-       UniversityofZagreb, Faculty of Science; B. Sc., 1994, Biology-Ecology

-       UniversityofZagreb, Faculty of Science; Mr. Sc., 1998, Biology-Toxicology

-       UniversityofZagreb, Faculty of Science; PhD, 1999, Biology-Ecotoxicology

Ongoing:

-        Ecotoxicological characterization of biologically active substances and complex marine environment samples – Project within the Centre for Excellence for Science and Technology – Integration of Mediterranean Region (ZCI-STIM;  https://stim.unist.hr/hr/) (2017-2021) – project funded by the European Union through the European Regional Development Fund – the Operational Programme Competitiveness and Cohesion 2014-2020 (KK.01.1.1.01); principal investigator.

-      Understanding the (eco)toxicological role of selected SLC and MATE transport proteins in zebrafish (Danio rerio) using functional genomics tools, (2020-2023) – Croatian Science Foundation research project – principal investigator;

Educational Activities/Lecturing

-        M. Erk, T. Smital – Course Biological effects of metals and organic pollutants on marine organisms; Postgraduate study, Oceanology, Faculty of Science, University of Zagreb;

-        T. Smital – Course Ecotoxicology; Postgraduate study "Protection of Nature and the Environment", University of Osijek;

-        G. Klobučar, M. Pavlica, V. Garaj-Vrhovac, T. Smital – Course Biomarkers in Biomonitoring of Environmental Contamination; Postgraduate study in Biology, Faculty of Science, University of Zagreb.

