dr. sc. Dušica Vujaklija
Institut Ruđer Bošković
Bijenička 54
HR-10000 Zagreb
Obrazovanje
1992. Dr.sc. titula u području molekularne mikrobiologije stečena na Sveučilištu u Tokiju, Japan
1982. Dipl. inž. titula u području biokemijskog inženjerstva stečena na PBF-u, Sveučilišta u Zagrebu
Projekti
- 2019- HrZZ projekt "Projekt razvoja karijera mladih istraživača - izobrazba novih doktora znanosti"
- 2018-2022 HrZZ projekt ”Identifikacija interaktoma paralognih proteina SSB višestaničnom prokariotu, Streptomyces coelicolor” (IP-2018-01-1754), (D. Vujaklija voditeljica projekta), www.irb.hr/idrobust
- 2017-2022 “Bioprospecting Jadranskog mora” (KK.01.1.1.01, Fond za regionalni razvoj EU), D. Vujaklija voditeljica Aktivnosti 1: Istraživanje biološke raznolikosti i genetičkih resursa morskih staništa. http://bioproadriatic.hr/
- 2015-2020 Znanstveni centar izvrsnosti za bioprospecting mora – BioProCro. D Vujaklija, voditeljica Aktivnosti 1, ”Istraživanje biološke raznolikosti“ http://bioprocro.zci.hr/
Bilateralni projekti:
- 2020-2022 DAAD-RH bilaterarni projekt „Novi pristup za evaluaciju vezanja ssDNA i paralognih proteina SSB iz bakterije Streptomyces coelicolor“
- 2019-2020 Bilateralni projekt /Hrvatsko–Francuski Program "Cogito" - Partnerstvo Hubert Curien: „Genetička raznolikost mikrobne populacije ibakteriofaga u odabranim nišama Jarandkog mora“.
Nagrade i priznanja
2020 Plaketa Hrvatskog mikrobiološkog društva
2013 Godišnja nagrada Instituta Ruđer Bošković za najbolji rad znanstvenika s doktoratom
2007 Nagradu „Josip Juraj Strossmayer” dobio je IRB za knjigu “Metode u molekularnoj biologiji” u području znanosti u RH (D. Vujaklija – jedna od urednica)
Nastava
- 2009 - Molekularni principi genetičkog inženjerstva (D. Vujaklija, sunositeljica kolegija), Veterinarski fakultet, Sveučilište u Zagrebu
Istaknute publikacije
- Bermanec, V., Paradžik, T., Kazazić, S.P.; Venter, C., Hrenović., Vujaklija, D*., Duran, R., Boev, I., B. Boev, Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia, Journal of Hazardous Materials, 2021, 402, 123902, 10 doi:10.1016/j.jhazmat.2020.123437 (D. Vujaklija, dopisni autor)
- Biđin, S., I. Vujaklija, T. Paradžik, A. Bielen, D. Vujaklija* Leitmotif: protein motif scanning 2.0., Bioinformatics, 2020; 36(11): 3566-2020. (D. Vujaklija, dopisni autor)
- Vujaklija I*, A Bielen*,T Paradžik, S Biđin, P Goldstein and D Vujaklija*, An effective approach for annotation of protein families with low sequence similarity and conserved motifs: identifying GDSL hydrolases across the plant kingdom, BMC Bioinformatics, 2016, 18;17:91 (D. Vujaklija, dopisni autor)
- Paradzik T, N. Ivic, Z Filic, Babu A. Manjasetty, P Herron, M Luic and D Vujaklija*, Structure-function relationships of two paralogous single-stranded DNA binding proteins from Streptomyces coelicolor: implication of SSB-B in chromosome segregation during sporulation, Nucleic Acids Res., 2013, 1;41(6):3659-72 (D. Vujaklija, dopisni autor)
- Stefanic Z, D. Vujaklija and M. Luic, Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor, Acta Cryst. 2009, D65, 974-979.
- Mijaković, I., Petranović, D., Maček, B., Čepo, T., Mann, M., Davies, J., Jensen, P. R., Vujaklija, D*. Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. Nucleic Acids Research 2006, 34(5) 1588-1596 (D. Vujaklija, dopisni autor)
- Petkovič, H., Cullum, J. Hranueli, D., Hunter, I.S., Perić-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D. Long, P.F. Genetics of Streptomyces rimosus, the oxytetracycline producer. Microbiology and Molecular Biology Reviews, 2006,70(3), 704-728
Publikacije
Knjige i poglavlja u knjigama
Poglavlja u knjigama
-
Vujaklija, D. & Maček, B. (2012) Detecting Posttranslational Modifications of Bacterial SSB Proteins. U: Keck, J. (ur.) Single-Stranded DNA Binding Proteins. New York, Humana Press - Springer, str. 205-218 doi:10.1007/978-1-62703-032-8_16.
