MARILIA - YOUR SAFE WATER DROP We are building novel pathogen detection test for the analysis of water samples
The state-of-the-art in water testing is based on cultivation-based techniques that require one or more days until results are obtained. In classical approaches, water is filtered using a membrane that retains bacteria cells. The membrane is subsequently incubated on selective media. Commercial reporter enzyme-based assays such as the Colilert and Enterolert are also used to identify single types of species. Due to the high economic pressure, other, more sophisticated molecular techniques such as PCR are rarely used for routine tests. As a result, the cost-effective, cultivation-based identification of pathogens using selective media is more frequently applied.
In MARILIA, we aim to develop a breakthrough pathogen detection assay for monitoring water safety that significantly increases the speed and assay performance. Especially the number of pathogenic species that can be detected with a single test will be increased, making it possible to use only a single assay for water safety testing.
The reduction of labour-intense cultivation-steps that, up to now, have to be conducted separately for every target species will also massively reduce personnel cost and the amount of required consumables. In addition to advances concerning cost, speed and the multiplexing capability, our most ambitious goal is the development of a quantitative method that enables the detection of even single cells. The prototype that will be developed during this project can transform the water testing market. However, we foresee a very high potential of the detection technology also for other products and markets.
The fast, sensitive, and cost-effective detection of pathogens is also highly important in many other sectors, such as the food, agricultural, and healthcare sector. Once the detection system is established using water samples, alternative sample sources will be targeted after the end of the project. The technology and consumables of the pathogen detection assay will remain the same because the pathogens are the same.
Due to cost-intensive regulation requirements in the healthcare sector, we have decided to develop products for less regulated markets such as the water and food sector first.
After establishing successful products in these sectors, a start-up will also explore options to enter the human diagnostic market due to its huge potential.
Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina (BioMultiChromoProbes)
HrZZ projekt br. 5475
"Višekromoforne probe za prepoznavanje pojedinih struktura DNA, RNA i proteina"
Multifunctional molecular sensing of DNA/RNA secondary structure by chemical sensors - DNA/RNA-MolSense
Molecular recognition of nucleic acids is of fundamental importance e.g. for gene transcription and regulation, protein synthesis or cell-division. Small molecules selectively targeting specific DNAs or RNAs are therefore highly interesting not only to understand such processes in more detail on a molecular level but also for potential medicinal applications, as sensors or probes for fluorescence based bioimaging as well as for analytical purposes in isolated biosamples.
HAMAG-BICRO Program provjere inovativnog koncepta: PoC6_11_47 "Nova dijagnostička metoda za demenciju Alzheimerovog tipa"
Ovim projektom provjerit će se validnost nove dijagnostičke metode za demencije Alzheimerovog tipa. Na projektu sudjeluju znanstvenici LBIS i Laboratorija za molekularnu neuropsihijatriju (LMNP), partneri su specijalisti i znanstvenici Zavoda za biologijsku psihijatriju i psihogerijatriju, Klinike za psihijatriju Vrapče
Europski socijalni fond ESF - HR.3.2.01-0254 "Jačanje kapaciteta u medicinskoj kemiji i istraživanju bioaktivnih sustava".
Projektni tim Instituta Ruđer Bošković sastavljen od pet mladih istraživača i poslijedoktoranada provesti će konkurentno znanstveno istraživanja u područja medicinske kemije relevantno za farmaceutsku industriju Republike Hrvatske i sektor zdravlja. Jačanje upravljačkih i istraživačkih kompetencija mladih istraživača i poslijedoktoranada provest će se edukacijom na odabranim stručnim radionicama. Diseminacija rezultata projekta provest će se u cilju ostvarenja suradnje sa znanstvenicima u Europskom istraživačkom prostoru i s poslovnim sektorom Republike Hrvatske.