Science Citation Index Publications

1. Smital T, Pivčević B. and Kurelec B. (1996) Reversal of multidrug resistance by extract from the marine alga Caulerpa taxifolia. Period. Biol. 98: 165-171.
2. Smital T. and Kurelec B. (1997) Inhibitors of the multixenobiotic resistance mechanism in natural waters: In vivo demonstration of their effects. Environ. Toxicol. Chem. 16: 2164-2170.
3. Kurelec B., Smital T., Britvić S., Pivčević B., Krča S., Jelaska D., Balen S., Sauerborn R. and Mustajbegović S. (1997) Multixenobiotic defence mechanism in aquatic organisms. Period. Biol. 99: 319-328.
4. Smital. T. and Kurelec B. (1998) The chemosensitizers of multixenobiotic resistance mechanism in aquatic invertebrates; A new class of pollutants. Mutat. Res.-Fund. Mol. M. 399: 45-53.
5. Kurelec B., Britvić S. and Smital T. (1998) Fragility of multixenobiotic resistance in aquatic organisms enhances the complexity of risk assessment. Mar. Environ. Res. 46: 415-419.
6. Smital T. and Kurelec B. (1998) The activity of multixenobiotic resistance mechanism determined by rhodamine B - efflux method as a biomarker of exposure. Mar. Environ. Res. 46: 443-447.
7. Osmak M., Brozović A., Ambriović-Ristov A., Hadžija M., Pivčević B., and Smital T. (1998) Inhibition of apoptosis is the cause of resistance to doxorubicin in human breast adenocarcinoma cells. Neoplasma 45: 223-230.
8. Kurelec, B., Smital, T., Pivčević, B., Eufemia, N. and Epel, D. (2000) Multixenobiotic resistance, P-glycoprotein, and chemosensitizers. Ecotoxicology 9: 307-327.
9. Smital, T., Sauerborn, R., Pivčević, B., Krča, S. and Kurelec, B. (2000) Interspecies differences in P-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates, Comp. Biochem. Physiol C., 126: 175-186.
10. Ramljak, S., Hackenberger, B.K., Smital, T. and Britvić, S. (2000) Evaluation of the genotoxic and cytochrome P450 monooxygenase-inhibitory potential of dicuran on procariotic and eucaryotic test systems, J.  Environ. Sci. Health B. B35: 751-770.
11. Smital, T. and Sauerborn, R. (2002) Measurement of the activity of multixenobiotic resistance mechanism in the common carp Cyprinus carpio. Mar. Environ. Res. 54: 449-453.
12. Smital, T., Sauerborn, R. and Hackenberger, B.K. (2003) Inducibility of the P-glycoprotein transport activity in the marine mussel Mytilus galloprovincialis and the freshwater mussel Dreissena polymorpha. Aquat. Toxicol. 65: 443-465.
13. Sauerborn, R., Stupin Polancec, D., Zaja, R. and Smital, T. (2004) Identification of the multidrug resistance-associated protein (MRP) related gene in the red mullet (Mullus barbatus). Mar. Environ. Res., 58: 199-204.
14. Smital, T., Luckenbach, T., Sauerborn, R., Hamdoun, A.M., Vega, R.L. and Epel, D. (2004) Emerging contaminants – pesticides, PPCPs, microbial degradation products and natural substances as inhibitors of multixenobiotic defense in aquatic organisms. Mut. Res.-Fund. Mol. M., 552: 101-117.
15. Žaja, R., Klobučar, G.I.V., Sauerborn Klobučar, R., Hackenberger, B., and Smital, T. (2006) Haemolymph as compartment for efficient and non-destructive determination of Pgp mediated MXR activity in bivalves. Comp. Biochem. Physiol. C., 143: 103-112.
16. Žaja, R., Sauerborn Klobučar R. and Smital, T. (2007) Detection and functional characterization of Pgp1 (ABCB1) and MRP3 (ABCC3) efflux transporters in the PLHC-1 fish hepatoma cell line. Aquat. Toxicol., 81: 365-376.
17. Krča, S., Žaja, R., Čalić, V., Terzić, S., Grubešić, M.S., Ahel, M. and Smital, T. (2007) Hepatic biomarker responses to organic contaminants in feral chub (Leuciscus cephalus) – laboratory characterization and field study in the Sava River, Croatia. Environ. Toxicol. Chem., 26: 2620-2633.
18. Žaja, R., Caminada, D., Lončar, J., Fent, K. and Smital, T. (2008) Development and characterization of P-glycoprotein 1 (Pgp1; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line. Toxicol. Appl. Pharmacol., 227: 207-218.
19. Žaja, R., Munić, V., and Smital, T. (2008) Cloning and mRNA expression analysis of an ABCG2 (BCRP) efflux transporter in rainbow trout (Oncorhynchus mykiss) liver and primary hepatocytes. Mar. Environ. Res., 66: 77-79.
20. Källqvist, T., Milačič, R., Smital, T., Thomas, K.V., Vranes, S. and Tollefsen, K-E. (2008) Chronic toxicity of the Sava River (SE Europe) sediments and river water to the algae Pseudokirchneriella subcapitata. Water Res., 42: 2146-2156.
21. Epel, D., Stevenson, C.N., MacManus-Spencer, L.A., Luckenbach, T., Hamdoun, A., and Smital, T. (2008) Efflux transporters: newly appreciated roles in protection against pollutants. Environ. Sci. Tech., 42: 3914-3920.
22. Traven, L., Žaja, R., Lončar, J., Smital, T. and Mićović, V. (2008) CYP1A induction potential and the concentration of priority pollutants in marine sediment samples – In vitro evaluation using the PLHC-1 fish hepatoma cell line. Toxicol. In Vitro, 22: 1648-1656.
23. Caminada, D., Žaja, R., Smital, T. and Fent, K. (2008) Human pharmaceuticals modulate P-gp1 (ABCB1) transport activity in the fish cell line PLHC-1. Aquat. Toxicol., 90: 214–222.
24. Žaja, R., Munić, V., Sauerborn Klobučar, R., Ambriović-Ristov, A., Smital, T. (2008) Cloning and functional characterization of apical efflux transporters (ABCB1, ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquat. Toxicol. 90: 322-332.
25. Bošnjak, I., Heim, W., Smital, T., Epel, D., Coale, K., Franekić-Čolić, J., Hamdoun, A. (2009) Multidrug resistance associated protein transport activity mediates differences in accumulation and toxicity of inorganic and organic mercury in sea urchin embryos. Environ. Sci. Tech. 43: 8374-8380.
26. Lončar, J., Popović, M., Žaja, R., Smital, T. (2010) Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. C. 151: 209-215.
27. Popović, M., Žaja, R., Lončar, J., Smital, T. (2010) A novel ABC transporter: the first insight into zebrafish (Danio rerio) Abch1. Mar. Environ. Res. 69: S11-S13.
28. Popović, M., Žaja, R., Smital, T. (2010) Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): phylogenetic analysis and tissue distribution. Comp. Biochem. Physiol. A 155: 327-335.  
29. Sauerborn Klobučar, R., Zaja, R., Franjević, D., Brozović, A., Smital, T. (2010) Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish. Arh. Hig. Rada Toksikol. 61: 175-181.
30. Bošnjak, I., Šegvić, T., Smital, T., Franekić, J., Mladineo, I. (2011) Sea Urchin Embryotoxicity Test for Environmental Contaminants—Potential Role of the MRP Proteins. Water Air Soil Poll. 217: 627-636.
