 – 2024: Grant from the Croatian Science Foundation (HRZZ), together with Nenad Pavin. Topic: Mechanisms of microtubule bundle formation required for spindle maturation. Total amount granted: 1,000,000 HRK (≈130,000 EUR).
- 2019 – 2022: Grant from the Croatian Science Foundation (HRZZ), together with Marin Barišić, Cooperation Programme with Croatian Scientists in Diaspora "RESEARCH COOPERABILITY". Topic: Mechanisms of microtubule bundle formation required for spindle maturation. Total amount granted: 2,200,000 HRK (≈295,000 EUR).
- 2019 – 2022: European Structural and Investment Funds (ESIF) within the Operational Programme Competitiveness and Cohesion (OPCC) 2014 – 2020. Topic: Innovative microscopy protocols for interdisciplinary research in biomedicine. Total amount granted: 5,471,441 HRK (≈735,000 EUR).
- 2015 – 2020: ERC Consolidator Grant. Topic: A new class of microtubules in the spindle exerting forces on kinetochores. Total amount granted: 2,150,000 EUR.
- 2015 – 2019: Research grant from the Croatian Science Foundation (HRZZ). Topic: Oscillatory dynamics of the cytoskeleton. Total amount granted: 1,000,000 HRK (≈130,000 EUR).
- 2015–2016: Grant from the European Social Fund (ESF), together with Igor Weber (PI), Ruđer Bošković Institute, Zagreb, Croatia. Topic: Cytoskeletal dynamics and spindle assembly during cell division. Total amount granted: 1,560,000 HRK (≈210,000 EUR).
- 2013 – 2016: Research grant from the German Research Foundation (DFG) together with Nenad Pavin, University of Zagreb, Croatia. Topic: Kinetochore oscillations in mitotic metaphase. Total amount granted: 256,000 EUR.
- 2013 – 2015: Research grant from the Unity Through Knowledge Fund (UKF) together with Nenad Pavin (PI), University of Zagreb, Croatia. Topic: The role of microtubule pivoting in formation of complex structures such as microtubule bundles and mitotic spindles. Total cost of the project: 1,110,000 HRK (≈150,000 EUR).
- 2012 – 2015: Research grant from DFG under the Priority Programme SPP 1384: Mechanisms of Genome Haploidization. Topic: Homologous chromosome pairing in Schizosaccharomyces pombe. Total amount granted: 304,400 EUR.
- 2010 – 2014: Research grant from the Human Frontier Science Program (HFSP), with Juraj Gregan (PI, University of Vienna, Austria) and Daniela Cimini (Virginia Tech, USA). Topic: Molecular architecture and mechanical properties of the kinetochore: a biophysical approach. Total amount granted: 1,000,000 $.
- 2009 – 2012: Research grant from DFG under the Priority Programme SPP 1384: Mechanisms of Genome Haploidization. Topic: Homologous chromosome pairing in Schizosaccharomyces pombe. Total amount granted: 252,600 EUR.
- 2009 – 2012: Research grant from DFG together with Stefan Diez, MPI-CBG, Germany. Topic: In vitro reconstitution of motor-driven nuclear oscillations. Total amount granted: 304,800 EUR.
- 2008 – 2012: Research grant from DFG. Topic: Meiotic nuclear oscillations in Schizosaccharomyces pombe. Total amount granted: 246,600 EUR.
- 2007 – 2011: Research grant from DFG. Topic: Mechanisms of mitotic spindle alignment by cytoplasmic microtubules and cell shape in Schizosaccharomyces pombe. Total amount granted: 242,600 EUR.
Awards and Achievements
- Member of Academia Europaea, since 2022
- EMBO member, since 2018
- Annual award of the Ruđer Bošković Institute for 8 scientific papers, since 2017
- Outstanding Paper Award of the European Microscopy Society in the category Life Sciences, “The mitotic spindle is chiral due to torques within microtubule bundles”, M. Novak, B. Polak, J. Simunić, Z. Boban, B. Kuzmić, A. Thomae, I.M. Tolić, N. Pavin, Nat Commun 2018
- Ignaz L. Lieben Award of the Austrian Academy of Sciences, 2017
- Award of the Croatian Society of Biochemistry and Molecular Biology for an outstanding contribution to the development of molecular life sciences, 2017
- Award "Didak" for science, 2017
- Croatian Women of Influence Award, 2016
- National Science Award of the Republic of Croatia, 2015
- Special award of the Ruđer Bošković Institute for outstanding contribution to the scientific excellence and international recognition of the institute, 2015
- European Biophysical Societies Association EBSA Young Investigators' Medal and Prize, 2015
- 5000th ERC grantee, 2015
- MRAK award for creativity in science awarded for the collaboration with the Ruđer Bošković Institute
- Order of Danica Hrvatska for science, outstanding contributions to science and its promotion in the Republic of Croatia and the world, 2014
- European Life Science Award in the category Investigator of the Year, 2014
- Chosen by the journal Cell as one of 40 scientists from around the world and working in diverse biological fields, "40 under 40", 2014
- 2001-2002: NATO stipend, Danish Natural Science Research Council
Classes
Affiliate Full Professor, Faculty of science, University of Zagreb
Affiliate Full Professor, Faculty of science, University of Split
- PhD Program in Biophysics at the University of Split, Croatia (2008-2020)
- Molecular Cytogenetics at the Faculty of Science, University of Zagreb, Croatia, guest lecturer
