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High-performance liquid chromatography with mass spectrometer (HPLC-MS)

Kategorija
  • Farmaceutska industrija
  • Medicina
  • Zaštita okoliša
  • Prehrambena industrija
  • Kemijska i petrokemijska industrija
  • Usluge raznih namjena

The system consists of an Agilent 1200 Series HPLC system with a DAD detector and an Agilent 6420 triple quadrupole mass spectrometer.

Ionization with scattered electrons (Electrospray Ionisation - ESI)
Multimode source (ESI and APCI)

Recording modes:

MS scan
Selective Ion Monitoring (SIM)
MS/MS product ion
MS/MS precursor ion
Neutral gain/loss
Multiple Reaction Monitoring (MRM)
Possibilities of working on the MS/MS system:
Qualitative analysis

• recording of the spectrum of the sample
• determination of the molecular weight of the compound
• fragmentation analysis of a specific molecular ion (product ion)
• determination of the precursor of a specific fragment (precursor ion)

Quantitative analysis

• using standard analyte solutions and an internal standard, a calibration curve is created using the MRM technique, on the basis of which the concentration in the samples is determined

Terms of use

According to the agreement with the laboratory staff, by filling out the attached form.

Price list

For users from the IRB 8.76 EUR/hour
For users within the Croatian scientific system, but outside the IRB 21.77 EUR/hour + VAT
For users outside the Croatian scientific system 54.95 EUR/hour + VAT

Instrument location
IRB, II. wing, room 131

Contact:

Dr Ivanka Jerić, e-mail: ijeric@irb.hr
tel. +385 1 4560 998
fax. +385 1 4680 195

Dr Lidija Brkljačić, e-mail: lidija.brkljacic@irb.hr
tel. +385 1 4560 998
fax. +385 1 4680 195

MS info day-prezentacija