31. Fischer, S., Loncar, J., Zaja, R., Schnelle, S., Schirmer, K., Smital, T., Luckenbach, T. (2011) Constitutive mRNAexpression and protein activity levels of nine ABC efflux transporters in seven permanent cell lines derived from different tissues of rainbow trout (Oncorhynchus mykiss). Aquat. Toxicol. 101: 438-446.
32. Smital, T., Terzic, S., Zaja, R., Senta, I., Pivcevic, B., Popovic, M., Mikac, I., Tollefsen, K.E., Thomas, K.V., Ahel, M. (2011) Assessment of toxicological profiles of the municipal wastewater effluents using chemical analyses and bioassays. Ecotoxicol. Environ. Saf. 74: 844-851.
33. Zaja, R., Loncar, J., Popovic, M., Smital, T. (2011) First characterization of fish P-glycoprotein (abcb1) substrate specificity using determinations of its ATPase activity and calcein-AM assay with PLHC-1/dox cell line. Aquat. Toxicol. 103: 53-62.
34. Reifferscheid, G., Maes, H., Allner, B., Badurova, J., Belkin, S., Blum, K., Brauer, F., Bressling, J., Domeneghetti, S., Elad, T., Flückiger-Isler, S., Grummt, H.J., Guertler, R., Hecht, A., Heringa, M., Hollert, H., Huber, S., Kramer, M., Magdeburg, A., Ratte, T., Sauerborn-Klobucar, R., Sokolowski, A., Soldan, P., Smital, T., Stalter, D., Venier, P., Ziemann, C., Zipperle, J., Buchinger, S. (2012) International round-robin study on the Ames fluctuation test. Environ. Mol. Mutag. 53: 185-197.
35. Della Torre, C., Zaja, R., Loncar, J., Smital, T., Focardi, S., Corsi, I. (2012) Interaction of ABC transport proteins with toxic metals at the level of gene and transport activity in the PLHC-1 fish cell line. Chem. Biol. Interac. 198: 9-17.
36. Smital, T., Terzić, S., Lončar, J., Senta, I., Zaja, R., Popović, M., Mikac, I., Tollefsen, K-E., Thomas, K.V., Ahel, M. (2013) Prioritisation of organic contaminants in a river basin using chemical analyses and bioassays. Environ. Sci. Poll. Res. 20: 1384-1395.
37. Zaja, R., Terzić, S., Senta, I., Lončar, J., Popović, M., Ahel, M., Smital, T. (2013) Identification of P-glycoprotein (P-gp, Abcb1) inhibitors in contaminated freshwater sediments. Environ. Sci. Tech. 47: 4813-4821.
38. Traven, L., Mićović, V., Vukić Lušić, D., Smital, T. (2013) The responses of the hepatosomatic index (HSI), 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione-S-transferase (GST) activity in sea bass (Dicentrarchus labrax, Linnaeus 1758) caged at a polluted site: implications for their use in environmental risk assessment. Environ Monit Assess. 185: 9009-9018.
39. Bošnjak, I., Zaja, R., Klobučar, R.S., Sver, L., Franekić, J., Smital, T. (2013) Identification of ABC Transporter Genes in Gonad Tissue of Two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L. Bull. Environ. Contam. Toxicol. 91: 415-419. 
40. Popovic, M., Zaja, R., Fent, K., Smital, T. (2013) Molecular characterization of zebrafish Oatp1d1 (Slco1d1), a novel Organic anion transporting polypeptide. J. Biol. Chem. 288: 33894–33911.
41. Ferreira, M., Santos, P., Rey-Salgueiro, L., Zaja, R., Reis-Henriques, M.A., Smital, T. (2014) The first demonstration of CYP1A and the ABC protein(s) gene expression and activity in European seabass (Dicentrarchus labrax) primary hepatocytes. Chemosphere 100: 152-159.
42. Popovic, M., Zaja, R., Fent, K., Smital, T. (2014) Interaction of environmental contaminants with zebrafish uptake transporter Oatp1d1 (Slco1d1). Toxicol. Appl. Pharmacol. 280: 149-158.
43. Glisic, B., Mihaljevic, I., Popovic, M., Zaja, R., Loncar, J., Fent, K., Kovacevic, R., Smital, T. (2015) Characterization of glutathione-S-transferases in zebrafish (Danio rerio). Aquat. Toxicol. 158: 50-62.
44. Lončar, J., Popović, M., Krznar, P., Zaja, R., Smital, T. (2016) The first characterization of multidrug and toxin extrusion (MATE/SLC47) proteins in zebrafish (Danio rerio). Sci. Rep. 6: 28937, DOI: 10.1038/srep28937.
45. Mihaljević, I., Popović, M., Zaja, R., Smital, T. (2016) Phylogenetic, syntenic, and tissue expression analysis of slc22 genes in zebrafish (Danio rerio). BMC Genomics, 17: 626, DOI 10.1186/s12864-016-2981-y.
46. Zaja, R., Popović, M., Lončar, J., Smital, T. (2016) Functional characterization of rainbow trout (Oncorhynchus mykiss) Abcg2a (Bcrp) transporter. Comp. Biochem. Physiol. C, 190: 15-23.
47. Mihaljević, I., Popović, M., Žaja, R., Maraković, N., Šinko, G., Smital, T. (2017) Interaction between the zebrafish (Danio rerio) organic cation transporter 1 (Oct1) and endo- and xenobiotics. Aquat. Toxicol. 187: 18-28.
48. Marić, P., Ahel, M., Senta, I., Terzić, S., Mikac, I, Žuljević, A., Smital, T. (2017) Effect-directed analysis reveals inhibition of zebrafish uptake transporter Oatp1d1 by caulerpenyne, a major secondary metabolite from the invasive marine alga Caulerpa taxifolia. Chemosphere 174: 643-654.
49. Dragojević, J., Mihaljević, I., Popović, M., Zaja, R., Smital, T. (2017) In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e. Toxicol In Vitro 46: 246-256.
50. Terzić, S., Udikovic-Kolić, N., Jurina, T., Krizman-Matašica, I., Senta, I., Mihaljević, I., Lončar, J., Smital, T., Ahel, M. (2018) Biotransformation of macrolide antibiotics using enriched activated sludge culture: Kinetics, transformation routes and ecotoxicological evaluation. J. Haz. Mat. 349: 143-152.
51. Lončar, J., Smital, T. (2018) Interaction of environmental contaminants with zebrafish (Danio rerio) multidrug and toxin extrusion protein 7 (Mate7/Slc47a7). Aquat. Toxicol. 205: 193-203.
52. Dean Karaica, Davorka Breljak, Jovica Lončar, Mila Lovrić, Vedran Micek, Ivana Vrhovac Madunić, Hrvoje Brzica, Carol M. Herak-Kramberger, Jana Ivković Dupor, Marija Ljubojević, Tvrtko Smital, Željka Vogrinc, Gerhard Burckhardt, Birgitta C. Burckhardt, Ivan Sabolić (2018) Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys. Arh. Hig. Rada Toksikol. 69: 286-303.
53. Kostanjevecki, P., Petric, I., Loncar, J., Smital, T., Ahel, M., Terzic, S. (2019) Biodegradation study of methadone by adapted activated sludge: elimination kinetics, transformation products and ecotoxicological evaluation. Chemosphere 214: 719-728.
54. Bašica, B., Mihaljević, I., Maraković, N., Kovačević, R., Smital, T. (2019) Molecular characterization of zebrafish Gstr1, the only member of teleost-specific glutathione S- transferase class. Aquat. Toxicol. 208: 196-207.
55. Kostanjevecki, P., Petric, I., Loncar, J., Smital, T., Ahel, M., Terzic, S. (2019) Aerobic biodegradation of tramadol by pre-adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment. Sci. Total. Environ. 687: 858-866.
56. Dragojević, J., Mihaljević, I., Popović, M., Smital, T. (2019) Zebrafish (Danio rerio) Oat1 and Oat3 transporters and their interaction with physiological compounds. Comp. Biochem. Physiol. B 236: 110309