- Biophysics of the Cell at the Faculty of Science, University of Zagreb, Croatia, guest lecturer
- Biophysics of the Cell Practical at the Faculty of Science, University of Zagreb, Croatia
- Function and regulation of cytoskeleton at the Faculty of Science, University of Zagreb, Croatia, lecturer in charge
Featured Publications
- Matković J., S. Ghosh, M. Ćosić, S. Eibes, M. Barišić, N. Pavin, I.M. Tolić. (2022) Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly. Nat Commun, 13:7307 Download PDF
- Štimac, V., I. Koprivec, M. Manenica, J. Simunić, I.M. Tolić. (2022) Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers. eLife, 11:e83287 Download PDF
- Risteski, P., M. Jagrić, D. Božan, A. Bosilj, N. Pavin, I.M. Tolić. (2022) Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment. Cell Rep. 40 (5): 111169 Download PDF
- Klaasen, S.J., M.Y. Truong, R.H. van Jaarsveld, I. Koprivec, V. Štimac, S. de Vries, P. Risteski, S. Kodba, K. Vukušić, K. de Luca, JF. Marques, E.M. Gerrits, B. Bakker, F. Foijer, J. Kind, I.M. Tolić, S. Lens, G.J.P.L Kops. (2022) Nuclear chromosome locations dictate segregation error frequencies. Nature, 607: 604–609 Download PDF
- Trupinić, M., B. Kokanović, I. Ponjavić, I. Barišić, S. Šegvić, A. Ivec, I.M. Tolić. (2022) The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Curr Biol, 32 (11): 2480-2493 Download PDF
- Vukušić, K., I. Ponjavić, R. Buđa, P. Risteski, I.M. Tolić. (2021) Microtubule sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Dev Cell, 56(13): 1253-1267.e10 Download PDF
- Jagrić, M., P. Risteski., J. Martinčić, A. Milas, I.M. Tolić. (2021) Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. eLife, 10: e61170 Download PDF
- Novak, M., B. Polak, J. Simunić, Z. Boban, B. Kuzmić, A.W. Thomae, I.M. Tolić, N. Pavin. (2018) The mitotic spindle is chiral due to torques within microtubule bundles. Nat Commun, 9(1): 3571. Download PDF
- Vukušić, K., R. Buđa, A. Bosilj, A. Milas, N. Pavin, I.M. Tolić. (2017) Microtubule sliding within the bridging fiber pushes kinetochore fibers apart to segregate chromosomes. Dev Cell, 43(1): 11. Download PDF
- Polak, B., P. Risteski, S. Lesjak, I.M. Tolić. (2017) PRC1-labeled microtubule bundles and kinetochore pairs show one-to-one association in metaphase. EMBO Rep, 18(2): 217. Download PDF
- Kajtez, J., A. Solomatina, M. Novak, B. Polak, K. Vukušić, J. Rüdiger, G. Cojoc, A. Milas, I. Šumanovac Šestak, P. Risteski, F. Tavano, A.H. Klemm, E. Roscioli, J. Welburn, D. Cimini, M. Glunčić, N. Pavin, I.M. Tolić. (2016) Overlap microtubules link sister k-fibers and balance the forces on bioriented kinetochores. Nat. Commun. 7: 10298. Download PDF
- Total number of papers in peer-reviewed journals: 99
- Total number of citations: 7822 (from Google Scholar)
- h-index: 38 (from Google Scholar)
Publications
Books and book chapters
Book chapters
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Ponjavić, I., Vukušić, K. & Tolić, I. (2021) Expansion microscopy of the mitotic spindle. U: Guichard, P. & Hamel, V. (ur.) Methods in Cell Biology. Cambridge, MA, United States, Elsevier, str. 247-274 doi:10.1016/bs.mcb.2020.04.014.
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Milas, A., Jagrić, M., Martinčić, J. & Tolić, I. (2018) Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. U: Maiato, H. & Schuh, M. (ur.) Methods in Cell Biology. USA, Elsevier, str. 191-215 doi:10.1016/bs.mcb.2018.03.024.
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Buđa, R., Vukušić, K. & Tolić, I. (2017) Dissection and characterization of microtubule bundles in the mitotic spindle using femtosecond laser ablation. U: Lecuti, T. (ur.) Cell Polarity and Morphogenesis / Methods in Cell Biology. -, Elsevier, str. 81-101 doi:10.1016/bs.mcb.2016.11.007.
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Ananthanarayanan, V. & Tolić, I. (2015) Single-molecule imaging of cytoplasmic dynein in vivo. U: Paluch, E. (ur.) Biophysical Methods in Cell Biology / Methods in Cell Biology. -, Elsevier, str. 1-12 doi:10.1016/bs.mcb.2014.10.001.
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Maghelli, N. & Tolić-Norrelykke, I. (2011) Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system. U: Straube, A. (ur.) Microtubule Dynamics: Methods and Protocols / Methods in Molecular Biology. -, Springer Nature, str. 261-271 doi:10.1007/978-1-61779-252-6_19.
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Maghelli, N. & Tolić-Norrelykke, I. (2010) Optical trapping and laser ablation of microtubules in fission yeast. U: Cassimeris, L. & Tran, P. (ur.) Microtubules: in vivo / Methods in Cell Biology. -, Elsevier, str. 173-183 doi:10.1016/S0091-679X(10)97010-6.
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Journal articles
Scientific and review papers
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Ban, I., Tomašić, L., Trakala, M., Tolić, I. & Pavin, N. (2023) Proliferative advantage of specific aneuploid cells drives evolution of tumor karyotypes. Biophysical Journal, 122 (4), 632-645 doi:10.1016/j.bpj.2023.01.017.