doi Vujaklija, D. (2007) Prijenos plazmidne DNA između raznorodnih bakterija metodom elektrodukcije. U: Ambriović Ristov, A., Bruvo Mađarić, B., Ćetković, H., Hranilović, D., Herak Bosnar, M., Katušić Hećimović, S., Meštrović Radan, N., Mihaljević, S., Slade, N. & Vujaklija, D. (ur.) Metode u molekularnoj biologiji. Zagreb, Institut Ruđer Bošković, str. 931-933.
Vujaklija, D. (2007) Izdvajanje genomske DNA iz streptomiceta. U: Ambriović Ristov, A., Bruvo Mađarić, B., Ćetković, H., Hranilović, D., Herak Bosnar, M., Katušić Hećimović, S., Meštrović Radan, N., Mihaljević, S., Slade, N. & Vujaklija, D. (ur.) Metode u molekularnoj biologiji. Zagreb, Institut Ruđer Bošković, str. 144-146.
Radovi u časopisima
Znanstveni i pregledni radovi
-
Hađina, S., Bruvo Mađarić, B., Kazazić, S., Paradžik, T., Reljić, S., Pinter, L., Huber, Đ. & Vujaklija, D. (2023) Malassezia pachydermatis from brown bear: A comprehensive analysis reveals novel genotypes and distribution of all detected variants in domestic and wild animals. Frontiers in microbiology, 14, 1151107, 12 doi:10.3389/fmicb.2023.1151107.
doi -
Šarić, E., Quinn, G., Nalpas, N., Paradžik, T., Kazazić, S., Filić, Ž., Šemanjski, M., Herron, P., Hunter, I., Maček, B. & Vujaklija, D. (2022) Phosphoproteome Dynamics of Streptomyces rimosus during Submerged Growth and Antibiotic Production. mSystems, 7 (5), 1-20 doi:10.1128/msystems.00199-22.
doijournals.asm.orgfulir.irb.hr -
Bermanec, V., Paradžik, T., Kazazić, S., Venter, C., Hrenović, J., Vujaklija, D., Duran, R., Boev, I. & Boev, B. (2021) Novel arsenic hyper-resistant bacteria from an extreme environment, Crven Dol mine, Allchar, North Macedonia. Journal of hazardous materials, 402, 123902, 10 doi:10.1016/j.jhazmat.2020.123437.
doi -
Biđin, S., Vujaklija, I., Paradžik, T., Bielen, A. & Vujaklija, D. (2020) Leitmotif: protein motif scanning 2.0. Bioinformatics, 36 (11), 3566-3567 doi:10.1093/bioinformatics/btaa133.
doi -
Terra, L., Dyson, P., Hitchings, M., Thomas, L., Abdelhameed, A., Banat, I., Gazze, S., Vujaklija, D., Facey, P., Francis, L. & Quinn, G. (2018) A Novel Alkaliphilic Streptomyces Inhibits ESKAPE Pathogens. Frontiers in microbiology, 9 (OCT), 2458, 13 doi:10.3389/fmicb.2018.02458.
doiwww.frontiersin.org -
Đermić, E., Zahradka, D., Vujaklija, D., Ivanković, S. & Đermić, D. (2017) 3'-terminated Overhangs Regulate DNA Double-Strand Break Processing in Escherichia coli. G3-Genes Genomes Genetics, 7 (9), 3091-3102 doi:10.1534/g3.117.043521.
doi -
Ivanković, S., Vujaklija, D. & Đermić, D. (2017) Nucleolytic degradation of 3′-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli. DNA repair, 57, 56-65 doi:10.1016/j.dnarep.2017.06.024.
doifulir.irb.hr -
Paradžik, T., Filić, Ž. & Vujaklija, D. (2016) Variations in amino acid composition in bacterial single stranded DNA–binding proteins correlate with GC content. Periodicum biologorum, 118 (4), 385-397 doi:10.18054/pb.v118i4.4847.
doihrcak.srce.hr -
Vujaklija, I., Bielen, A., Paradžik, T., Biđin, S., Goldstein, P. & Vujaklija, D. (2016) An effective approach for annotation of protein families with low sequence similarity and conserved motifs : identifying GDSL hydrolases across the plant kingdom. BMC bioinformatics, 17, 91-1 doi:10.1186/s12859-016-0919-7.