Books and book chapters

Book chapters

• Smital, T. & Ahel, M. (2015) Ecotoxicological characterization of the Sava River – biomarker responses and biological assays. U: Milačić, R., Ščančar, J. & Paunović, M. (ur.) The Sava River. Berlin : Heidelberg, Springer, str. 177-200.

• Smital, T. (2008) Acute and Chronic Effects of Emerging Contaminants. U: Barcelo, D. & Petrovic, M. (ur.) Emerging Contaminants from Industrial and Municipal Waste - Occurrence, Analysis and Effects. Berlin, Springer, str. 154-193 doi:10.1007/978-3-540-74795-6_3.

  doi

• Epel, D. & Smital, T. (2001) Multidrug-multixenobiotic transporters and their significance with respect to environmental levels of pharmaceuticals and personal care products. U: Daughton, C. & Jones-Lepp, T. (ur.) Pharmaceuticals and personal care products in the environment - scientific and regulatory issues. Washington (MD), American Chemical Society (ACS) ; Oxford University Press, str. 244-263.

Journal articles

Scientific and review papers

• Karaica, D., Mihaljević, I., Vujica, L., Bošnjak, A., Dragojević, J., Otten, C., Babić, N., Lončar, J. & Smital, T. (2022) Stage‐dependent localization of F‐actin and Na + /K + ‐ ATPase in zebrafish embryos detected using optimized cryosectioning immunostaining protocol. Microscopy Research and Technique, 2022, 1-17 doi:10.1002/jemt.24270.

  doi

• Frleta, R., Popović, M., Smital, T. & Šimat, V. (2022) Comparison of Growth and Chemical Profile of Diatom Skeletonema grevillei in Bioreactor and Incubation-Shaking Cabinet in Two Growth Phases. Marine drugs, 20 (11), 697, 16 doi:10.3390/md20110697.

  doiwww.mdpi.com

• Marić, P., Ahel, M., Maraković, N., Lončar, J., Mihaljević, I. & Smital, T. (2021) Selective interaction of microcystin congeners with zebrafish (Danio rerio) Oatp1d1 transporter. Chemosphere, 283, 131155, 12 doi:10.1016/j.chemosphere.2021.131155.

  doi

• Dragojević, J., Marakovic, N., Popović, M. & Smital, T. (2021) Zebrafish (Danio rerio) Oatp2b1 as a functional ortholog of the human OATP2B1 transporter. Fish Physiology and Biochemistry, 47 (6), 1837-1849 doi:10.1007/s10695-021-01015-7.

  doiwww.scopus.com

• Dragojević, J., Marić, P., Lončar, J., Popović, M., Mihaljević, I. & Smital, T. (2020) Environmental contaminants modulate transport activity of zebrafish organic anion transporters Oat1 and Oat3. Comparative biochemistry and physiology. C. Toxicology & pharmacology, 231, 108742, 8 doi:10.1016/j.cbpc.2020.108742.

  doi

• Mihaljević, I., Bašica, B., Maraković, N., Kovačević, R. & Smital, T. (2020) Interaction of organotin compounds with three major glutathione S-transferases in zebrafish. Toxicology in vitro, 62, 104713, 9 doi:10.1016/j.tiv.2019.104713.