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Štimac, V., Koprivec, I., Manenica, M., Simunić, J. & Tolić, I. (2022) Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers. eLife, 11, e83287, 26 doi:10.7554/elife.83287.
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Matković, J., Ghosh, S., Ćosić, M., Eibes, S., Barišić, M., Pavin, N. & Tolić, I. (2022) Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly. Nature Communications, 13 (1), 7307, 18 doi:10.1038/s41467-022-34957-4.
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Klaasen, S., Truong, M., van Jaarsveld, R., Koprivec, I., Štimac, V., de Vries, S., Risteski, P., Kodba, S., Vukušić, K., de Luca, K., Marques, J., Gerrits, E., Bakker, B., Foijer, F., Kind, J., Tolić, I., Lens, S. & Kops, G. (2022) Nuclear chromosome locations dictate segregation error frequencies. Nature, 607 (7919), 604-609 doi:10.1038/s41586-022-04938-0.
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Trupinić, M., Kokanović, B., Ponjavić, I., Barišić, I., Šegvić, S., Ivec, A. & Tolić, I. (2022) The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology, 32 (11), 2480-2493e6 doi:10.1016/j.cub.2022.04.035.
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Risteski, P., Božan, D., Jagrić, M., Bosilj, A., Pavin, N. & Tolić, I. (2022) Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment. Cell Reports, 40 (5), 111169, 14 doi:10.1016/j.celrep.2022.111169.
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Vukušić, K. & Tolić, I. (2022) Polar Chromosomes - Challenges of a Risky Path. Cells, 11 (9), 1531, 27 doi:10.3390/cells11091531.
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Velle, K., Kennard, A., Trupinić, M., Ivec, A., Swafford, A., Nolton, E., Rice, L., Tolić, I., Fritz-Laylin, L. & Wadsworth, P. (2022) Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current biology, 32, 1-15 doi:10.1016/j.cub.2022.01.034.
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Ivec, A., Trupinić, M., Tolić, I. & Pavin, N. (2021) Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles. Biophysical journal, 120 (17), 3641-3648 doi:10.1016/j.bpj.2021.07.024.
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Vukušić, K. & Tolić, I. (2021) Anaphase B: Long-standing models meet new concepts. Seminars in Cell & Developmental Biology, 117, 127-139 doi:10.1016/j.semcdb.2021.03.023.
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Risteski, P., Jagrić, M., Pavin, N. & Tolić, I. (2021) Biomechanics of chromosome alignment at the spindle midplane. Current biology, 31 (10), R574-R585 doi:10.1016/j.cub.2021.03.082.
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Vukušić, K., Ponjavić, I., Buđa, R., Risteski, P. & Tolić, I. (2021) Microtubule-sliding modules based on kinesins EG5 and PRC1-dependent KIF4A drive human spindle elongation. Developmental cell, 56 (9), 1253-1267e10 doi:10.1016/j.devcel.2021.04.005.
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Tolić, I. & Pavin, N. (2021) Mitotic spindle: lessons from theoretical modeling. Molecular biology of the cell, 32 (3), 218-222 doi:10.1091/mbc.e20-05-0335.
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Jagrić, M., Risteski, P., Martinčić, J., Milas, A. & Tolić, I. (2021) Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. eLife, 10, e61170, 79 doi:10.7554/eLife.61170.
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Vukušić, K., Buđa, R. & Tolić, I. (2019) Force-generating mechanisms of anaphase in human cells. Journal of cell science, 132 (18), jcs231985, 11 doi:10.1242/jcs.231985.
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Prelogović, M., Winters, L., Milas, A., Tolić, I. & Pavin, N. (2019) Pivot-and-bond model explains microtubule bundle formation. Physical review. E, 100, 012403, 14 doi:10.1103/PhysRevE.100.012403.
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Winters, L., Ban, I., Prelogović, M., Kalinina, I., Pavin, N. & Tolić, I. (2019) Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly. Bmc biology, 17 (1), 42, 18 doi:10.1186/s12915-019-0656-2.
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Tolić, I., Novak, M. & Pavin, N. (2019) Helical Twist and Rotational Forces in the Mitotic Spindle. Biomolecules, 9 (4), 132, 10 doi:10.3390/biom9040132.
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Novak, M., Polak, B., Simunić, J., Boban, Z., Kuzmić, B., Thomae, A., Tolić, I. & Pavin, N. (2018) The mitotic spindle is chiral due to torques within microtubule bundles. Nature Communications, 9, 3571, 10 doi:10.1038/s41467-018-06005-7.
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Klemm, A., Bosilj, A., Glunčić, M., Pavin, N. & Tolić, I. (2018) Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability. Molecular biology of the cell, 29 (11), 1332-1345 doi:10.1091/mbc.E17-11-0667.
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Tolić, I. (2018) Mitotic spindle: Kinetochore fibers hold on tight to interpolar bundles. European biophysics journal, 47 (3), 191-203 doi:10.1007/s00249-017-1244-4.
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Strelnikova, N., Sauter, N., Guizar- Sicairos, M., Göllner, M., Diaz, A., Delivani, P., Chacon, M., Tolić, I., Zaburdaev, V. & Pfohl, T. (2017) Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast. Scientific Reports, 2017 (7), 13775, 7 doi:10.1038/s41598-017-13175-9.