doi -
Abella, J., Bielen, A., Huang, L., Delmont, T., Vujaklija, D., Duran, R. & Cagnon, C. (2015) Integron diversity in marine environments. Environmental science and pollution research international, 22 (20), 15360-15369 doi:10.1007/s11356-015-5085-3.
doi -
Duran, R., Bielen, A., Paradžik, T., Gassie, C., Pustijanac, E., Cagnon, C., Hamer, B. & Vujaklija, D. (2015) Exploring Actinobacteria assemblages in coastal marine sediments under contrasted human influences in the West Istria Sea, Croatia. Environmental science and pollution research international, 22 (20), 15215-15229 doi:10.1007/s11356-015-4240-1.
doi -
Bielen, A., Teparić, R., Vujaklija, D. & Mrša, V. (2014) Microbial anchoring systems for cell-surface display of lipolytic enzymes. Food technology and biotechnology, 52 (1), 16-34.
hrcak.srce.hrfulir.irb.hr -
Paradžik, T., Ivić, N., Filić, Ž., Manjasetty, B., Herron, P., Luić, M. & Vujaklija, D. (2013) Structure–function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor : implication of SsbB in chromosome segregation during sporulation. Nucleic acids research, 41 (6), 3659-3672 doi:10.1093/nar/gkt050.
doi -
Leščić Ašler, I., Pigac, J., Vujaklija, D., Luić, M. & Štefanić, Z. (2011) Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4-dichloroisocoumarin. Acta crystallographica. Section F, Structural biology and crystallization communications, 67 (11), 1378-1381 doi:10.1107/S1744309111032222.
doi -
Goldstein, P., Žučko, J., Vujaklija, D., Kriško, A., Hranueli, D., Long, P., Etchebest, C., Basrak, B. & Cullum, J. (2009) Clustering of protein domains for functional and evolutionary studies. BMC bioinformatics, 10, 335, 11 doi:10.1186/1471-2105-10-335.
doibmcbioinformatics.biomedcentral.com -
Štefanić, Z., Vujaklija, D. & Luić, M. (2009) Structure of the single-stranded DNA-binding protein from Streptomyces coelicolor. Acta crystallographica. Section D, Biological crystallography, 65 (9), 974-979 doi:10.1107/S0907444909023634.
doi Bielen, A., Ćetković, H., Long, P., Schwab, H., Abramić, M. & Vujaklija, D. (2009) The SGNH-hydrolase of Streptomyces coelicolor has (aryl)esterase and a true lipase activity.. Biochimie, 91 (3), 390-400.
-
Castaldo, G., Žučko, J., Heidelberger, S., Vujaklija, D., Hranueli, D., Wattana-Amorn, P., Crump, P., Crosby, J. & Long, F. (2008) Proposed arrangement of proteins forming a bacterial type II polyketide synthase. Chemistry & biology, 15 (11), 1156-1165 doi:10.1016/j.chembiol.2008.09.010.
doi -
Salopek-Sondi, B., Vukelić, B., Špoljarić, J., Šimaga, Š., Vujaklija, D., Makarević, J., Jajčanin, N. & Abramić, M. (2008) Functional tyrosine residue in the active center of human dipeptidyl peptidase III. Biological chemistry, 389 (2), 163-167 doi:10.1515/BC.2008.021.
doi -
Štefanić, Z., Vujaklija, D., Andrišić, L., Mikleušević, G., Andrejašič, M., Turk, D. & Luić, M. (2007) Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein. Croatica chemica acta, 80 (1), 35-39. (https://www.bib.irb.hr/300532).
fulir.irb.hr -
Petković, H., Cullum, J., Hranueli, D., Hunter, S., Perić-Concha, N., Pigac, J., Thamchaipenet, A., Vujaklija, D. & Long, F. (2006) Genetics of Streptomyces rimosus, the Oxytetracycline Producer. Microbiology and Molecular Biology Reviews, 70 (3), 704-728 doi:10.1128/MMBR.00004-06.
doi Mijaković, I., Petranović, D., Maček, B., Čepo, T., Mann, M., Davies, J., Jensen, P. & Vujaklija, D. (2006) Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine. NUCLEIC ACIDS RESEARCH, 34 (5), 1588-1596.
-
Orhanović, S., Bučević-Popović, V., Pavela-Vrančič, M., Vujaklija, D. & Gamulin, V. (2006) Effect of a Thr81→ Ala Mutation at the Subunit Effect of a T81A mutation at the subunit interface on catalytic properties of alkaline phosphatase from Escherichia coli. International journal of biological macromolecules, 40 (1), 54-58 doi:10.1016/j.ijbiomac.2006.06.008.
doi Ishiyama, D., Vujaklija, D. & Davies, J. (2004) Novel Pathway of Salycilate Degradation by Streptomyces sp. Strain WA46. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70 (3), 1297-1306.