  doi

• Marić, P., Ahel, M., Babić, O., Simeunović, J. & Smital, T. (2020) Ecotoxicological profiling of selected cyanobacterial strains using multi-endpointeffect-directed analysis. Ecotoxicology, 2020, s10646-020-02201-8, 16 doi:10.1007/s10646-020-02201-8.

  doilink.springer.com

• Dragojević, J., Mihaljević, I., Popović, M. & Smital, T. (2019) Zebrafish (Danio rerio) Oat1 and Oat3 transporters and their interaction with physiological compounds. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 236, 110309, 11 doi:10.1016/j.cbpb.2019.110309.

  doi

• Kostanjevečki, P., Petrić, I., Lončar, J., Smital, T., Ahel, M. & Terzić, S. (2019) Aerobic biodegradation of tramadol by pre- adapted activated sludge culture: Cometabolic transformations and bacterial community changes during enrichment. Science of the total environment, 687, 858-866 doi:10.1016/j.scitotenv.2019.06.118.

  doi

• Bašica, B., Mihaljević, I., Maraković, N., Kovačević, R. & Smital, T. (2019) Molecular characterization of zebrafish Gstr1, the only member of teleost-specific glutathione S- transferase class. Aquatic toxicology, 208, 196-207 doi:10.1016/j.aquatox.2019.01.005.

  doi

• Kostanjevečki, P., Sviličić Petrić, I., Lončar, J., Smital, T., Ahel, M. & Terzić, S. (2019) Biodegradation study of methadone by adapted activated sludge: Elimination kinetics, transformation products and ecotoxicological evaluation. Chemosphere, 214, 719-728 doi:10.1016/j.chemosphere.2018.09.153.

  doi

• Karaica, D., Breljak, D., Lončar, J., Lovrić, M., Micek, V., Vrhovac Madunić, I., Brzica, H., Herak-Kramberger, C., Ivković Dupor, J., Ljubojević, M., Smital, T., Vogrinc, Ž., Burckhardt, G., Burckhardt, B. & Sabolić, I. (2018) Sex-independent expression of chloride/formate exchanger Cfex (Slc26a6) in rat pancreas, small intestine, and liver, and male-dominant expression in kidneys. Arhiv za higijenu rada i toksikologiju, 69 (4), 286-303 doi:10.2478/aiht-2018-69-3157.

  doihrcak.srce.hr

• Terzić, S., Udiković-Kolić, N., Jurina, T., Krizman-Matasić, I., Senta, I., Mihaljević, I., Lončar, J., Smital, T. & Ahel, M. (2018) Biotransformation of macrolide antibiotics using enriched activated sludge culture: Kinetics, transformation routes and ecotoxicological evaluation. Journal of hazardous materials, 349, 143-152 doi:10.1016/j.jhazmat.2018.01.055.

  doi

• Lončar, J. & Smital, T. (2018) Interaction of environmental contaminants with zebrafish multidrug and toxin extrusion protein 7 (Mate7/Slc47a7). Aquatic toxicology, 205, 193-203 doi:10.1016/j.aquatox.2018.10.016.

  doi

• Marić, P., Ahel, M., Senta, I., Terzić, S., Mikac, I., Žuljević, A. & Smital, T. (2017) Effect-directed analysis reveals inhibition of zebrafish uptake transporter Oatp1d1 by caulerpenyne, a major secondary metabolite from the invasive marine alga Caulerpa taxifolia. Chemosphere, 174, 643-654 doi:10.1016/j.chemosphere.2017.02.007.

  doi

• Mihaljević, I., Popović, M., Žaja, R., Maraković, N., Šinko, G. & Smital, T. (2017) Interaction between zebrafish Oct1 and endo- and xenobiotics. Aquatic toxicology, 187, 18-28 doi:10.1016/j.aquatox.2017.03.012.

  doi

• Dragojević, J., Mihaljević, I., Popović, M., Žaja, R. & Smital, T. (2017) In vitro characterization of zebrafish (Danio rerio) organic anion transporters Oat2a-e. Toxicology in vitro, 46, 246-256 doi:10.1016/j.tiv.2017.09.026.

  doi

• Žaja, R., Popović, M., Lončar, J. & Smital, T. (2016) Functional characterization of rainbow trout (Oncorhynchus mykiss) Abcg2a (Bcrp) transporter. Comparative biochemistry and physiology. C. Toxicology & pharmacology, 190, 15-23 doi:10.1016/j.cbpc.2016.07.005.

  doi

• Mihaljević, I., Popović, M., Žaja, R. & Smital, T. (2016) Phylogenetic, syntenic, and tissue expression analysis of slc22 genes in zebrafish (Danio rerio). Bmc genomics, 17, 626-1 doi:10.1186/s12864-016-2981-y.

  doi

• Lončar, J., Popović, M., Krznar, P., Žaja, R. & Smital, T. (2016) The first characterization of multidrug and toxin extrusion (MATE/SLC47) proteins in zebrafish (Danio rerio). Scientific Reports, 6, 28937, 15 doi:10.1038/srep28937.