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Vukušić, K., Buđa, R., Bosilj, A., Milas, A., Pavin, N. & Tolić, I. (2017) Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes. Developmental cell, 43 (1), 11-23e6 doi:10.1016/j.devcel.2017.09.010.
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Polak, B., Risteski, P., Lesjak, S. & Tolić, I. (2017) PRC1‐labeled microtubule bundles and kinetochore pairs show one‐to‐one association in metaphase. EMBO reports, 18, 217-230 doi:10.15252/embr.201642650.
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Chacon, M., Delivani, P. & Tolić, I. (2016) Meiotic Nuclear Oscillations Are Necessary to Avoid Excessive Chromosome Associations. Cell Reports, 17 (6), 1632-1645 doi:10.1016/j.celrep.2016.10.014.
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Cojoc, G., Ana-Maria, F., Krull, A., Anna H., K., Pavin, N., Jülicher, F. & Tolić, I. (2016) Paired arrangement of kinetochores together with microtubule pivoting and dynamics drive kinetochore capture in meiosis I. Scientific Reports, 6, 25736, 11 doi:10.1038/srep25736.
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Pavin, N., Tolić, I. & (2016) Self-Organization and Forces in the Mitotic Spindle. Annual Review of Biophysics, 45, 279-298 doi:10.1146/annurev-biophys-062215-010934.
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Cojoc, G., Roscioli, E., Zhang, L., García-Ulloa, A., Shah, J., Berns, M., Pavin, N., Cimini, D., Tolić, I. & Gregan, J. (2016) Laser microsurgery reveals conserved viscoelastic behavior of the kinetochore. The Journal of cell biology, 212 (7), 767-776 doi:10.1083/jcb.201506011.
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Simunić, J. & Tolić, I. (2016) Mitotic Spindle Assembly : Building the Bridge between Sister K-Fibers. Trends in biochemical sciences, 41 (10), 824-833 doi:10.1016/j.tibs.2016.07.004.
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Kajtez, J., Solomatina, A., Novak, M., Polak, B., Vukušić, K., Rüdiger, J., Cojoc, G., Milas, A., Šumanovac Šestak, I., Risteski, P., Tavano, F., H. Klemm, A., Roscioli, E., Welburn, J., Cimini, D., Glunčić, M., Pavin, N. & Tolić, I. (2016) Overlap microtubules link sister k-fibres and balance the forces on bi-oriented kinetochores. Nature Communications, 7, 10298, 11 doi:10.1038/ncomms10298.
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Lade, S., Coelho, M., Tolić, I. & Gross, T. (2015) Fusion leads to effective segregation of damage during cell division: An analytical treatment. Journal of theoretical biology, 378, 47-55 doi:10.1016/j.jtbi.2015.04.022.
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Lin, Y., Frömberg, D., Huang, W., Delivani, P., Chacón, M., Tolić, I., Jülicher, F. & Zaburdaev, V. (2015) Pulled Polymer Loops as a Model for the Alignment of Meiotic Chromosomes. Physical Review Letters, 115, 208102-1 doi:10.1103/PhysRevLett.115.208102.
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Glunčić, M., Maghelli, N., Krull, A., Krstić, V., Ramunno-Johnson, D., Pavin, N., Tolić, I. & (2015) Kinesin-8 Motors Improve Nuclear Centering by Promoting Microtubule Catastrophe. Physical Review Letters, 114, 078103-1 doi:10.1103/PhysRevLett.114.078103.
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Guarino, E., Cojoc, G., García-Ulloa, A., Tolić, I. & Kearsey, S. (2014) Real-time imaging of DNA damage in yeast cells using ultra-short near-infrared pulsed laser irradiation. PLoS ONE, 9 (11), e113325, 9 doi:10.1371/journal.pone.0113325.
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Klingner, C., Cherian, A., Fels, J., Diesinger, P., Aufschnaiter, R., Maghelli, N., Keil, T., Beck, G., Tolić-Nørrelykke, I., Bathe, M. & Wedlich-Soldner, R. (2014) Isotropic actomyosin dynamics promote organization of the apical cell cortex in epithelial cells. The Journal of cell biology, 207 (1), 107-121 doi:10.1083/jcb.201402037.
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Krull, A., Steinborn, A., Ananthanarayanan, V., Ramunno-Johnson, D., Petersohn, U. & Tolić-Norrelykke, I. (2014) A divide and conquer strategy for the maximum likelihood localization of low intensity objects. Optics express, 22 (1), 210-228 doi:10.1364/OE.22.000210.
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Baumgärtner, S. & Tolić, I. (2014) Astral Microtubule Pivoting Promotes Their Search for Cortical Anchor Sites during Mitosis in Budding Yeast. PLoS one, 9, e93781, 8 doi:10.1371/journal.pone.0093781.
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Coelho, M., Lade, S., Alberti, S., Gross, T. & Tolić, I. (2014) Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation. PLoS biology, 12 (6), e1001886, 11 doi:10.1371/journal.pbio.1001886.
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Pavin, N. & Tolić‑Nørrelykke, I. (2014) Swinging a sword : how microtubules search for their targets. Systems and synthetic biology, 8 (3), 179-186 doi:10.1007/s11693-014-9134-x.
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Coelho, M., Maghelli, N. & Tolić- Norrelykke, I. (2013) Single-molecule imaging in vivo: The dancing building blocks of the cell. Integrative Biology, 5 (5), 748-758 doi:10.1039/c3ib40018b.