Vujaklija, D., Abramić, M., Leščić, I., Maršić, T. & Pigac, J. (2003) Streptomyces rimosus GDS(L) lipase: production, heterologous overexpression and structure-stability relationship. Food Technology and Biotechnology, 41 (1), 89-94.
Vujaklija, D., Schroeder, W., Abramić, M., Zou, P., Leščić, I., Franke, P. & Pigac, J. (2002) A novel streptomycete lipase: cloning, sequencing and high-level expression of the Streptomyces rimosus GDS(L)-lipase gene. Archives of Microbiology, 178 (2), 124-130.
-
Ahel, I., Vujaklija, D., Mikoč, A. & Gamulin, V. (2002) Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of a new type of promoter. FEMS microbiology letters, 209 (1), 129-133 doi:10.1111/j.1574-6968.2002.tb11121.x.
doi Bakal, C., Vujaklija, D. & Davies, J. (2001) Immunochemical Identification of a Stat3 Analogue in Streptomycetes. Food Technology and Biotechnology, 39 (4), 313-317.
Gregorović, G. & Vujaklija, D. (2001) High efficiency of direct plasmid transfer between two Streptomyces spp., and between Streptomyces spp. and E. coli. Food Technology and Biotechnology, 39 (1), 49-53.
-
Mikoč, A., Vujaklija, D. & Gamulin, V. (1997) The recA gene from Streptomyces rimosus R6: sequence and expression in Escherichia coli. Research in microbiology, 148 (5), 397-403 doi:10.1016/S0923-2508(97)83870-3.
doi -
Vujaklija, D., Žafran, J., Mikoč, A. & Gamulin, V. (1996) Direct detection of extracellular deoxyribonuclease in different strains of Streptomyces rimosus. Food technology and biotechnology, 34 (2-3), 71-76.
www.ftb.com.hr -
Vujaklija, D. & Davies, J. (1995) Direct transfer of plasmid DNA between Streptomyces spp. and Echerichia coli by electroduction. Journal of antibiotics, 48 (7), 635-637 doi:10.7164/antibiotics.48.635.
doi -
Waters, B., Vujaklija, D., Gold, M. & Davies, J. (1994) Protein tyrosine phosphorylation in Streptomycetes. FEMS microbiology letters, 120 (1/2), 187-190 doi:10.1111/j.1574-6968.1994.tb07028.x.
doi -
Vujaklija, D., Horinouchi, S. & Beppu, T. (1993) Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. Journal of bacteriology, 175 (9), 2652-2661 doi:10.1128/jb.175.9.2652-2661.1993.
doi -
Vujaklija, D., Ueda, K., Hong, S., Beppu, T. & Horinouchi, S. (1991) Identification of an A-factor-dependent promoter in the streptomycin biosynthetic gene cluster of Streptomyces griseus. MGG. Molecular & general genetics, 229 (1), 119-128 doi:10.1007/BF00264220.
doi -
Pigac, J., Vujaklija, D., Toman, Z., Gamulin, V. & Schrempf, H. (1988) Structural instability of a bifunctional plasmid pZG1 and single-stranded DNA formation in Streptomyces. Plasmid, 19 (3), 222-230 doi:10.1016/0147-619X(88)90040-6.
doi
Radovi u zbornicima skupova
Znanstveni radovi u zbornicima skupova
Durmiši, E., Vujaklija, D., Bielen, A., Duran, R. & Hamer, B. (2013) Physicochemical and ecotoxicological evaluation of the Rovinj costal area sediments, northern Adriatic, Croatia. U: de Lange, G., Gačić, M., Romaña, A., da Costa, M., Boero, F., Tura, C. & Sala, E. (ur.)Rapport Commission International pour l'exploration scientifique de la Mer Mediterranee.
Članstva u profesionalnim udrugama / društvima
- Hrvatsko genetičko društvo - HGD (2009 - član Upravnog odbora, 2015-2019 dopredsjednica)
- Hrvatsko društvo za biokemiju i molekularnu biologiju - HDBMB
- Hrvatsko mikrobiološko društvo - HMD (2012-2020. Predsjednica Bakteriološke sekcije, 2012.- član Povjerenstva za godišnje nagrade, 2019 - član Povjerenstva za skupove, 2015 - član Izdavačkog savjeta)
- Hrvatsko društvo za matematičku i teorijsku biologiju - HDMTB
- European Environmental Mutagen Society - EEMS