  doi

• Glišić, B., Mihaljević, I., Popović, M., Žaja, R., Lončar, J., Fent, K., Kovačević, R. & Smital, T. (2015) Characterization of Glutathione-S-transferases in zebrafish (Danio rerio). Aquatic toxicology, 158, 50-62 doi:10.1016/j.aquatox.2014.10.013.

  doi

• Ferreira, M., Santosa, P., Rey-Salgueiroa, L., Žaja, R., Reis-Henriques, M. & Smital, T. (2014) The first demonstration of CYP1A and the ABC protein(s) gene expression and activity in European Seabass (Dicentrarchus labrax) primary hepatocytes. Chemosphere, 100, 152-159 doi:10.1016/j.chemosphere.2013.11.051.

  doi

• Popović, M., Žaja, R., Fent, K. & Smital, T. (2014) Interaction of environmental contaminants with zebrafish uptake transporter Oatp1d1 (Slco1d1). Toxicology and applied pharmacology, 280, 149-158 doi:10.1016/j.taap.2014.07.015.

  doi

• Traven, L., Mićović, V., Vukić Lušić, D. & Smital, T. (2013) The responses of the hepatosomatic index (HSI), 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione-S-transferase (GST) activity in sea bass (Dicentrarchus labrax, Linnaeus 1758) caged at a polluted site: implications for their use in environmental risk assessment. Environmental monitoring and assessment, 185 (11), 9009-9018 doi:10.1007/s10661-013-3230-3.

  doi

• Popović, M., Žaja, R., Fent, K. & Smital, T. (2013) Molecular characterization of zebrafish Oatp1d1 (Slco1d1), a novel Organic anion transporting polypeptide. The Journal of biological chemistry, 288 (47), 33894-33911 doi:10.1074/jbc.M113.518506.

  doi

• Bošnjak, I., Zaja, R., Sauerborn Klobučar, R., Šver, L., Franekić, J. & Smital, T. (2013) Identification of ABC Transporter Genes in Gonad Tissue of two Mediterranean Sea Urchin Species: Black, Arbacia lixula L., and Rocky, Paracentrotus lividus L.. Bulletin of environmental contamination and toxicology, 91 (4), 415-419 doi:10.1007/s00128-013-1021-8.

  doi

• Zaja, R., Terzić, S., Senta, I., Lončar, J., Popović, M., Ahel, M. & Smital, T. (2013) Identification of P-Glycoprotein Inhibitors in Contaminated Freshwater Sediments. Environmental science & technology, 47 (9), 4813-4821 doi:10.1021/es400334t.

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• Smital, T., Terzić, S., Lončar, J., Senta, I., Žaja, R., Popović, M., Mikac, I., Tollefsen, K., Thomas, K. & Ahel, M. (2013) Prioritisation of organic contaminants in a river basin using chemical analyses and bioassays. Environmental science and pollution research international, 20 (3), 1384-1395 doi:10.1007/s11356-012-1059-x.

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• Della Torre, C., Žaja, R., Lončar, J., Smital, T., Focardi, S. & Corsi, I. (2012) Interaction of ABC transport proteins with toxic metals at the level of gene and transport activity in the PLHC-1 fish cell line. Chemico-biological interactions, 198 (1/3), 9-17 doi:10.1016/j.cbi.2012.04.008.

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• Reifferscheid, G., Maes, H., Allner, B., Badurova, J., Belkin, S., Blum, K., Brauer, F., Bressling, J., Domeneghetti, S., Elad, T., Flückiger-Isler, S., Grummt, H., Guertler, R., Hecht, A., Heringa, M., Hollert, H., Huber, S., Kramer, M., Magdeburg, A., Ratte, T., Sauerborn-Klobucar, R., Sokolowski, A., Soldan, P., Smital, T., Stalter, D., Venier, P., Ziemann, C., Zipperle, J. & Buchinger, S. (2012) International round-robin study on the Ames fluctuation test. Environmental and molecular mutagenesis, 53 (3), 185-197 doi:10.1002/em.21677.

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• Ahel, M., Mikac, N. & Smital, T. (2011) Water resources and wastewater management in the Sava River Basin. Danube News, 13 (24), 13-15.

• Fischer, S., Lončar, J., Žaja, R., Schnell, S., Schirmer, K., Smital, T. & Luckenbach, T. (2011) Constitutive mRNAexpression and protein activity levels of nine ABC efflux transporters in seven permanent cell lines derived from different tissues of rainbow trout (Oncorhynchus mykiss). Aquatic toxicology, 101 (2), 438-446 doi:10.1016/j.aquatox.2010.11.010.

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• Žaja, R., Lončar, J., Popović, M. & Smital, T. (2011) First characterisation of fish P-glycoprotein (abcb1) substrate specificity using determinations of its ATPase activity and calcein-AM assay with PLHC-1/dox cell line. Aquatic toxicology, 103 (1/2), 53-62 doi:10.1016/j.aquatox.2011.02.005.