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Coelho, M., Dereli, A., Haese, A., Kühn, S., Malinovska, L., Desantis, M., Shorter, J., Alberti, S., Gross, T. & Tolić-Norrelykke, I. (2013) Fission Yeast Does Not Age under Favorable Conditions, but Does So after Stress. Current biology, 23 (19), 1844-1852 doi:10.1016/j.cub.2013.07.084.
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Pavin, N. & Tolić‑Nørrelykke, I. (2013) Dynein, microtubule and cargo: a ménage à trois. Biochemical Society transactions, 41 (6), 1731-1735 doi:10.1042/BST20130235.
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Ananthanarayanan, V., Schattat, M., Vogel, S., Krull, A., Pavin, N. & Tolić-Nørrelykke, I. (2013) Dynein motion switches from diffusive to directed upon cortical anchoring. Cell, 153 (7), 1526-1536 doi:10.1016/j.cell.2013.05.020.
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Kalinina, I., Nandi, A., Delivani, P., Chacón, M., Klemm, A., Ramunno-Johnson, D., Krull, A., Lindner, B., Pavin, N. & Tolić-Nørrelykke, I. (2013) Pivoting of microtubules around the spindle pole accelerates kinetochore capture. Nature cell biology, 15, 82-87 doi:10.1038/ncb2640.
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Goudarzi, M., Banisch, T., Mobin, M., Maghelli, N., Tarbashevich, K., Strate, I., van den Berg, J., Blaser, H., Bandemer, S., Paluch, E., Bakkers, J., Tolić-Norrelykke, I. & Raz, E. (2012) Identification and Regulation of a Molecular Module for Bleb-Based Cell Motility. Developmental cell, 23 (1), 210-218 doi:10.1016/j.devcel.2012.05.007.
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Gregan, J., Polakova, S., Zhang, L., Tolić-Norrelykke, I. & Cimini, D. (2011) Merotelic kinetochore attachment: causes and effects. Trends in cell biology, 21 (6), 374-381 doi:10.1016/j.tcb.2011.01.003.
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Tolić-Norrelykke, I. (2010) Force and length regulation in the microtubule cytoskeleton: lessons from fission yeast. Current opinion in cell biology, 22 (1), 21-28 doi:10.1016/j.ceb.2009.12.011.
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Rumpf, C., Cipak, L., Schleiffer, A., Pidoux, A., Mechtler, K., Tolić-Norrelykke, I. & Gregan, J. (2010) Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4. Cell cycle, 9 (19), 3997-4004 doi:10.4161/cc.9.19.13233.
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Stiess, M., Maghelli, N., Kapitein, L., Gomis-Rüth, S., Wilsch-Bräuninger, M., Hoogenraad, C., Tolić- Nørrelykke, I. & Bradke, F. (2010) Axon extension occurs independently of centrosomal microtubule nucleation. Science, 327 (5966), 704-707 doi:10.1126/science.1182179.
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Raabe, I., Vogel, S., Peychl, J. & Tolić- Norrelykke, I. (2009) Intracellular nanosurgery and cell enucleation using a picosecond laser. Journal of microscopy, 234 (1), 1-8 doi:10.1111/j.1365-2818.2009.03142.x.
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Baumgärtner, S. & Tolić-Norrelykke, I. (2009) Growth pattern of single fission yeast cells is bilinear and depends on temperature and DNA synthesis. Biophysical journal, 96 (10), 4336-4347 doi:10.1016/j.bpj.2009.02.051.
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Vogel, S., Pavin, N., Maghelli, N., Julicher, F. & Tolić-Norrelykke, I. (2009) Microtubules and motor proteins : Mechanically regulated self-organization in vivo. European physical journal-special topics, 178, 57-69 doi:10.1140/epjst/e2010-01182-6.
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Vogel, S., Pavin, N., Maghelli, N., Julicher, F. & Tolić-Norrelykke, I. (2009) Self-organization of dynein motors generates meiotic nuclear oscillations. PLoS biology, 7 (4), 918-928 doi:10.1371/journal.pbio.1000087.
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Maghelli, N. & Tolić-Norrelykke, I. (2008) Versatile laser-based cell manipulator. Journal of Biophotonics, 1 (4), 299-309 doi:10.1002/jbio.200810026.
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Krüger, N. & Tolić-Norrelykke, I. (2008) Association of mitochondria with spindle poles facilitates spindle alignment. Current biology, 18 (15), R646-R647 doi:10.1016/j.cub.2008.06.069.
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Tolić-Norrelykke, I. (2008) Push-me-pull-you: how microtubules organize the cell interior. European biophysics journal, 37 (7), 1271-1278 doi:10.1007/s00249-008-0321-0.
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Howard, J. & Tolić-Nørrelykke, I. (2007) Bundling, sliding, and pulling microtubules in cells and in silico. HFSP journal, 1 (1), 11-14 doi:10.2976/1.2740563.
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Vogel, S., Raabe, I., Dereli, A., Maghelli, N. & Tolić-Nørrelykke, I. (2007) Interphase microtubules determine the initial alignment of the mitotic spindle. Current biology, 17 (5), 438-444 doi:10.1016/j.cub.2007.01.047.
doi Munteanu, E., Olsen, A., Tolić-Nørrelykke, I., Flyvbjerg, H., Oddershede, L. & Berg-Sørensen, K. (2006) Quantitative studies of subdiffusion in living cells and actin networks. Biophysics reviews and letters, 1, 411-421.
