SMART-NANO will develop an innovative, cost-effective technology platform that provides a total solution “from sample-to-result” for the detection, identification, and measurement of nanoparticles in complex matrices in Consumer Products, in Food, in the Environment and in situ in Biota. A key innovation is the miniaturized, application-specific, cartridge-based system integrating separation, detection, and quantification. On top of this CORE innovation, plug-in modules for sample preparation, high sensitivity size measurement, and hypersensitive identification, provide the necessary sensitivity and flexibility to this technology platform. Highly innovative approaches also lie in the supercritical CO2 isolation of Engineered Nanoparticles (ENPs) from complex matrices and the ICP-MS based hypersensitive identification of ENPs.A practical approach based on the development of the technology platform together with the development and field testing of methods and protocols will result in ready-to-use, cost-effective cartridges for immediate, widespread use in applications for real life detection and measurement of Engineered Nanoparticles.The consortium is led by a non-profit research organization whose mission is to transfer technological innovation to the market (CSEM), has a strong participation of a supernational research center whose mission is to provide scientific support to EU policy makers (JRC), includes five SMEs (FeyeCon Carbon Dioxide Technologies, Postnova Analytics GmbH, Avid Nano Ltd, AHAVA Dead Sea Laboratories Ltd, ABICH S.r.l.) and a public research organization (Ruđer Bošković Institute) with a track record of excellence and innovation in the different analytical steps necessary to carry out the isolation, separation and measurement of nanoparticles in complex matrices. The SMART-NANO consortium has thus the required vision and experience to successfully execute this innovative project.