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• Smital, T., Terzić, S., Žaja, R., Senta, I., Pivčević, B., Popović, M., Mikac, I., Tollefsen, K., Thomas, K. & Ahel, M. (2011) Assessment of toxicological profiles of the municipal wastewater effluents using chemical analyses and bioassays. Ecotoxicology and environmental safety, 74 (4), 844-851 doi:10.1016/j.ecoenv.2010.11.010.

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• Bošnjak, I., Šegvić, T., Smital, T., Franekić Čolić, J. & Mladineo, I. (2011) Sea Urchin Embryotoxicity Test for Determination of Environmental Contaminants–Potential Role of the MRP Proteins. Water, air and soil pollution, 217 (1-4), 627-636 doi:10.1007/s11270-010-0615-6.

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• Sauerborn Klobučar, R., Žaja, R., Franjević, D., Brozović, A. & Smital, T. (2010) Presence of Ecotoxicologically Relevant Pgp- and MRP- Transcripts and Proteins in Cyprinid Fish. Arhiv za higijenu rada i toksikologiju, 61 (2), 175-181. (https://www.bib.irb.hr/446731).

  hrcak.srce.hr

• Popović, M., Žaja, R. & Smital, T. (2010) Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): phylogenetic analysis and tissue distribution. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 155 (3), 327-335 doi:10.1016/j.cbpa.2009.11.011.

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• Lončar, J., Popović, M., Žaja, R. & Smital, T. (2010) Gene expression analysis of the ABC efflux transporters in rainbow trout (Oncorhynchus mykiss). Comparative biochemistry and physiology. C. Toxicology & pharmacology, 151 (2), 209-215 doi:10.1016/j.cbpc.2009.10.009.

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• Popović, M., Žaja, R., Lončar, J. & Smital, T. (2010) A novel ABC transporter: the first insight into zebrafish (Danio rerio) Abch1. Marine environmental research, 69 (S1), S11-S13 doi:10.1016/j.marenvres.2009.10.016.

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• Bošnjak, I., Heim, W., Smital, T., Epel, D., Coale, K., Franekić-Čolić, J. & Hamdoun, A. (2009) Multidrug resistance associated protein transport activity mediates differences in accumulation and toxicity of inorganic and organic mercury in sea urchin embryos. Environmental science & technology, 43, 8374-8380.

• Traven, L., Žaja, R., Lončar, J., Smital, T. & Mićović, V. (2008) CYP1A induction potential and the concentration of priority pollutants in marine sediment samples – In vitro evaluation using the PLHC-1 fish hepatoma cell line. Toxicology in vitro, 22 (6), 1648-1656 doi:10.1016/j.tiv.2008.06.012.

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• Epel, D., Stevenson, C., MacManus-Spencer, L., Luckenbach, T., Hamdoun, A. & Smital, T. (2008) Efflux transporters: newly appreciated roles in protection against pollutants. Environmental science & technology, 42 (11), 3914-3920 doi:10.1021/es087187v.

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• Žaja, R., Munić, V., Sauerborn Klobučar, R., Ambriović-Ristov, A. & Smital, T. (2008) Cloning and molecular characterization of apical efflux transporters (ABCB1, ABCB11 and ABCC2) in rainbow trout (Oncorhynchus mykiss) hepatocytes. Aquatic Toxicology, 90 (4), 322-332.

• Žaja, R., Caminada, D., Lončar, J., Fent, K. & Smital, T. (2008) Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line. Toxicology and Applied Pharmacology, 227 (2), 207-218 doi:10.1016/j.taap.2007.11.001.

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• Žaja, R., Munić, V. & Smital, T. (2008) Cloning and mRNA expression analysis of an ABCG2 (BCRP) efflux transporter in rainbow trout (Oncorhynchus mykiss) liver and primary hepatocytes. Marine environmental research, 66 (1), 77-79 doi:10.1016/j.marenvres.2008.02.028.

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• Källqvist, T., Milačič, R., Smital, T., Thomas, K., Vranes, S. & Tollefsen, K. (2007) Chronic toxicity of the Sava River (SE Europe) sediments and river water to the algae Pseudokirchneriella subcapitata. Water Research, 42 (8-9), 2146-2156 doi:10.1016/j.watres.2007.11.026.

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• Žaja, R., Sauerborn Klobučar, R. & Smital, T. (2007) Detection and functional characterization of Pgp1 (ABCB1) and MRP3 (ABCC3) efflux transporters in the PLHC-1 fish hepatoma cell line. Aquatic toxicology, 81, 365-376.

• Žaja, R., Klobučar, G., Sauerborn Klobučar, R., Hackenberger, B. & Smital, T. (2006) Haemolymph as compartment for efficient and non-destructive determination of Pgp mediated MXR activity in bivalves. Comparative biochemistry and physiology. C. Toxicology & pharmacology, 143 (1), 103-112.

• Smital, T., Luckenbach, T., Sauerborn, R., Hamdoun, M., Vega, L. & Epel, D. (2004) Emerging contaminants - pesticides, PPCPs, microbial degradation products and natural substances as inhibitors of multixenobiotic defense in aquatic organisms. Mutation Research, 552, 101-117.