Sacconi, L., Tolić-Nørrelykke, I., D’Amico, M., Vanzi, F., Olivotto, M., Antolini, R. & Pavone, F. (2006) Cell imaging and manipulation by nonlinear optical microscopy. Cell biochemistry and biophysics, 45 (3), 289-302.
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Galimberti, M., Tolić-Nørrelykke, I., Favillini, R., Mercatelli, R., Annunziato, F., Cosmi, L., Liotta, F., Santarlasci, V., Maggi, E. & Pavone, F. (2006) Hypergravity speeds up the development of T-lymphocyte motility. European biophysics journal, 35 (5), 393-400 doi:10.1007/s00249-006-0046-x.
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Hansen, P., Tolić-Nørrelykke, I., Flyvbjerg, H. & Berg-Sørensen, K. (2006) tweezercalib 2.0 : Faster version of MatLab package for precise calibration of optical tweezers. Computer physics communications, 174 (6), 518-520 doi:10.1016/j.cpc.2005.11.007.
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Sacconi, L., Tolić-Nørrelykke, I., Antolini, R. & Pavone, F. (2005) Combined intracellular three-dimensional imaging and selective nanosurgery by a nonlinear microscope. Journal of biomedical optics, 10 (1), 014002-1 doi:10.1117/1.1854675.
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Sacconi, L., Tolić-Nørrelykke, I., Stringari, C., Antolini, R. & Pavone, F. (2005) Optical micromanipulations inside yeast cells. Applied optics, 44 (11), 2001-2007 doi:10.1364/AO.44.002001.
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Tolić-Nørrelykke, I., Sacconi, L., Stringari, C., Raabe, I. & Pavone, F. (2005) Nuclear and division-plane positioning revealed by optical micromanipulation. Current biology, 15 (13), 1212-1216 doi:10.1016/j.cub.2005.05.052.
doi Tolić-Norrelykke, I. & Wang, N. (2005) Traction in smooth muscle cells is associated with cell morphology. Journal of biomechanics, 38 (7), 1405-1412.
Tolić-Norrelykke, I., Munteanu, E., Thon, G., Oddershede, L. & Berg-Sorensen, K. (2004) Anomalous diffusion in living yeast cells. Physical review letters, 93 (7).
Tolić-Norrelykke, I., Sacconi, L., Thon, G. & Pavone, F. (2004) Positioning and elongation of the fission yeast spindle by microtubule-based pushing. Current biology, 14, 1181-1186.
Tolić-Norrelykke, I., Berg-Sorensen, K. & Flyvbjerg, H. (2004) MatLab program for precision calibration of optical tweezers. Computer physics communications, 159, 225-240.
Tolić-Norrelykke, I. & Wang, N. (2003) Traction, trypsin, and tensegrity. Internet Electronic Journal of Molecular Design, 2.
Stamenović, D., Mijailovich, Srboljub, M., Tolić-Norrelykke, Iva, Marija & Wang, N. (2003) Experimental tests of the cellular tensegrity hypothesis. Biorheology (Oxford), 40 (1-3 , special issue), 221-225.
Wang, N., Tolić-Norrelykke, I., Chen, J., Mijailovich, Srboljub, M., Butler, J., Fredberg, J. & Stamenović, D. (2002) Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells. American Journal of Physiology - Cell Physiology, 282 (3), C606-C616. (https://www.bib.irb.hr/84216).
Stamenović, D., Mijailovich, Srboljub, M., Tolić-Norrelykke, I., Chen, J. & Wang, N. (2002) Cell prestress. II. Contribution of microtubules. American Journal of Physiology - Cell Physiology, 282 (3), C617-C624. (https://www.bib.irb.hr/84034).
Butler, James, P., Tolić-Norrelykke, Iva, Marija, Fabry, B. & Fredberg, Jeffrey, J. (2002) Traction fields, moments, and strain energy that cells exert on their surroundings. American Journal of Physiology - Cell Physiology, 282 (3), C595-C605. (https://www.bib.irb.hr/82590).
Tolić-Norrelykke, I., Butler, J., Chen, J. & Wang, N. (2002) Spatial and temporal traction response in human airway smooth muscle cells. American Journal of Physiology - Cell Physiology, 283 (4), C1254-C1266. (https://www.bib.irb.hr/81780).
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Wang, N., Naruse, K., Stamenović, D., Fredberg, J., Mijailovich, S., Tolić-Norrelykke, I., Polte, T., Mannix, R. & Ingber, D. (2001) Mechanical behavior in living cells consistent with the tensegrity model. Proceedings of the National Academy of Sciences of the United States of America, 98 (14), 7765-7770 doi:10.1073/pnas.141199598.
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Tolić, I., Mosekilde, E. & Sturis, J. (2000) Modeling the insulin-glucose feedback system: The significance of pulsatile insulin secretion. Journal of Theoretical Biology, 207 (3), 361-375 doi:10.1006/jtbi.2000.2180.
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Nikolić, S., Trinajstić, N. & Tolić, I. (2000) Complexity of molecules. Journal of chemical information and computer sciences, 40 (4), 920-926 doi:10.1021/ci9901183.
doi Nikolić, S., Tolić, I. & Trinajstić, N. (1999) On the Complexity of Molecular Graphs. Communications in mathematical and computer chemistry : Match, 40, 187-201.
Tolić, I. & Trinajstić, N. (1998) Origin of Life: Earth or Outer Space?. Periodicum biologorum, 100, 405-410.