• Pivčević, B., Sauerborn Klobučar, R., Krča, S., Žaja, R., Jelaska, D., Smital, T. & Britvić Smiljana (2004) Methods of molecular biology in ecotoxicological research. Periodicum Biologorum, 106 (2), 121-125.

• Sauerborn, R., Stupin Polancec, D., Zaja, R. & Smital, T. (2004) Identification of the multidrug resistance-associated protein (mrp) related gene in red mullet (Mullus barbatus). Marine environmental research, 58 (2-5), 199-204 doi:10.1016/j.marenvres.2004.03.120.

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• Smital, T., Sauerborn, R. & Hackenberger, B. (2003) Inducibility of the multixenobiotic resistance mechanism (MXR) transport activity in the marine mussel Mytilus galloprovincialis and the freshwater mussel Dreissena polymorpha. Aquatic Toxicology, 65, 443-465.

• Smital, T. & Sauerborn, R. (2002) Measurement of the activity of multixenobiotic resistance mechanism in the common carp Cyprinus carpio. Marine environmental research, 54 (3/5), 449-453 doi:10.1016/S0141-1136(02)00155-1.

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• Ramljak, S., Hackenberger, B., Smital, T. & Britvić, S. (2000) Evaluation of the genotoxic and cytochrome P450 monooxigenase-inhibitory potential of Dicuran on procaryotic and eucaryotic test systems. Journal of Environmental Science and Health - Part B: Pesticides, Food Contaminants, and Agricultural Wastes, 35 (6), 751-770.

• Kurelec, B., Smital, T., Pivčević, B., Eufemia, N. & Epel, D. (2000) Multixenobiotic resistance, P-glycoprotein, and chemosensitizers. Ecotoxicology, 9 (5), 307-327.

• Smital, T., Sauerborn, R., Pivčević, B., Krča, S. & Kurelec, B. (2000) Interspecies differences in P-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates. Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology, 126, 175-186.

• Kurelec, B., Britvić, S., Pivčević, B. & Smital, T. (1998) Fragility of multixenobiotic resistance in aquatic organisms enhances the complexity of risk assessment. Marine environmental research, 46 (1-5), 415-419 doi:10.1016/S0141-1136(97)00065-2.

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• Smital, T. & Kurelec, B. (1998) The activity of multixenobiotic resistance mechanism determined by rhodamine B-efflux method as a biomarker of exposure. Marine environmental research, 46 (1-5), 443-447 doi:10.1016/S0141-1136(97)00087-1.

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• Osmak, M., Brozović, A., Ambriović-Ristov, A., Hadžija, M., Pivčević, B. & Smital, T. (1998) Inhibition of apoptosis is the cause of resistance to doxorubicin in human breast adenocarcinoma cells. Neoplasma, 45 (4), 223-230.

• Kurelec, B., Smital, T., Britvić, S., Pivčević, B., Krča, S., Jelaska, D., Balen, S., Sauerborn, R. & Mustajbegović, S. (1997) Multixenobiotic resistance mechanism in aquatic organisms. Periodicum biologorum, 99 (3), 319-328.

• Smital, T. & Kurelec, B. (1997) The concentrations of inhibitors of multixenobiotic resistance mechanism in natural waters: The direct in vivo demonstration of their effect. Environmental toxicology and chemistry, 16 (10), 2164-2170.

• Smital, T., Pivčević, B. & Kurelec, B. (1996) Reversal of multidrug resistance by extract from a marine algae Caulerpa taxifolia. Periodicum biologorum, 98 (2), 197-203.

Conference proceedings papers

Scientific conference proceedings papers

• Dražić, M., Bubalo, D., Smital, T., Krča, Sanja, Britvić, Smilja & Kezić, N. (2005) Expression of Multixenobiotic resistence of honeybee exposed to active compounds of medication drugs. U: Kovačević, V. & Jovanovac, S. (ur.)Proceedings, XL Croatian Symposium on Agriculture.

• Sauerborn, R., Stupin Polancec, D., Zaja, R. & Smital, T. (2003) Identification of the multidrug resistance-associated protein (MRP) related genes in several marine and freshwater fish species. U: 12th International Symposium on Pollutant Responses in Marine Organisms (PRIMO12) : conference proceedings.

Patents

• Kurelec, B., Smital, T. & Pivčević, B. (1996) Exstract from marine tropic alga introduced into Mediterranean, Caulerpa taxifolia, reverts multidrug resistance (MDR) in tumor cell culture.

Theses

Doctoral dissertation

• Smital, T. (1999) 'Activity of the multixenobiotic resistance mechanism in aquatic organisms as an indicator of the environmental quality', doktorska disertacija, Prirodoslovno-matematički fakultet, Zagreb.

Master thesis

• Smital, T. (1998) 'In vivo determination of the concentration of inhibitors of the multixenobiotic resistance mechanism (MXR) in river waters', magistarski rad, Prirodoslovno-matematički fakultet, Zagreb.

Member of Croatian Biological Society, Croatian Toxicological Society and International Society of Ecotoxicology and Environmental Safety (SECOTOX).