Conference proceedings papers
Scientific conference proceedings papers
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Tišma, S., Kalambura, S., Biondić, R., Kovač, S., Meaški, H., Dogančić, D., Nađ, L., Farkaš, A. & Tolić, I. (2023) COVID-19 impact on the quality of internship program in environmental engineering studies in Croatia – four year analysis. U: Gómez Chova, L., González Martínez, C. & Lees, J. (ur.)INTED2023 Proceedings 17th International Technology, Education and Development Conference Valencia, Spain. 6-8 March, 2023. doi:10.21125/inted.2023.
doilibrary.iated.orglibrary.iated.org
Membership in professional associations / societies
- European Molecular Biology Organization (EMBO)
- Croatian Biophysical Society
- Croatian Society of Biochemistry and Molecular Biology
- Croatian Microscopy Society
Scientific career:
- since 2019: Senior Research Group Leader with tenure and Full Professor, Ruđer Bošković Institute, Zagreb, Croatia
- 2014: Senior Research Group Leader and Full Professor, Ruđer Bošković Institute, Zagreb, Croatia
- 2010 – 2014: Senior Research Group Leader (W2, equivalent to Associate Professor), Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- 2004 – 2009: Research Group Leader (W1, equivalent to Assistant Professor), Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany
- 2003 – 2004: Post-doctoral research with Prof. Francesco Pavone, LENS - European Laboratory for Non-Linear Spectroscopy, Florence, Italy
- 2001 – 2002: Post-doctoral research with Prof. Kirstine Berg-Sørensen, Niels Bohr Institute, Copenhagen, Denmark
Recent discoveries:
- Jagrić et al. discovered that without the molecular ruler proteins, the bridging fibers were also too long. This increased the overlap between the microtubules in the center of the spindle, causing the chromosomes to migrate away from the center. This suggests that the alignment of chromosomes in the middle of the spindle depends on the bridging microtubules, which need to be of a certain length to effectively move and keep the chromosomes at the center. Thus, forces that move the chromosomes are generated both at the tips of the microtubules and along the wall of microtubules (Jagrić, M., P. Risteski., J. Martinčić, et al., eLife 2021).
- By combining super-resolution microscopy and theory, we have shown that the spindle is chiral. Microtubule bundles twist around the spindle axis following a left-handed helical path, which is regulated by kinesin-5. Thus, in addition to forces, torques exist in the spindle and determine its chiral architecture (Novak, Polak, Simunić, Boban, Kuzmić et al., Nat Commun 2018).
- By combining laser ablation, photoactivation, and theory, we have shown that sliding apart of microtubules within the bridging fiber pushes the attached kinetochore fibers and their kinetochores apart, being able to segregate chromosomes independently of the spindle pole (Vukušić, Buđa et al., Dev Cell 2017).
- We have discovered that non-kinetochore microtubules, which we term bridging fibers, balance the tension between sister kinetochores by bridging sister k-fibers (Kajtez, Solomatina, Novak et al., Nat Commun 2016). This study challenges the paradigm that the tension on kinetochores is generated by molecular events occurring only at the ends of the k-fibers.
- By establishing single-molecule observation and quantification of the motor protein dynein in living cells, we have discovered a new mechanism for how dynein targets cortical anchor sites, by diffusing along the microtubule (Ananthanarayanan et al., Cell 2013).
- We have discovered a new mechanism of kinetochore capture during mitosis, based on the pivoting motion of microtubules around the spindle pole (Kalinina et al., Nat. Cell Biol. 2013). This concept challenges the current ‘search-and-capture’ paradigm introduced in the 1980s, based on direct growth of microtubules towards kinetochores.
- We have demonstrated that fission yeast does not undergo replicative aging, which challenges the dogma that all organisms, including microorganisms, are susceptible to aging (Coelho et al., Curr. Biol. 2013; Coelho et al., PLOS Biol. 2014).
Mentoring:
- 11 PhD, 4 Master and 18 Diploma theses completed.
- PhD students have published first-author papers in top journals (Cell, Nature Cell Biology, Developmental Cell, PLoS Biology, Current Biology, EMBO Reports, eLife). Current positions of the former PhD students are the following: Vaishnavi Ananthanarayanan is a Research Group Leader at University of New South Wales; Sven Vogel is a Team Leader at the Max Planck Institute of Biochemistry; Miguel Coelho is a Biotech Equity Research at Truist Securities; Iana Kalinina is a postdoc at the University of Exeter; Davide Accardi is the leader of the Bioimaging Platform at Champalimaud Foundation in Lisbon; Manuel Neetz is Head of IP Diagnostic Imaging at Siemens Healthineers; Kruno Vukušić is a postdoc at the Ruđer Bošković Institute in Zagreb; Bruno Polak is a postdoc at University of Zagreb; Renata Buđa is a biochemist at Fidelta in Zagreb.
Invited talks:
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More than 130 invited talks at conferences and workshops including:
ÖGMBT 13th Annual Meeting, Vienna, Austria, plenary talk, 2021 (virtual)
Physics Meets Biology, Oxford, United Kingdom, keynote talk, 2021 (virtual)
Virtual Biology Colloquium. National University of Singapore, Singapore, 2021 (virtual)
The Biophysical Society 65nd Annual Meeting, 2021 (virtual)
ASCB│EMBO 2019 Meeting, Washington DC, USA, symposia talk 2019.
Gordon Research Conference: Stochastic Physics in Biology, Fluctuations, Landscapes and Statistical Mechanics of Biological Heterogeneity, Ventura, CA, USA, 2019
Biophysical Society 62nd Annual Meeting, San Francisco, CA, USA, 2018
Gordon Research Conference (GRC) on Motile and Contractile Systems, New London, NH, USA, 2017
EMBO Conference on Meiosis, Oxford, UK, 2015
12th Multinational Congress on Microscopy, Eger, Hungary, 2015 (plenary talk)
10th European Biophysics Congress EBSA, Dresden, Germany, 2015 (plenary talk)
The American Society for Cell Biology (ASCB) Annual Meeting, Philadelphia, PA, USA, 2014
Biophysical Society 56th Annual Meeting, San Diego, CA, USA, 2012
Gordon Research Conference (GRC) on Plant and Microbial Cytoskeleton, Andover, NH, USA, 2012 -
More than 40 invited seminars including:
Instituto Gulbenkian de Ciência, virtually , Portugal, 2021
Max Planck Institute for Biophysical Chemistry, Göttingen, Germany, 2020.
Humboldt-Universität zu Berlin, Berlin, Germany, 2019
Max Planck Institute (MPI) for Developmental Biology, Tübingen, Germany, 2018
University of Geneva, Switzerland, 2018
Institut Curie, Paris, France, 2018
ETH Zürich, Switzerland, 2015
Engineering and Physical Biology Symposium, Harvard, Cambridge, MA, USA, 2012
California Institute of Technology, Pasadena, CA, USA, 2010
Organization of scientific meetings:
- Co-organizer of the conference Mitotic spindle: From living and synthetic systems to theory, Split, Croatia, 2019 and 2021
- Member of the International Scientific Committee of the 45th FEBS Congress Molecules of Life: Towards New Horizons, Ljubljana, Slovenia, 2021
- Member of the Scientific Committee of the Students and Postdocs Meiosis Workshop, 2021
- Member of the Program Committee for the Optical Manipulation and its Applications Topical Meeting, OSA Congress on Optics in the Life Sciences, Tucson, AZ, USA, 2019 and 2021 (virtual)
- Co-chair at the 2nd ASCB│EMBO Meeting, San Diego, CA, USA, 2018
- Co-chair at the 62nd Annual Meeting of the Biophysical Society, San Francisco, CA, USA, 2018
- Organizer of the EMBO Conference on Meiosis, Hvar, Croatia, 2017
- Co-organizer of ELMI - 17th European Light Microscopy Initiative meeting, Dubrovnik, Croatia, 2017
- Member of the Program Committee for the 9th International Fission Yeast Meeting, Banff, Alberta, Canada, 2017
- Member of the International Advisory Committee for the conference From Solid State to Biophysics VIII, Cavtat, Croatia, 2016
- Member of the Organizing Committee for the EMBO Conference on Meiosis, Oxford, UK, 2015
- Chairperson of the symposium Molecular Motors at the 10th European Biophysics Congress, EBSA 2015, Dresden, Germany, 2015
- Member of the Program Committee for the EMBO Conference Pombe 2013, 7th International Fission Yeast Meeting, London, UK, 2013
- Member of the Program Committee for the 11th and 12th Greta Pifat Mrzljak International School of Biophysics, Primošten, Croatia, 2012 and 2014
Ad hoc reviewer:
- Journals: Nature, Science, Nature Cell Biology, eLife, Proceedings of the National Academy of Sciences, Current Biology, Molecular Biology of the Cell, EMBO Journal, Optics Express, Physical Biology, Journal of Microscopy, Biochemical and Biophysical Research Communications
- Funding agencies: ERC Starting i Consolidator Grants, German Research Foundation (DFG), Human Frontier Science Program, Swiss National Science Foundation, Academy of Sciences of the Czech Republic, The Leverhulme Trust.
Editorial activities:
- Member of the Advisory Editorial Board of Life Science Alliance
- Member of the Scientific Advisory Board and the Editorial Board of Science Matters
Work in panels and international commissions
- Member of the ERC Starting Grant Panel 2020
- Member of the the National Fund for Scientific Research (FNRS) Quinquennial Prize Panel, Belgium, 2020.
- Member of the German Science Foundation (DFG) Panel for Collaborative Research Centers/ Transregio, 2017.
Public outreach and media coverage:
- Guest in Croatian TV shows Hrvatska Za 5, Treći element, Društvena mreža, and Među nama; interviews for TV shows Labirint, Znanstveni krugovi, and Dobro jutro, Hrvatska; numerous short interviews for TV news on Croatian Radiotelevision 1, Nova TV and RTL
- Interviews on the radio: BBC World Service, Croatian Radio International Channel "The Voice of Croatia", Croatian Radio 3, Radio Sljeme, Radio Yammat
- Interviews in newspapers and magazines: NASA's Astrobiology Journal (USA); Bild (Germany); Science et Vie (France); Croatia Airlines Inflight Magazine, Jutarnji list, Večernji list, Gloria, Novi List, Slobodna Dalmacija, 24 sata, Tehnopolis, Supermila (Croatia)
- Motivational talks for pupils from more than seven middle and high schools
- 5 popular science lectures
- Hosted the President of the European Research Council (ERC), Prof Jean-Pierre Bourguignon, in the lab in March 2018
- Hosted the President of the Republic of Croatia, Kolinda Grabar-Kitarović, in the lab in March 2017
- Speech to the European Parliament in Brussels, June 2015
- Chosen as one of the "Stars of 2015" by Gloria
- Chosen as one of "The Most Stylish Scientists" by Future-ish, 2013-2015